Abstract
Biological control with the use of nematophagous fungi has been described very successfully by many authors and presents itself as a complementary control method, acting on the free-living forms of helminths. The efficacy of a formulation containing the fungus Pochonia chlamydosporia in controlling Fasciola hepatica eggs in faeces was evaluated in an experimental field assay. Two bovine groups (six animals each) were used: A (control) and B (treated with fungus). At 30 days after deworming, the animals were separated into two similar paddocks with flooded areas and were given pellets containing 25 % mycelial mass (group B) or no fungus (group A) at a dose of 1 g/10 kg body weight, twice a week, during 18 months. Faecal samples were harvested fortnightly in the animals of groups A and B and they were submitted at examination of quantitative sedimentation. The mean count of F. hepatica eggs per grams of faeces was significantly higher in group A (1.19) compared with those from group B (0.82) (P < 0.01). After 18 months, animals from group B had gained 42.33 kg above (17.82 % more by weight) (P < 0.01), compared with the control group (A). Every month, faecal samples from paddocks A and B were collected and they were incubated. P. chlamydosporia was identified only in sample source of the paddock B. It can be concluded that the application of this fungical formulation with P. chlamydosporia 25 % mycelial mass was effective in reducing the availability of eggs in the environment and reinfections in calves in natural conditions.
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References
Allen RG, Pereira LS, Raes D, Smith M. Crop (1998) Evapotranspiration—guidelines for computing crop water requirements, FAO Irrigation and drainage paper 56. Water Resources, Development and Management Service, FAO Rome, Italy http://www.fao.org/docrep/X0490E/X0490E00.htm. Accessed 23 June 2009
Amato SB, de Rezende HE, Gomes DC, da Serra Freire NM (1986) Epidemiology of Fasciola hepatica infection in the Paraíba River Valley, São Paulo, Brasil. Vet Parasitol 22:275–284
Araújo JV, Freitas BW, Vieira TC, Campos AK (2006) Avaliação do fungo predador de nematóides Duddingtonia flagrans sobre larvas infectantes de Haemonchus contortus e Strongyloides papillosus de caprinos. Rev Bras Parasitol Vet 15:76–79
Araújo JV, Braga FR, Araújo JM, Silva AR, Tavela AO (2008) In vitro evaluation of the effect of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Ascaris suum eggs. Parasitol Res 102:787–790
Araujo JM, Araújo JV, Braga FR, Carvalho RO, Ferreira SR (2009) Activity of the nematophagous fungi Pochonia chlamydosporia, Duddingtonia flagrans and Monacrosporium thaumasium on egg capsules of Dipylidium caninum. Vet Parasitol 166:86–89
Ayres M, Ayres JRM, Ayres DL, Santos AS (2007) Aplicações estatísticas nas áreas de ciências biológicas, 5th edn. Belém, Pará, 380 pp
Braga FR, Araújo JV, Campos AK, Araújo JM, Carvalho RO, Silva AR, Tavela AO (2008) In vitro evaluation of the action of the nematophagous fungi Duddingtonia flagrans, Monacrosporium sinense and Pochonia chlamydosporia on Fasciola hepatica eggs. World J Microbiol Biotechnol 24:1559–1564
Braga FR, Araújo JV, Silva AR, Carvalho RO, Araújo JM, Ferreira SR, Carvalho GR (2010) Viability of the nematophagous fungus Pochonia chlamydosporia after passage through the gastrointestinal tract of horses. Vet Parasitol 168:264–268
Brennan GP, Fairweather I, Trudgett A, Hoey E, Mccoy M, Mcconville M, Meaney M, Robinson M, Mcferran N, Ryan L, Lanusse C, Mottier L, Alvarez L, Solana H, Virkel G, Brophy PM (2007) Understanding triclabendazole resistance. Exp Mol Pathol 82:104–109
Dalton JP (1999) Fasciolosis, 1st edn. CABI, Oxon, 562 pp
de Araújo SM, Pile EAM, Barros JSL, Santos JAA, Vasconcellos MC (2002) Alterações histológicas em Lymnaea columella provocadas pelo látex de Euphorbia splendens var. hislopii. Braz J Vet Res Anim Sci 39:157–159
