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Sarcocystis arieticanis (Apicomplexa: Sarcocystidae) infecting the heart muscles of the domestic sheep, Ovis aries (Artiodactyla: Bovidae), from K. S. A. on the basis of light and electron microscopic data

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Abstract

In the present study, the heteroxenous life cycle of Sarcocystis species from three strains of the slaughtered sheep at Al-Azizia and Al-Saada abattoirs in Riyadh city, K.S.A., was studied. Muscle samples of the oesophagus, diaphragm, tongue, skeletal and heart muscles were examined. Varied natural infection rates in the muscles of the examined sheep strains were recorded as 83 % in Niemy, 81.5 % in Najdy and 90 % in Sawakny sheep. Muscles of the diaphragm showed the highest infection level above all organs except Najdy sheep in which oesophagus has the highest rate. Also, the heart was the lowest infected organ (40 % Niemy, 44 % Najdy and 53 % Sawakny). Microscopic sarcocysts of Sarcocystis arieticanis are easily identified in sections through the heart muscles of the domestic sheep Ovis aries (Artiodactyla: Bovidae). Cysts measured 38.5–64.4 μm (averaged 42.66 μm) in width and 62.4–173.6 μm (averaged 82.14 μm) in length. The validity of this species was confirmed by means of ultrastructural characteristics of the primary cyst wall (0.1–0.27 μm thick) which revealed the presence of irregularly shaped crowded and hairy-like projections underlined by a thin layer of ground substance. This layer consisted mainly of fine, dense homogenous granules enclosing the developing metrocytes and merozoites that usually contain nearly all the structures of the apical complex and fill the interior cavity of the cyst. Several septa derived from the ground substance divided the cyst into compartments. The merozoites were banana-shaped and measured 12–16 μm in length with centrally or posteriorly located nuclei. Experimental infection of carnivores by feeding heavily infected sheep muscles revealed that the dog, Canis familiaris, is the only final host of the present Sarcocystis species. Gamogony, sporogonic stages and characteristics of sporulated oocysts were also investigated.

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Acknowledgments

The authors extend appreciations to the Deanship of Scientific Research at King Saud University for funding the work through the international research group project IRG14-23 and to the Faculty of Science, Cairo University, Cairo, Egypt.

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Authors and Affiliations

  1. Zoology Department, College of Science, King Saud University, Riyadh, Saudi Arabia

    Saleh Al Quraishy

  2. Zoology department, Faculty of Science, Cairo University, Cairo, Egypt

    Kareem Morsy, Abdel-Rahman Bashtar & Fathy Abdel Ghaffar

  3. Parasitology Institute, Düsseldorf University, Düsseldorf, Germany

    Heinz Mehlhorn

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  1. Saleh Al Quraishy
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  2. Kareem Morsy
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  3. Abdel-Rahman Bashtar
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  4. Fathy Abdel Ghaffar
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  5. Heinz Mehlhorn
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Correspondence to Kareem Morsy.

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Al Quraishy, S., Morsy, K., Bashtar, AR. et al. Sarcocystis arieticanis (Apicomplexa: Sarcocystidae) infecting the heart muscles of the domestic sheep, Ovis aries (Artiodactyla: Bovidae), from K. S. A. on the basis of light and electron microscopic data. Parasitol Res 113, 3823–3831 (2014). https://doi.org/10.1007/s00436-014-4050-2

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