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Chemical characterization and acaricide potential of essential oil from aerial parts of Tagetes patula L. (Asteraceae) against engorged adult females of Rhipicephalus sanguineus (Latreille, 1806)

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Abstract

Rhipicephalus sanguineus, commonly known as the brown dog tick, is one of the most widely distributed species of tick. In dogs, it can cause anemia and provide the transmission of pathogenic microorganisms such as Babesia canis, Ehrlichia canis, Hepatozoon canis, Anaplasma platys, and Mycoplasma haemocanis. To man, it can transmit the intracellular parasites Rickettsia rickettsii and Rickettsia conorii, the causative agents of the Rocky Mountain spotted fever in the Americas and Mediterranean and spotted fever in Europe and North Africa. Its control is performed by applying synthetic formulations composed of pyrethroids; however, continued use of these products results in environmental damage and acquisition of resistance. Alternatively, studies with botanical insecticides have been increasingly recurrent. Therefore, this study aimed to test the efficacy of essential oil of Tagetes patula, a ruderal species widely described in the literature for its insecticidal properties, in engorged females of R. sanguineus by the adults immersion test (AIT) and impregnated paper disk test (IPDT). The essential oil used, through gas chromatography coupled to mass spectrometry, revealed the presence of 55 compounds, being the 4-vinyl guaiacol and gamma terpinene the majority ones. The AIT compared to the IPDT was more efficient in inhibiting oviposition of tick; however, the eggs laid by the females submitted to saturated atmosphere with essential oil, from IPDT, not hatched, interrupted their development cycle. Besides being a pioneer work, the results presented here contributes to new researches, aiming the incorporation of essential oil in an acaricide for use in the environment.

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References

  • Almonsny NR, Massard CL (1999) Erliquiose felina - Revisão. Clín Vet 23:30–32

    Google Scholar 

  • Chalcat LC, Garry RP, Muhayimana A (1995) Essential oil of Tagetes minuta from Rwanda and France: chemical composition according to harvesting location, growth stage and part of plant extracted. J Essent Oil Res 7:375–386

    Article  Google Scholar 

  • Chamorro ER, Sequeira AF, Velasco GA, Zalazar MF, Ballerini GA (2011) Evaluation of Tagetes minuta L. essential oils control Varroa destructor (Acari: Varroidae). J Argent Chem Soc 98:39–47

    CAS  Google Scholar 

  • Dantas-Torres F, Figueredo LA, Brandão-Filho SP (2006) Rhipicephalus sanguineus (Acari: Ixodidae), the brown dog tick, parasitizing humans in Brazil. Rev Soc Bras Med Trop 39(1):64–67

    Article  PubMed  Google Scholar 

  • Dharmaggada VSS, Naik SN, Mittal PK, Vasudevan P (2005) Larvicidal activity of Tagetes patula essential oil against three mosquito species. Biores Technol 96:1235–1240

    Article  Google Scholar 

  • Dipeolu OO (1984) Studies on ticks of veterinary importance in Nigeria. XVI. The oviposition pattern of engorged Boophilus and Hyalomma species when subjected in the laboratory to artificially created factors. Acarologia 25(3):231–240

    Google Scholar 

  • Faizi S, Fayyaz S, Bano S, Igbal EY, Lubna SH, Naz H (2011) Isolation of nematicidal compounds from Tagetes patula L. yellow flowers: structure-activity relationship studies against cyst nematode Heterodera zeae infective stage larvae. J Agric Food Chem 59(17):9080–9093

    Article  PubMed  CAS  Google Scholar 

  • Gillij YG, Gleiser RM, Zygadlo JA (2008) Mosquito repellent activity of essential oils of aromatic plants growing in Argentina. Bioresour Technol 99:2507–2515

    Article  PubMed  CAS  Google Scholar 

  • Guimarães JH, Tucci EC, Barros-Battesti DM (2001) Ectoparasitos de importância veterinária. Plêidade, São Paulo

    Google Scholar 

  • Héthélyi E, Danos B, Tétényi P, Koczka I (1986) GC-MS Analysis of the essential oils of four Tagetes species and the anti-microbial activity of Tagetes minuta. Flavour Frag J 1:169–173

    Article  Google Scholar 

  • Labarthe NV (1994) Biological control of tick populations: review and reflections. Cad Saúde Públ 10(1):47–52

    Article  CAS  Google Scholar 

  • Lawrence BM (1985) A review of the world production of essential oil. Perfum Flavor 10:2–16

    Google Scholar 

  • Lees AD, Beament JW (1948) An egg-waxing organ in ticks. Q J Microsc Sci 89(3):291–322

    PubMed  CAS  Google Scholar 

  • Lemos ERS, Rozental T, Villela LC (2002) Brazilian spotted fever: description of a fatal clinical case in the State of Rio de Janeiro. Rev Soc Bras Med Trop 35:523–525