Dias AS, Araújo JV, Campos AK, Braga FR, Fonseca TA (2007) Application of a formulation of the nematophagous fungus Duddingtonia flagrans in the control of cattle gastrointestinal nematodiosis. World J Microbiol Biotechnol 38:1245–1252
Dias AS, Araújo JV, Braga FR, Araujo JM, Puppin AC, Fernandes FM, Ramos RF, Bertonceli RM, da Silva RG, Perboni WR (2012) Biological control of Fasciola hepatica eggs with the Pochonia chlamydosporia fungus after passing through the cattle gastrointestinal tract. Parasitol Res 110:663–667
Echevarria F (2004) Fasciolose. Rev Bras Parasitol Vet 13:100–103
Fairweather I (2005) Triclabendazole: new skills to unravel an old(ish) enigma. J Helminthol 79:227–234
Fontenot ME, Miller JE, Peña MT, Larsen M, Gillespie A (2003) Efficiency of feeding Duddingtonia flagrans chlamydospores to grazing ewes on reducing availability of parasitic nematode larvae on pasture. Vet Parasitol 118:203–213
Fox NJ, White PCL, McClean CJ, Marion G, Evans A, Hutching MR (2011) Predicting impacts of climate change on Fasciola hepatica risk. PLoS One 6(1):e16126
Gams W, Zare R (2001) A revision of Verticillium sect. Prostrata. III. Generic classification. Nova Hedwigia 73:329–337
Lackey BA, Muldoon AE, Jaffe BA (1993) Alginate pellet formulation of Hirsutella rossiliensis for biological control of plant-parasitic nematodes. Biol Control 3:155–160
Larsen M, Faedo M, Waller PJ (1994) The potential of nematophagous fungi to control the free-living stages of nematode parasites of sheep: survey for the presence of fungi in fresh faeces of grazing livestock in Australia. Vet Parasitol 53:275–281
Mas-Coma S, Bargues MD, Valero MA (2005) Fascioliasis and other plant-borne trematode zoonoses. Int J Parasitol 35:1255–1278
McKinstry B, Halferty L, Brennan GP, Fairweather I (2009) Morphological response of triclabendazole-susceptible and triclabendazole-resistant isolates of Fasciola hepatica to treatment in vitro with nitroxynil (Trodax). Parasitol Res 104:645–655
Oliveira DR, Ferreira DM, Stival CC, Romero F, Cavagnolli F, Kloss A, Araújo FB, Molento MB (2008) Resistência da Fasciola hepatica ao triclabendazole em ovinos e caprinos durante um surto ocorrido em Almirante Tamandaré, Paraná. Rev Bras Parasitol Vet 17:149–153
Smith WD, Zarlenga DS (2006) Developments and hurdles in generating vaccines for controlling helminth parasites of grazing ruminants. Vet Parasitol 139:347–359
Ueno W, Gonçalves PC (1998) Manual para diagnóstico das helmintoses de ruminantes, 4th edn. Japan International Cooperation Agency, Tokyo, 143 pp
Walker HL, Connick WJ (1983) Sodium alginate for production and formulation of mycoherbicides. Weed Sci 31:333–338
Waller PJ, Schwan O, Ljungstrøm B-L, Rydzik A, Yeates GW (2004) Evaluation of biological control of sheep parasites using Duddingtonia flagrans under commercial farming conditions on the island of Gotland, Sweden. Vet Parasitol 126:299–315
Wood IB, Amaral NK, Bairden K, Duncan JL, Kassai T, Malone JB Jr, Pankavich JA, Reinecke RK, Slocombe O, Taylor SM, Vercruysse J (1995) World Association for the Advancement of Veterinary Parasitology (W.A.A.V.P.) second edition of guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine, ovine, caprine). Vet Parasitol 58:181–213
Acknowledgments
The authors would like to thank Fapemig, CNPq, Capes and Capes/Finep for their financial support and grant concession. We would also like to thank the farmer José Puppin for the supply of animals and for the use of his property.
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Dias, A.S., Araújo, J.V., Braga, F.R. et al. Pochonia chlamydosporia in the biological control of Fasciola hepatica in cattle in Southeastern Brazil. Parasitol Res 112, 2131–2136 (2013). https://doi.org/10.1007/s00436-013-3372-9
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DOI: https://doi.org/10.1007/s00436-013-3372-9