Abstract
Dirofilaria repens causes subcutaneous infection in dogs and cats, and is the main agent of human dirofilariosis in Europe. Detection of D. repens is described in a dog from Portugal, a finding that simultaneously represents the first presumable case of autochthonous infection with the parasite in any animal host species in the country. A mixed D. repens/Dirofilaria immitis infection (20:1 ratio) was found, with an overall density of 1267 microfilariae per millilitre of blood. Morphological features, including morphometry, and acid phosphatase histochemical staining confirmed identity of the two filarioids. Distribution of D. repens in Portugal should be further assessed at the population level both in vertebrate hosts and in vectors. Due to the zoonotic potential of the parasite, preventive measures such as the administration of microfilaricides and insect repellents should be put into practice to protect animals and public health.
References
Alho AM, Nunes T, Rinaldi L, Meireles J, Belo S, Deplazes P, de Carvalho LM (2014) Transmission risk of dirofilariosis in Portugal. Parasit Vectors 7(Suppl 1):O16
Antolová D, Miterpáková M, Paraličová Z (2015) Case of human Dirofilaria repens infection manifested by cutaneous larva migrans syndrome. Parasitol Res 114:2969–2973
Araújo AM (1996) Canine and human Dirofilaria immitis in Portugal. A review. Parassitologia 38:366 (abstract)
Baptista-Fernandes T, Rodrigues M, Domingues D, Monteiro L, Paixão P, Pereira P, Tavares R, Rodrigues P, Maurício I, Belo S, Toscano C (2015) Dirofilariasis by Dirofilaria repens: an imported case and a brief review. Parasitol Int 64:261–263
Bocková E, Iglódyová A, Kočišová A (2015). Potential mosquito (Diptera:Culicidae) vector of Dirofilaria repens and Dirofilaria immitis in urban areas of Eastern Slovakia. Parasitol Res (in press)
Cardoso L, Mendão C, Madeira de Carvalho L (2012) Prevalence of Dirofilaria immitis, Ehrlichia canis, Borrelia burgdorferi sensu lato, Anaplasma spp. and Leishmania infantum in apparently healthy and CVBD-suspect dogs in Portugal–a national serological study. Parasit Vectors 5:62
Ferreira CA, de Pinho MV, Novo MT, Calado MM, Gonçalves LA, Belo SM, de Almeida AP (2015) First molecular identification of mosquito vectors of Dirofilaria immitis in continental Portugal. Parasit Vectors 8:139
Giangaspero A, Marangi M, Latrofa MS, Martinelli D, Traversa D, Otranto D, Genchi C (2013) Evidences of increasing risk of dirofilarioses in Southern Italy. Parasitol Res 112:1357–1361
Giudice E, Di Pietro S, Gaglio G, Di Giacomo L, Bazzano M, Mazzullo G (2014) Adult of Dirofilaria repens in a dog with recurrent multiple subcutaneous nodular lesions. Parasitol Res 113:711–716
Harizanov RN, Jordanova DP, Bikov IS (2014) Some aspects of the epidemiology, clinical manifestations, and diagnosis of human dirofilariasis caused by Dirofilaria repens. Parasitol Res 113:1571–1579
Ionică AM, Matei IA, Mircean V, Dumitrache MO, D’Amico G, Győrke A, Pantchev N, Annoscia G, Albrechtová K, Otranto D, Modrý D, Mihalca AD (2015) Current surveys on the prevalence and distribution of Dirofilaria spp. and Acanthocheilonema reconditum infections in dogs in Romania. Parasitol Res 114:975–982
Little SE, Munzing C, Heise SR, Allen KE, Starkey LA, Johnson EM, Meinkoth J, Reichard MV (2014) Pre-treatment with heat facilitates detection of antigen of Dirofilaria immitis in canine samples. Vet Parasitol 203:250–252
Maia C, Ramos C, Coimbra M, Cardoso L, Campino L (2015) Prevalence of Dirofilaria immitis antigen and antibodies to Leishmania infantum in cats from southern Portugal. Parasitol Int 64:154–156
Menn B, Lorentz S, Naucke TJ (2010) Imported and travelling dogs as carriers of canine vector-borne pathogens in Germany. Parasit Vectors 3:34
Otranto D, Dantas-Torres F, Brianti E, Traversa D, Petrić D, Genchi C, Capelli G (2013) Vector-borne helminths of dogs and humans in Europe. Parasit Vectors 6:16
Pantchev N, Etzold M, Daugschies A, Dyachenko V (2011) Diagnosis of imported canine filarial infections in Germany 2008–2010. Parasitol Res 109(Suppl 1):S61–S76
Rombert PC, Nunes J, Azevedo V, Sinari V (1992) Um caso de dirofilariose ocular. In: Proceedings of the 1st Journeys of Infectious Diseases and Tropical Medicine. Institute of Hygiene and Tropical Medicine, Lisbon (abstract).
Rudolf I, Šebesta O, Mendel J, Betášová L, Bocková E, Jedličková P, Venclíková K, Blažejová H, Šikutová S, Hubálek Z (2014) Zoonotic Dirofilaria repens (Nematoda: Filarioidea) in Aedes vexans mosquitoes, Czech Republic. Parasitol Res 113:4663–4667
Sassnau R, Czajka C, Kronefeld M, Werner D, Genchi C, Tannich E, Kampen H (2014) Dirofilaria repens and Dirofilaria immitis DNA findings in mosquitoes in Germany: temperature data allow autochthonous extrinsic development. Parasitol Res 113:3057–3061
Acknowledgments
Carla Maia (SFRH/BPD/44082/2008) holds a scholarship from Fundação para a Ciência e a Tecnologia, Ministério da Educação e Ciência, Portugal. The work of C. Maia, L. Cardoso and D. Otranto was done under the frame of EurNegVec COST Action TD1303.
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Maia, C., Lorentz, S., Cardoso, L. et al. Detection of Dirofilaria repens microfilariae in a dog from Portugal. Parasitol Res 115, 441–443 (2016). https://doi.org/10.1007/s00436-015-4796-1
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DOI: https://doi.org/10.1007/s00436-015-4796-1