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Some characteristics of IL-5-producing T cells in mouse liver induced by Schistosoma japonicum infection

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Abstract

Schistosome infection could cause significant liver damage in animal; Th2 cells play an important role in the progress of this disease. In our study, C57BL/6 mice were infected by Schistosoma japonicum and lymphocytes were isolated from the liver to detect some characteristics of interleukin-5 (IL-5)-producing T cells by different methods. The results revealed that S. japonicum infection could induce a large amount of IL-5 in mouse liver T cells by the means of fluorescent bead immunoassay and RT-PCR. Although, mouse liver contained many T cell subsets, such as Th cells, Tc cells, NKT cells, and γδ T cells. Fluorescence activated cell sorting results indicated that Th cells were the main source of IL-5 in the T cell population after phorbol 12-myristate 13-acetate and ionomycin stimulation. Moreover, the percentage of IL-5-producing Th cells continued to increase from 4 to 8 weeks after S. japonicum infection, which differed from the changes of IFN-γ+ Th1 cells, IL-4+ Th2 cells, and IL-17A+ Th17 cells during S. japonicum infection. Additionally, cytokines co-expression results demonstrated that 36.2 % of IL-5+ Th cells could express IL-4, and 10 % of it could produce IFN-γ or IL-17A. Collectively, these findings implied that IL-5-producing Th cells posses some properties which differ from other cytokines secreting Th cells.

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Acknowledgments

This work was supported by a grant from the Natural Science Foundation of China (30901353) and Natural Science Foundation of Hunan Province, China (11JJ3103) and Science and Technology Planning Project of Guangzhou City (2011 J22007). We thank Prof. Hu Xuchu in the Department of Parasitology at Zhongshan School of Medicine, Sun Yat-sen University who provide help in animal infection.

Conflict of interest

The authors declare no conflict of interest with this work.

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Authors and Affiliations

  1. Department of Functional Experiment Centre, Guangzhou Medical University, 510182, Guangzhou, China

    Hongyan Xie

  2. Department of Pathogenic Biology and Immunology, Guangzhou Medical University, 510182, Guangzhou, China

    Dianhui Chen, Xueping Luo & Zhiyan Gao

  3. Department of Pathogenic Biology and Immunology, Xiangnan University, 423000, Chenzhou, China

    Huilong Fang

  4. Department of Pathogenic Biology and Immunology, Sino-French Hoffmann Institute, Guangzhou Medical University, 510182, Guangzhou, China

    Jun Huang

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  1. Hongyan Xie
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  2. Dianhui Chen
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  3. Xueping Luo
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  4. Zhiyan Gao
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  5. Huilong Fang
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  6. Jun Huang
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Correspondence to Huilong Fang or Jun Huang.

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Hongyan Xie and Dianhui Chen equally contributed to this work.

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Xie, H., Chen, D., Luo, X. et al. Some characteristics of IL-5-producing T cells in mouse liver induced by Schistosoma japonicum infection. Parasitol Res 112, 1945–1951 (2013). https://doi.org/10.1007/s00436-013-3350-2

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  • DOI: https://doi.org/10.1007/s00436-013-3350-2

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