Abstract
Zoonotic cutaneous leishmaniasis (ZCL) is an important public health challenge in Iran. It is often caused by the protozoan parasite, Leishmania major. This pathogen is principally transmitted by the infectious bites of adult female phlebotomine sand flies which belong to the subgenus Phlebotomus (Phlebotomus) Rondani and Berte (Diptera: Psychodidae). A recent outbreak of clinical ZCL cases in the rural district of Jask, Hormozgan province, southern Iran prompted the identification of sand flies naturally infected with L. major using polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA). A total of 8,123 wild sand flies were caught using sticky paper traps indoors and outdoors in seven villages of Jask from March 2007 to February 2008. About 120 trap-nights per month were carried out during peak seasonal density. Eight sand fly species of two distinct genera were morphologically identified. The most abundant species, Phlebotomus papatasi (≈60 %) and Phlebotomus salehi (17 %), had simultaneous population peaks in May and October. The anthropophilic index of P. papatasi was approximately twice that of P. salehi. PCR reactivity of L. major infections in P. salehi was weaker than those in P. papatasi. This is discussed with regard to their role in the natural transmission cycle of ZCL. This study provided the first PCR and ELISA evidence on P. salehi as a vector of L. major parasites in a new endemic region of ZCL in southeast Iran.
Similar content being viewed by others
References
Aransay AM, Scoulica E, Tselentis Y (2000) Detection and identification of Leishmania DNA within naturally infected sandflies by seminested PCR on minicircle kinetoplastic DNA. Appl Environ Microbiol 66:1933–1938
Ashford RW (2001) Leishmaniasis. In: Service MW (ed) The encyclopedia of arthropod-transmitted infections of man and domesticated animals. CABI Publishing, Wallingford, pp 269–279
Azizi K, Rassi Y, Javadian J, Motazedian MH, Asgari Q, Yaghoobi-Ershadi MR (2008) First detection of Leishmania infantum in Phlebotomus (Larroussius) major (Diptera: Psychodidae) from Iran. J Med Entomol 45:726–731
Azizi K, Fakoorziba MR, Jalali M, Moemenbellah-Fard MD (2012a) First molecular detection of Leishmania major within naturally infected Phlebotomus salehi from a zoonotic cutaneous leishmaniasis focus in southern Iran. Trop Biomed 29:1–8
Azizi K, Moemenbellah-Fard MD, Fakoorziba MR, Kalantari M (2012b) Molecular detection of Leishmania major kDNA within wild rodent hosts in a new focus of zoonotic cutaneous leishmaniasis in south-east Iran. Vector Borne Zoonotic Dis (In press)
Depaquit J, Lienard E, Verzeaux-Griffon A, Ferte H, Bounamous A, Gantier JC, Hanafi HA, Jacobson RL, Maroli M, Moin-Vaziri V, Müller F, Özbel Y, Svobodova M, Volf P, Léger N (2008) Molecular homogeneity in diverse geographical populations of Phlebotomus papatasi (Diptera, Psychodidae) inferred from ND4 mtDNA and ITS2 rDNA epidemiological consequences. Infect Genet Evol 8:159–170
Hamarsheh O (2011) Distribution of Leishmania major zymodemes in relation to populations of Phlebotomus papatasi sand flies. Parasites Vectors 4:9
Hamarsheh O, Presber W, Yaghoobi-Ershadi MR, Amro A, Al-Jawabreh A, Sawalha S, Al-Lahem A, Das ML, Guernaoui S, Seridi N, Dhiman RC, Hashiguchi Y, Ghrab J, Hassan M, Schonian G (2009) Population structure and geographical subdivision of the Leishmania major vector Phlebotomus papatasi as revealed by microsatellite variation. Med Vet Entomol 23:69–77
Jacobson RL (2011) Leishmaniasis in an era of conflict in the Middle East. Vector Borne Zoonotic Dis 11:247–258
Kasiri H, Javadian E, Seyedi-Rashti MA (2000) List of Phlebotominae (Diptera: Psychodidae) of Iran. Bull Soc Pathol Exot 93:129–130
Killick-Kendrick R (1999) The biology and control of phlebotomine sand flies. Clin Dermatol 17:279–289
Lane RP (1993) Sand flies (Phlebotominae). In: Lane RP, Crosskey RW (eds) Medical insects and arachnids. Chapman and Hall, London, pp 78–119
Lewis DJ (1971) Phlebotomid sandflies. Bull Wld Hlth Org 44:535–551
Lewis DJ (1982) A taxonomic review of the genus Phlebotomus (Diptera: Psychodidae). Bull Br Mus Nat Hist (Entomol) 45:121–209
Mesghali A (1965) Phlebotominae (Diptera) of Iran. 3. Studies on sand flies in the areas of Bandar Abbas and Jask (Littoral areas of Hormoz Strait and Sea of Oman). Bull Soc Pathol Exot 58:259–276
Moemenbellah-Fard MD, Kalantari M, Rassi Y, Javadian E (2003) The PCR-based detection of Leishmania major infections in Meriones libycus (Rodentia: Muridae) from southern Iran. Ann Trop Med Parasitol 97:811–816
Molyneux DH, Ashford RW (1983) The biology of Trypanosoma and Leishmania, parasites of man and domestic animals. Taylor and Francis, London, pp 240–243
Parvizi P, Ready PD (2008) Nested PCRs and sequencing of nuclear ITS-rDNA fragments detect three Leishmania species of gerbils in sand flies from Iranian foci of zoonotic cutaneous leishmaniasis. Trop Med Int Hlth 13:1159–1171
Parvizi P, Taherkhani H, Ready PD (2010) Phlebotomus caucasicus and Phlebotomus mongolensis (Diptera: Psychodidae): indistinguishable by the mitochondrial cytochrome b gene in Iran. Bull Entomol Res 100:415–420
Ready PD (2011) Should sand fly taxonomy predict vectorial and ecological traits? J Vect Ecol 36(suppl 1):S17–S22
Reithinger R, Dujardin JC, Louzir H, Pirmez C, Alexander B, Brooker S (2007) Cutaneous leishmaniasis. Lancet Infect Dis 7:581–596
Smart J (1965) A handbook for the identification of insects of medical importance, 4th edn. British Museum (Natural History), London
Volf P, Peckova J (2007) Sand flies and Leishmania: specific versus permissive vectors. Trends Parasitol 23:91–92
Acknowledgments
The authors are deeply indebted to the vice-chancellor for research and technology at the Shiraz University of Medical Sciences (SUMS) for permitting the use of facilities at the university. The technical assistance by Ms. T. Dabaghmanesh in the provision of the regional map is acknowledged and much appreciated. This study was part of the research project number 24/P/15047 dated 27-2-2006, Hormozgan University of Medical Sciences. Their assistance in providing logistics and manpower is very much appreciated. The contents of this publication are the sole responsibility of the authors and do not necessarily reflect the views of the relevant institutions.
Conflict of interest
No competing financial interests exist.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Azizi, K., Abedi, F. & Moemenbellah-Fard, M.D. Identification and frequency distribution of Leishmania (L.) major infections in sand flies from a new endemic ZCL focus in southeast Iran. Parasitol Res 111, 1821–1826 (2012). https://doi.org/10.1007/s00436-012-3029-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00436-012-3029-0