Skip to main content

Advertisement

SpringerLink
Log in

Longevity of Sm-p80-specific antibody responses following vaccination with Sm-p80 vaccine in mice and baboons and transplacental transfer of Sm-p80-specific antibodies in a baboon

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Based on data obtained using vaccine efficacy studies in mice, hamsters, and baboons, the credentials of Sm-p80 as a first tier vaccine candidate for schistosomiasis have been well established. Sm-p80-based vaccine formulation(s) have consistently exhibited potent prophylactic efficacy in reducing adult worm burden following cercarial challenge and induce killing of established adult worms in chronic infection. This vaccine is protective against both intestinal and urinary schistosomiasis. In this study, the longevity of Sm-p80-specific antibody responses was studied in mice and in baboons. Robust antibody titers were detected in mice for up to 60 weeks following vaccination with Sm-p80 recombinant vaccine (Sm-p80 + GLA-SE). In the follow-up experiments to our published studies, Sm-p80-specific IgG was also detected in baboons 5–8 years following the initial vaccination with an Sm-p80 DNA vaccine. In one baboon, transfer of Sm-p80-specific antibody was detected in umbilical cord blood and in the baby. These long-lasting humoral immune response data coupled with the vaccine efficacy data in rodents and nonhuman primates further strengthens the case for Sm-p80 to be moved forward through development leading to human clinical trials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig 6

Similar content being viewed by others

References

  • Ahmad G, Zhang W, Torben W, Ahrorov A, Damian RT, Wolf RF, White GL, Carey DW, Mwinzi PN, Ganley-Leal L, Kennedy RC, Siddiqui AA (2011) Preclinical prophylactic efficacy testing of Sm-p80-based vaccine in a nonhuman primate model of Schistosoma mansoni infection and immunoglobulin G and E responses to Sm-p80 in Human serum samples from an area where schistosomiasis is endemic. J Infect Dis 204:1437–1449

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ahmad G, Zhang W, Torben W, Damian RT, Wolf RF, White GL, Chavez-Suarez M, Kennedy RC, Siddiqui AA (2009a) Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model. Vaccine 27:2830–2837

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Ahmad G, Zhang W, Torben W, Haskins C, Diggs S, Noor Z, Le L, Siddiqui AA (2009b) Prime-boost and recombinant protein vaccination strategies using Sm-p80 protects against Schistosoma mansoni infection in the mouse model to levels previously attainable only by the irradiated cercarial vaccine. Parasitol Res 105:1767–1777

    Article  PubMed Central  PubMed  Google Scholar 

  • Attallah AM, Ghanem GE, Ismail H, El Waseef AM (2003) Placental and oral delivery of Schistosoma mansoni antigen from infected mothers to their newborns and children. Am J Trop Med Hyg 68:647–651

    PubMed  Google Scholar 

  • Beaumier CM, Gillespie PM, Hotez PJ, Bottazzi ME (2013) New vaccines for neglected parasitic diseases and dengue. Transl Res 162:144–155

    Article  CAS  PubMed  Google Scholar 

  • Bergquist NR, Leonardo LR, Mitchell GF (2005) Vaccine-linked chemotherapy: can schistosomiasis control benefit from an integrated approach? Trends Parasitol 21:112–117

    Article  CAS  PubMed  Google Scholar 

  • Bergquist R, Al-Sherbiny M, Barakat R, Olds R (2002) Blueprint for schistosomiasis vaccine development. Acta Trop 82:183–192

    Article  PubMed  Google Scholar 

  • Carter D, Reed SG (2010) Role of adjuvants in modeling the immune response. Curr Opin HIV AIDS 5:409–413

    Article  PubMed Central  PubMed  Google Scholar 

  • Clark EH, Silva CJ, Weiss GE, Li S, Padilla C, Crompton PD, Hernandez JN, Branch OH (2012) Plasmodium falciparum malaria in the Peruvian Amazon, a region of low transmission, is associated with immunologic memory. Infect Immun 80:1583–1592

