Abstract
For an overview on the occurrence of Giardia assemblages in children in Eastern Slovakia, we examined 259 faecal samples of children from the segregated settlement in Medzev, 30 samples of children from the orphanage in Medzev and 40 samples of children with autism from the Special Elementary School in Košice. Thirty-eight samples (14.67 %) from the segregated settlement, 19 samples (63.33 %) from the orphanage and two samples (5.0 %) from the Special Elementary School were positive for Giardia by flotation. The initial microscopic diagnostics were completed by the genotyping of the triosephosphate isomerase-gene loci (tpi genes) which revealed the existence of two Giardia assemblages in Slovak population, namely Giardia duodenalis (assemblage A) and Giardia enterica (assemblage B). These results represent the first evidence of A and B assemblages in children in Slovakia. Epidemiological significance and the impact on the public health of Giardia infection are highlighted.
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Acknowledgments
This study was funded by the projects of the Scientific Grant Agency of the Ministry of Education of the SR and the Slovak Academy of Sciences, VEGA 1/0455/15 and No. 26220220152 implementation supported by the Research & Development Operational Programme funded by the ERDF.
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Štrkolcová, G., Goldová, M., Maďar, M. et al. Giardia duodenalis and Giardia enterica in children: first evidence of assemblages A and B in Eastern Slovakia. Parasitol Res 115, 1939–1944 (2016). https://doi.org/10.1007/s00436-016-4935-3
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DOI: https://doi.org/10.1007/s00436-016-4935-3