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Supplementary studies on Henneguya doneci Schulman, 1962 (Myxozoa: Myxosporea) infecting the gill filaments of Carassius auratus gibelio (Bloch) in China: histologic, ultrastructural, and molecular data

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Abstract

Henneguya doneci Schulman, 1962 was collected from the gill filaments of Carassius auratus gibelio (Bloch) in Hubei Province, China. The plasmodia located on the surface of the gill arches deformed the neighboring gill filaments. The size of the plasmodia ranged from 0.6 to 4.5 mm in diameter in different months. The myxospores in the plasmodia measured 10.1 (9.2–11.5) μm long × 8.0 (7.5–8.5) μm wide × 7.5 (7–8) μm thick, with two equal capsules at 4.7 (4–5.5) μm long × 3.3 (2.5–4) μm wide, and two caudal processes 32.7 (24–38.5) μm long, respectively. Polar filaments were coiled 5–6 turns. Ultrastructural observation of the plasmodia showing the capsulogenesis of H. doneci is described briefly. The external tubule initially invaginated into the polar capsule. The rudimentary polar filaments were observed to undergo a series of considerable modification, finally developing into mature polar filaments. Molecular analysis demonstrated that although the myxosporean species were collected from different tissues of hosts in various geographic locations, they clustered with the Cyprinidae-infecting myxosporean species in the phylogenetic tree.

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Acknowledgements

The present study was financially supported by the earmarked fund for China Agriculture Research System (Grant no. CARS-46-08) and the National Program on Key Basic Research Project (Grant no. 2009CB118705). The authors thank Dr. Guo Xiang Liu for his help in photographing the fresh spores and Mr. Ao for his help in collecting the fish.

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Authors and Affiliations

  1. The Key Laboratory of Aquatic Biodiversity and Conservation, and the Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, Hubei Province, People’s Republic of China

    Ling Tong Ye, Wen Xiang Li, Shan Gong Wu & Gui Tang Wang

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Ling Tong Ye & Gui Tang Wang

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  1. Ling Tong Ye
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  2. Wen Xiang Li
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Correspondence to Gui Tang Wang.

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Ye, L.T., Li, W.X., Wu, S.G. et al. Supplementary studies on Henneguya doneci Schulman, 1962 (Myxozoa: Myxosporea) infecting the gill filaments of Carassius auratus gibelio (Bloch) in China: histologic, ultrastructural, and molecular data. Parasitol Res 110, 1509–1516 (2012). https://doi.org/10.1007/s00436-011-2655-2

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