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Elgueta R, de Vries VC, Noelle RJ (2010) The immortality of humoral immunity. Immunol Rev 236:139–150

    Article  CAS  PubMed  Google Scholar 

  • Gabriel S, De BJ, Phiri IK, Masuku M, Riveau G, Schacht AM, Deelder AM, Van Dam GJ, Vercruysse J (2002) Transplacental transfer of schistosomal circulating anodic antigens in cows. Parasite Immunol 24:521–525

    Article  CAS  PubMed  Google Scholar 

  • Gabriel S, Geldhof P, Phiri IK, Cornillie P, Goddeeris BM, Vercruysse J (2005) Placental transfer of immunoglobulins in cattle infected with Schistosoma mattheei. Vet Immunol Immunopathol 104:265–272

    Article  CAS  PubMed  Google Scholar 

  • Hotez PJ, Kamath A (2009) Neglected tropical diseases in sub-saharan Africa: review of their prevalence, distribution, and disease burden. PLoS Negl Trop Dis 3:e412

    Article  PubMed Central  PubMed  Google Scholar 

  • Ireton GC, Reed SG (2013) Adjuvants containing natural and synthetic Toll-like receptor 4 ligands. Expert Rev Vaccines 12:793–807

    Article  CAS  PubMed  Google Scholar 

  • Jenkins-Holick DS, Kaul TL (2013) Schistosomiasis. Urol Nurs 33:163–170

    PubMed  Google Scholar 

  • Karmakar S, Zhang W, Ahmad G, Torben W, Alam MU, Le L, Damian RT, Wolf RF, White GL, Carey DW, Carter D, Reed SG, Siddiqui AA (2014a) Cross-species protection: Schistosoma mansoni Sm-p80 vaccine confers protection against Schistosoma haematobium in hamsters and baboons. Vaccine 32:1296–1303

  • Karmakar S, Zhang W, Ahmad G, Torben W, Alam MU, Le L, Damian RT, Wolf RF, White GL, Carey DW, Carter D, Reed SG, Siddiqui AA (2014b) Use of an Sm-p80–based therapeutic vaccine to kill established adult schistosome parasites in chronically infected baboons. J Infect Dis. doi:10.1093/infdis/jiu031

  • King CH (2010) Parasites and poverty: the case of schistosomiasis. Acta Trop 113:95–104

    Article  PubMed Central  PubMed  Google Scholar 

  • Kitphati R, Pooruk P, Lerdsamran H, Poosuwan S, Louisirirotchanakul S, Auewarakul P, Chokphaibulkit K, Noisumdaeng P, Sawanpanyalert P, Puthavathana P (2009) Kinetics and longevity of antibody response to influenza A H5N1 virus infection in humans. Clin Vaccine Immunol 16:978–981

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Komegae EN, Grund LZ, Lopes-Ferreira M, Lima C (2013a) The longevity of Th2 humoral response induced by proteases natterins requires the participation of long-lasting innate-like B cells and plasma cells in spleen. PLoS One 8:e67135

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Komegae EN, Grund LZ, Lopes-Ferreira M, Lima C (2013b) TLR2, TLR4 and the MyD88 signaling are crucial for the in vivo generation and the longevity of long-lived antibody-secreting cells. PLoS One 8:e71185

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • McManus DP, Loukas A (2008) Current status of vaccines for schistosomiasis. Clin Microbiol Rev 21:225–242

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Mo AX, Agosti JM, Walson JL, Hall BF, Gordon L (2014) Schistosomiasis elimination strategies and potential role of a vaccine in achieving global health goals. Am J Trop Med Hyg 90:54–60

    Article  PubMed  Google Scholar 

  • Novato-Silva E, Gazzinelli G, Colley DG (1992) Immune responses during human Schistosomiasis mansoni. XVIII. Immunologic status of pregnant women and their neonates. Scand J Immunol 35:429–437

