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The study of apoptotic bifunctional effects in relationship between host and parasite in cystic echinococcosis: a new approach to suppression and survival of hydatid cyst

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Abstract

Cystic echinococcosis (hydatidosis) is a zoonotic helminthic disease of human and other intermediated hosts wherein infection is caused by the larval stages of tapeworm Echinococcus granulosus. Growth of the larval stage is formed throughout the internal organs, the liver and lung, causing their destruction. Important pathways are unknown about suppression and survival of cysts in human body. In this study, apoptotic bifunctional effects are evaluated in relationship between host and parasite in cystic echinococcosis. Human lymphocytes were treated with hydatid fluid (HF). After 6 h of exposure, caspase-3 activity was measured by fluorometric assay in the HF-treated lymphocytes and control cells. Also, the expression of Bax (as pro-apoptotic protein) and Bcl-2 (an anti-apoptotic protein) mRNA was assessed by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) after 12 h of exposure. For surveying of apoptosis-inducing ligands TNF-related apoptosis-inducing ligand and Fas-L, germinal layer and accompaniment peripheral tissues as healthy control were separated by scalpel from each cyst in sterile condition, then were assess by semiquantitative RT-PCR method in mRNA expression. Both the ratio of Bax/Bcl-2 mRNA expression and caspase-3 activity were higher in the fertile fluid-treated lymphocytes relative to infertile fluid-treated lymphocytes and control group versus the expression level of apoptosis-inducing ligands having a relatively high level in germinal layer of infertile cyst in comparison to fertile cyst and healthy tissue. Apoptosis of germinal layer of fertile cysts is possibly one of the suppression mechanisms in hydatidosis patients, in contrast to lymphocytes apoptosis by modulator of hydatid fluid, one of the hydatid cyst survival mechanisms.

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Acknowledgments

This study was conducted and financially supported by Mashhad University of Medical Sciences. This article is derived from the master's thesis of the first author (Thesis no. A-247). We thank Dr. Esmaeeli for the statistical consults. In addition, we thank all the staff of Ghaem Hospital Mycoparasitology lab and Bu-Ali Research Center Immunobiochemistry Lab for their cooperation.

Conflicts of interest

The authors declare that there is no conflict of interests.

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Authors and Affiliations

  1. Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

    Adel Spotin

  2. Department of Parasitology and Mycology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Monireh Mokhtari Amir Majdi

  3. Immunobiochemistry Laboratory, Immunology Research Center, Bu-Ali research Institute, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mojtaba Sankian & Abdolreza Varasteh

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  1. Adel Spotin
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  2. Monireh Mokhtari Amir Majdi
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  3. Mojtaba Sankian
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  4. Abdolreza Varasteh
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Correspondence to Adel Spotin.

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Spotin, A., Majdi, M.M.A., Sankian, M. et al. The study of apoptotic bifunctional effects in relationship between host and parasite in cystic echinococcosis: a new approach to suppression and survival of hydatid cyst. Parasitol Res 110, 1979–1984 (2012). https://doi.org/10.1007/s00436-011-2726-4

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  • DOI: https://doi.org/10.1007/s00436-011-2726-4

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