    Article  PubMed  Google Scholar 

  • Mantovani E, Costa IP, Gauditano G, Bonoldi VLN, Higuchi ML, Yoshinari NH (2007) Description of Lyme disease-like syndrome in Brazil. Is it a new tick borne disease or Lyme disease variation? Braz J Med Biolog Res 40(4):443–456

    Article  CAS  Google Scholar 

  • Marotti M, Piccaglia R, Giovanelli E, Deans SG, Eaglesham E (1994) Effects of planting time and mineral fertilization on peppermint (Mentha x piperita L.) essential oil composition and its biological activity. Flav Fragr J 9(3):125–129

    Article  CAS  Google Scholar 

  • Massera PE, Rodriguez T, Giulietti AM (1998) Thiophene production in transformed roots cultures of Tagetes filifolia. Biotec Lett 20(6):573–577

    Article  CAS  Google Scholar 

  • Meshkatalsadat MH, Safaei-Ghomi J, Moharramipour S, Nasseri M (2010) Chemical characterization of volatile components of Tagetes minuta L. cultivated in south west of Iran by nano scale injection. Dig J Nanomater Biostruct 5(1):101–106

    Google Scholar 

  • Miller RJ, George JE, Guerrero F, Carpenter L, Welch JB (2001) Characterization of acaricide resistance in Rhipicephalus sanguineus (Latreille) (Acari: Ixodidae) collected from the Corozal Army Veterinary Quarantine Center, Panama. J Med Entomol 38:298–302

    Article  PubMed  CAS  Google Scholar 

  • O'Dwyer LH, Massard CL (2001) Aspectos gerais da hepatozoonose canina. Rev Clín Vet 31:34–40

    Google Scholar 

  • Pegram RG, Keirans JE, Clifford CM, Walker JB (1987) Clarification of the Rhipicephalus sanguineus group (Acari, Ixodoidea, Ixodidae). II. R. sanguineus (Latreille, 1806) and related species. Syst Parasitol 10:27–44

    Article  Google Scholar 

  • Politi FAS, Figueira GM, Araújo AM, Sampieri BR, Camargo-Mathias MI, Szabó MJP, Bechara GH, Santos LC, Vilegas W, Pietro RCLR (2012) Acaricidal activity of ethanolic extract from aerial parts of Tagetes patula L. (Asteraceae) against larvae and engorged adult females of Rhipicephalus sanguineus (Latreille, 1806). Paras Vect 5:295

    Article  Google Scholar 

  • Restello RM, Menegatt C, Mossi AJ (2009) Effect of the essential oil of Tagetes patula L. (Asteraceae) on Sitophilus zeamais Motschulsky (Coleoptera, Curculionidae). Rev Bras Entomol 53(2):304–307

    Article  Google Scholar 

  • Ribeiro VLS, Rolim V, Bordignon S, Henriques AT, Dorneles GG, Limberger RP, Von Poser G (2008a) Chemical composition and larvicidal properties of the essential oils from Drimys brasiliensis Miers (Winteraceae) on the cattle tick Rhipicephalus (Boophilus)microplus and the brown dog tickRhipicephalus sanguineus. Parasitol Res 102(3):531–535

    Article  PubMed  Google Scholar 

  • Ribeiro VLS, Avancini C, Gonçalves K, Toigo E, Von Poser G (2008b) Acaricidal activity of Calea serrata (Asteraceae) on Boophilus microplus and Rhipicephalus sanguineus. Vet Parasitol 151:351–354

    Article  PubMed  Google Scholar 

  • Romagnoli C, Bruni R, Andreotti E, Rai MK, Viventi CB, Mares D (2005) Chemical characterization and antifungal activity of essential oil of capitula from wild Indian Tagetes patula L. Protoplasma 225:57–65

    Article  PubMed  CAS  Google Scholar 

  • Rondón M, Velasco J, Hernandéz J, Pecheneda M, Rojas J, Morales A, Carmona J, Díaz T (2006) Chemical composition and antibacterial activity of the essential oil of Tagetes patula L. (Asterarcea) collected from the Venezuela Andes. Rev Latinoamer Quím 34:1–3

    Google Scholar 

  • Sabatini GA, Kemp DH, Hughes S, Nari A, Hansen J (2001) Tests to determine LC50 and discriminating doses for macrocyclic lactones against the cattle tick, Boophilus microplus. Vet Parasitol 95:53–62

    Article  PubMed  CAS  Google Scholar 

  • Sampieri BR, Furquim KCS, Nunes PH, Camargo-Mathias MI (2013) Rhipicephalus sanguineus (Acari: Ixodidae) female ticks exposed to castor oil (Ricinus communis): an ultrastructural overview. Parasitol Res 112(2):611–619

    Article  PubMed  CAS  Google Scholar 

  • Semmler M, Abdel-Ghaffar F, Al-Rasheid K, Mehlhorn H (2009) Nature helps: from research to products against blood-sucking arthropods. Parasitol Res 105(6):1483–1487

    Article  PubMed  Google Scholar 

  • Stojanova A, Paraskevova P, Anastassov CH (2000) A comparative investigation on the essential oil composition of two Bulgarian cultivars of Mentha piperita L. J Essent Oil Res 12:438–440