    Article  CAS  PubMed  Google Scholar 

  • Palmeira P, Costa-Carvalho BT, Arslanian C, Pontes GN, Nagao AT, Carneiro-Sampaio MM (2009) Transfer of antibodies across the placenta and in breast milk from mothers on intravenous immunoglobulin. Pediatr Allergy Immunol 20:528–535

    Article  PubMed  Google Scholar 

  • Parker M, Allen T (2011) Does mass drug administration for the integrated treatment of neglected tropical diseases really work? Assessing evidence for the control of schistosomiasis and soil-transmitted helminths in Uganda. Health Res Policy Syst 9:3

    Article  PubMed Central  PubMed  Google Scholar 

  • Peroni DG, Chirumbolo S, Veneri D, Piacentini GL, Tenero L, Vella A, Ortolani R, Raffaelli R, Boner AL (2013) Colostrum-derived B and T cells as an extra-lymphoid compartment of effector cell populations in humans. J Matern Fetal Neonatal Med 26:137–142

    Article  CAS  PubMed  Google Scholar 

  • Prichard RK, Basanez MG, Boatin BA, McCarthy JS, Garcia HH, Yang GJ, Sripa B, Lustigman S (2012) A research agenda for helminth diseases of humans: intervention for control and elimination. PLoS Negl Trop Dis 6:e1549

    Article  PubMed Central  PubMed  Google Scholar 

  • Reed SG, Orr MT, Fox CB (2013) Key roles of adjuvants in modern vaccines. Nat Med 19:1597–1608

    Article  CAS  PubMed  Google Scholar 

  • Rollinson D, Knopp S, Levitz S, Stothard JR, Tchuem Tchuente LA, Garba A, Mohammed KA, Schur N, Person B, Colley DG, Utzinger J (2013) Time to set the agenda for schistosomiasis elimination. Acta Trop 128:423–440

    Article  PubMed  Google Scholar 

  • Sabarth N, Savidis-Dacho H, Schwendinger MG, Bruhl P, Portsmouth D, Crowe BA, Kistner O, Barrett PN, Kreil TR, Howard MK (2012) A cell culture-derived whole-virus H5N1 vaccine induces long-lasting cross-clade protective immunity in mice which is augmented by a homologous or heterologous booster vaccination. Vaccine 30:5533–5540

    Article  CAS  PubMed  Google Scholar 

  • Siddiqui AA, Pinkston JR, Quinlin ML, Saeed Q, White GL, Shearer MH, Kennedy RC (2005) Characterization of the immune response to DNA vaccination strategies for schistosomiasis candidate antigen, Sm-p80 in the baboon. Vaccine 23:1451–1456

    Article  CAS  PubMed  Google Scholar 

  • Skerry CM, Mahon BP (2011) A live, attenuated Bordetella pertussis vaccine provides long-term protection against virulent challenge in a murine model. Clin Vaccine Immunol 18:187–193

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Steinhagen F, Kinjo T, Bode C, Klinman DM (2011) TLR-based immune adjuvants. Vaccine 29:3341–3355

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Taillardet M, Haffar G, Mondiere P, Asensio MJ, Pleau-Pison T, Burdin N, Defrance T, Genestier L (2010) Toll-like receptor agonists allow generation of long-lasting antipneumococcal humoral immunity in response to a plain polysaccharidic vaccine. J Infect Dis 202:470–479

    Article  CAS  PubMed  Google Scholar 

  • Terer CC, Bustinduy AL, Magtanong RV, Muhoho N, Mungai PL, Muchiri EM, Kitron U, King CH, Mutuku FM (2013) Evaluation of the health-related quality of life of children in Schistosoma haematobium-endemic Communities in Kenya: a cross-sectional study. PLoS Negl Trop Dis 7:e2106

    Article  PubMed Central  PubMed  Google Scholar 

  • Torben W, Ahmad G, Zhang W, Nash S, Le L, Karmakar S, Siddiqui AA (2012) Role of antibody dependent cell mediated cytotoxicity (ADCC) in Sm-p80-mediated protection against Schistosoma mansoni. Vaccine 30:6753–6758

