Abstract
Anthelmintic resistance against most of the commercial drugs is a great threat to humans as well as the veterinary live stocks. Hence, new treatment strategies to control helminth infections are essential at this hour. Carex baccans Nees has been traditionally used by Jaintia tribes in Northeast India to get rid of intestinal worm infections. Therefore, the present study was conducted to evaluate in vivo cestocidal activity of root tuber extract of C. baccans and its active component resveratrol against the zoonotic cestode Hymenolepis diminuta in the experimental model rat. The cestocidal activity was determined by monitoring the eggs per gram (EPG) counts in faeces of different treated groups. The result showed that the highest dose of the plant extract (50 mg/kg) and resveratrol (4.564 mg/kg body weight) has significant anthelmintic efficacy against H. diminuta. Crude extract of the plant as well as resveratrol reduced EPG count (56.012 and 46.049 %) and also resulted in decreased worm burden by 44.287 and 31.034 %, respectively. The efficacy of the crude extract and resveratrol can be compared to the reference drug praziquantel. The results exhibits considerable cestocidal potential of root tuber crude extract of C. baccans and resveratrol and justify its folklore use.
This is a preview of subscription content, log in via an institution to check access.
References
Abdel-Ghaffar F, Semmler M, Al-Rasheid KA, Strassen B, Fischer K, Aksu G, Klimpel S, Mehlhorn H (2011) The effects of different plant extracts on intestinal cestodes and on trematodes. Parasitol Res 108(4):979–984
Baur JA, Sinclair DA (2006) Therapeutic potential of resveratrol: the in vivo evidence. Nat Rev Drug Discov 5:493–506
Challam M, Roy B, Tandon V (2012) In vitro anthelmintic efficacy of Carex baccans (Cyperaceae): ultrastructural, histochemical and biochemical alterations in the cestode, Raillietina echinobothrida. J Parasit Dis 36:81–86
Cox FE (2002) History of human parasitology. Clin Microbiol Rev 15(4):595–612
CPCSEA (1998) Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) guidelines for laboratory animal facility published in the gazette of India
Dixon BR, Arai HP (1991) Anthelmintic-induced destrobilation and its influence on calculated drug efficacy in Hymenolepis diminuta infections in rats. J Parasitol 77:769–774
Giri BR, Roy B (2014) Resveratrol induced structural and biochemical alterations in the tegument of Raillietina echinobothrida. Parasitol Int 63(2):432–437
Giri BR, Roy B, Sinha Babu SP (2013) Evidence of apoptosis in Raillietina echinobothrida induced by methanolic extracts of three traditional medicinal plants of Northeast India. Exp Parasitol 134(4):466–473
González-Sarrías A, Gromek S, Niesen D, Seeram NP, Henry GE (2011) Resveratrol oligomers isolated from Carex species inhibit growth of human colon tumorigenic cells mediated by cell cycle arrest. J Agri Food Chem 59(16):8632–8638
Hu Y, Georghiou SB, Kelleher AJ, Aroian RV (2010) Bacillus thuringiensis Cry5B protein is highly efficacious as a single-dose therapy against an intestinal roundworm infection in mice. PLoS Negl Trop Dis 4(3):e614
Iqbal Z, Lateef M, Jabbar A, Akhtar MS, Khan MN (2006) Anthelmintic activity of Vernonia anthelmintica seeds against trichostrongylid nematodes of sheep. Pharma Biol 44:563–567
Ozkoc S, Tuncay S, Delibas SB, Akisu C (2009) In vitro effects of resveratrol on Trichinella spiralis. Parasitol Res 105(4):1139–1143
Sapaat A, Satrija F, Mahsol HH, Ahmad AH (2012) Anthelmintic activity of papaya seeds on Hymenolepis diminuta infections in rats. Trop Biomed 29(4):508–512
Tritten L, Nwosu U, Vargas M, Keiser J (2012) In vitro and in vivo efficacy of tribendimidine and its metabolites alone and in combination against the hookworms Heligmosomoides bakeri and Ancylostoma ceylanicum. Acta Trop 122(1):101–107
WHO (2002) WHO traditional medicine strategy 2002–2005. WHO/EDM/TRM/2002.1
Williams LD, Burdock GA, Edwards JA, Beck M, Bausch J (2009) Safety studies conducted on high-purity trans-resveratrol in experimental animals. Food Chem Toxicol 49:2170–8221
Xue J, Xiao SH, Xu LL, Qiang HQ (2010) The effect of tribendimidine and its metabolites against Necator americanus in golden hamsters and Nippostrongylus braziliensis in rats. Parasitol Res 106:775–781
Yadav AK, Tangpu V (2008) Anticestodal activity of Adhatoda vasica extracts against Hymenolepis diminuta infections in rats. J Ethnopharmacol 119(2):322–324
Yadav AK, Tengjongmongla (2012) In vivo anthelmintic activity of Clerodendrum colebrookianum Walp., a traditionally used taenicidal plant in Northeast India. Parasitol Res 111(4):1841–1846
Acknowledgments
The authors would like to thank the University Grant Commission for infrastructural support (SAP III) to the Department of Zoology, North-Eastern Hill University, Shillong, and Doctoral Research fellowship (UGC-BSR) to BRG.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Giri, B.R., Bharti, R.R. & Roy, B. In vivo anthelmintic activity of Carex baccans and its active principle resveratrol against Hymenolepis diminuta . Parasitol Res 114, 785–788 (2015). https://doi.org/10.1007/s00436-014-4293-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-014-4293-y