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Comparative analysis of the diagnostic performance of adult, cercarial and egg antigens assessed by ELISA, in the diagnosis of chronic human Schistosoma mansoni infection

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Abstract

Total IgG ELISA was assessed comparing soluble adult worm (SWA), cercarial (SCA), and egg antigens (SEA) using sera of chronic schistosomiasis patients in two different concentrations (1/50 and 1/100). This response showed reactivity against all antigens. Concerning 1/100 serum concentration the SWA gave the best sensitivity (100 %) followed by SCA (86.6 %) and SEA (80 %). The best specificity was obtained from SCA (94.11 %) with an equivalent result from the SWA and SEA (76.47 %). Concerning the 1/50 serum concentration, the SWA gave the best sensitivity (100 %) with an equivalent result from the SCA and SEA (80 %). The best specificity was obtained from SEA (82.35 %) followed by SCA (76.47 %) then SWA (64.7 %). The best AUC value (0.992) was that for SWA indicating its high ability to predict the disease in comparison to (0.914) for SCA and 0.871 for SEA in serum dilution 1/100. The highest AUC value (0.955) was that for SWA in comparison to (0.914) for SEA and (0.88) for SCA in serum dilution 1/50. Comparison between the AUCs from the three mentioned antigens revealed highly significant differences when the data were analyzed by ROC. Each antigen showed a highly statistically significant correlation within its optical density values at both concentrations; 1/100 and 1/50. Comparing the two concentrations in each antigen revealed a non significant correlation obtained from the SWA which signifies a near outcome from both concentrations while the SCA and SEA showed a highly significant difference between the two concentrations. In conclusion, for the diagnosis of chronic schistosomiasis mansoni, total IgG reactivity revealed the best sensitivity by SWA. There was a difference in the type of antigen showing best specificity results between SCA and SEA according to serum concentration used.

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  1. Department of Medical Parasitology, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Rania Mohammad Sarhan, Heba AbdelKader Aminou & Ghada Abdel Rahman Saad

  2. Internal Medicine Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt

    Ossama Ashraf Ahmed

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  1. Rania Mohammad Sarhan
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Sarhan, R.M., Aminou, H.A., Saad, G.A.R. et al. Comparative analysis of the diagnostic performance of adult, cercarial and egg antigens assessed by ELISA, in the diagnosis of chronic human Schistosoma mansoni infection. Parasitol Res 113, 3467–3476 (2014). https://doi.org/10.1007/s00436-014-4017-3

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