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Torben W, Ahmad G, Zhang W, Siddiqui AA (2011) Role of antibodies in Sm-p80-mediated protection against Schistosoma mansoni challenge infection in murine and nonhuman primate models. Vaccine 29:2262–2271

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Vujanic A, Snibson KJ, Wee JL, Edwards SJ, Pearse MJ, Scheerlinck JP, Sutton P (2012) Long-term antibody and immune memory response induced by pulmonary delivery of the influenza Iscomatrix vaccine. Clin Vaccine Immunol 19:79–83

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Zhang W, Ahmad G, Torben W, Noor Z, Le L, Damian RT, Wolf RF, White GL, Chavez-Suarez M, Podesta RB, Kennedy RC, Siddiqui AA (2010) Sm-p80-based DNA vaccine provides baboons with levels of protection against Schistosoma mansoni infection comparable to those achieved by the irradiated cercarial vaccine. J Infect Dis 201:1105–1112

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This work is supported by a grant from the NIAID/NIH (R01AI071223) to Afzal A. Siddiqui; NIH grants (P40RR012317, P40OD010431 and P40OD010988) to Gary L. White and Roman F. Wolf; SBIR/NIH grant (1R43AI103983) to Darrick Carter and Afzal A. Siddiqui; Bill and Melinda Gates Foundation grant (OPP1055855) to Steven G. Reed and Darrick Carter. Snails were provided by the Schistosome Research Reagent Resource Center for distribution by BEI Resources, NIAID, NIH: Schistosoma mansoni, strain NMRI exposed Biomphalaria glabrata, strain NMRI, NR-21962.

Author information

Authors and Affiliations

  1. Center for Tropical Medicine and Infectious Diseases, Texas Tech University Health Sciences Center, 3601 4th Street, Mail Stop 6591, Lubbock, TX, 79430, USA

    Weidong Zhang, Loc Le, Juan U. Rojo, Souvik Karmakar, Kory A. Tillery & Afzal A. Siddiqui

  2. Department of Immunology and Molecular Microbiology, Texas Tech University Health Sciences Center, Lubbock, TX, 79430, USA

    Weidong Zhang, Loc Le, Juan U. Rojo, Souvik Karmakar, Kory A. Tillery & Afzal A. Siddiqui

  3. Department of Natural Sciences, School of Arts & Sciences, Peru State College, Peru, NE, 68421, USA

    Gul Ahmad

  4. Tulane National Primate Research Center, Covington, LA, 70433, USA

    Workineh Torben

  5. Department of Cellular Biology, University of Georgia, Athens, GA, 30602, USA

    Raymond T. Damian

  6. Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA

    Roman F. Wolf, Gary L. White & David W. Carey

  7. Infectious Disease Research Institute, Seattle, WA, 98102, USA

    Darrick Carter & Steven G. Reed

  8. PAI Life Sciences, Seattle, WA, 98102, USA

    Darrick Carter

  9. Department of Internal Medicine, Texas Tech University Health Sciences Center, Lubbock, TX, 79430, USA

    Afzal A. Siddiqui

  10. Department of Pathology, Texas Tech University Health Sciences Center, Lubbock, TX, 79430, USA

    Afzal A. Siddiqui

Authors
  1. Weidong Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Gul Ahmad
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Loc Le
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Juan U. Rojo
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Souvik Karmakar
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Kory A. Tillery
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Workineh Torben
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Raymond T. Damian
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Roman F. Wolf
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Gary L. White
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. David W. Carey
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Darrick Carter
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Steven G. Reed
    View author publications

    You can also search for this author in PubMed Google Scholar

  14. Afzal A. Siddiqui
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Afzal A. Siddiqui.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, W., Ahmad, G., Le, L. et al. Longevity of Sm-p80-specific antibody responses following vaccination with Sm-p80 vaccine in mice and baboons and transplacental transfer of Sm-p80-specific antibodies in a baboon. Parasitol Res 113, 2239–2250 (2014). https://doi.org/10.1007/s00436-014-3879-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-014-3879-8

Keywords

Search

Navigation