    Article  CAS  Google Scholar 

  • Swango LJ, Bankemper KW, Kong LI (1992) Infecções bacterianas, riquetsiais, protozoais e outras. In: Ettinger SJ (ed) Tratado de medicina veterinária interna: moléstias do cão e do gato, 3rd edn. Manole, São Paulo, pp 277–311

    Google Scholar 

  • Szarka S, Héthelyi E, Lemberkoviks E, Kuzovkina IN, Banyai P, Szöke E (2006) GC and GC-MS studies on the essential oil and thiophenes from Tagetes patula L. Chromatographia 63:67–73

    Article  Google Scholar 

  • Szarka S, Héthelyi E, Kuzovkina IN, Lemberkovics E, Szöke E (2008) GC–MS Method Development for the analyses of thiophenes from solvent extracts of Tagetes patula L. Chromatographia 68(1):63–69

    Article  Google Scholar 

  • Thompson C, Spielman A, Krause PJ (2001) Coinfecting deer-associated zoonoses: lyme disease, Babesiosis, and Ehrlichiosis. Clin Infect Dis 33:676–685

    Article  PubMed  CAS  Google Scholar 

  • Toloza AC, Zygadlo J, Mougabure CG, Biurrun F, Zerba E, Picollo MI (2006) Fumigant and repellent properties of essential oils and component compounds against permethrin-resistant Pediculus humanus capitis (Anoplura: Pediculidae) from Argentina. J Med Entomol 43:889–895

    Article  PubMed  CAS  Google Scholar 

  • Trapp SM, Messick JB, Vidotto O, Jojima FS, Morais HSA (2006) Babesia gibsoni genotype Asia in dogs from Brazil. Vet Parasitol 141(1–2):177–180

    Article  PubMed  Google Scholar 

  • Vázquez AM, Demmel GI, Criado SG, Aimar ML, Cantero JJ, Rossi LI, Velasco MI (2011) Phytochemistry of Tagetes minuta L. (Asteraceae) from Córdoba, Argentina: comparative study between essential oil and HS-SPME analyses. Bol Latinoam Caribe Plant Med Aromat 10(4):351–362

    Google Scholar 

  • Vieira LS, Cavalcante ACR, Pereira MF, Dantas LB, Ximenes LJF (1999) Evaluation of anthelmintic efficacy of plants available in Ceará State, North-East Brazil, for the control of goats gastrointestinal nematodes. Rev Med Vet 150(5):447–452

    Google Scholar 

  • Zygadlo JA, Grosso NR, Guzman CA (1994) Antifungal properties of the leaf oils of Tagetes minuta L. and Tagetes filifolia Lag. J Essent Oil Res 6:617–621

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank the São Paulo Research Foundation (FAPESP) for financial support and to Professor Didier Raoult by the interinstitutional agreement.

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Authors and Affiliations

  1. Department of Drugs and Medicines, School of Pharmaceutical Sciences, UNESP—Univ Estadual Paulista, Rodovia Araraquara-Jaú, Km 1, 14801-902, Araraquara, Sao Paulo, Brazil

    Flávio Augusto Sanches Politi, Tatiana Maria de Souza-Moreira, Edvânio Ramos Rodrigues, Geisiany Maria de Queiroz & Rosemeire Cristina Linhari Rodrigues Pietro

  2. Chemical, Biological and Agricultural Pluridisciplinary Research Center (CPQBA), UNICAMP—Campinas State University, Sao Paulo, Campinas, 13081-970, Brazil

    Glyn Mara Figueira

  3. Laboratório de Produtos Naturais (GPNUF), Universidade de Franca (UNIFRAN), Av. Dr. Armando Salles Oliveira 201, CP 82, Parque Universitário, Franca, Sao Paulo, Brazil

    Ana Helena Januário

  4. Unité de Recherche Sur Les Maladies Infectieuses et Tropicales Emergentes (URMITE), UMR 6236, Faculté de Médecine, Université de la Méditerranée, Boulevard Jean Moulin 27, 13358, Marseille, Cedex 5, Marseille, France

    Jean-Michel Berenger, Cristina Socolovschi & Philippe Parola

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  1. Flávio Augusto Sanches Politi
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  2. Tatiana Maria de Souza-Moreira
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  4. Geisiany Maria de Queiroz
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  9. Philippe Parola
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  10. Rosemeire Cristina Linhari Rodrigues Pietro
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Correspondence to Rosemeire Cristina Linhari Rodrigues Pietro.

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Politi, F.A.S., de Souza-Moreira, T.M., Rodrigues, E.R. et al. Chemical characterization and acaricide potential of essential oil from aerial parts of Tagetes patula L. (Asteraceae) against engorged adult females of Rhipicephalus sanguineus (Latreille, 1806). Parasitol Res 112, 2261–2268 (2013). https://doi.org/10.1007/s00436-013-3386-3

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