Abstract
This study compared the prevalence of trypanosome infections estimated by PFR-loop-mediated isothermal amplification (LAMP) with conventional polymerase chain reaction (PCR) tests. One hundred forty eight cattle blood samples were collected from Robanda village, Mara region, Tanzania in April 2008. In conventional PCR, four sets of primers, specific for the detection of Trypanosoma sp., Trypanosoma brucei rhodesiense, Trypanosoma vivax, and Trypanozoon, as well as a modified LAMP were used. Conventional PCR detected no infection or up to 8, 1, and 3 infections with Trypanosoma congolense savannah, Trypanozoon, and T. vivax, respectively, whereas LAMP detected additional 44 Trypanozoon positive cases. Our results clearly indicate that the prevalence of Trypanozoon spp. in cattle in Robanda village estimated by PFR-LAMP (30.4%) was significantly higher than the estimates by PCR assays (0.6–2%). As such, future studies should target epidemiological surveys of Trypanozoon and T. brucei rhodesiense infections in possible reservoir animals by LAMP to further elucidate the actual prevalence of these parasites.
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Acknowledgments
The authors acknowledge the logistic support provided by the Directors for TTRI-Tanga and Yuka Suzuki, HUCZCZ-Lusaka, Zambia during this study. We also thank the Tanzania National Park Authority (TANAPA) and particularly the staff of Serengeti National Park Veterinary Laboratory for providing space and assistance. Our sincere gratitude also goes to Mr. Hamisi Nyingilili and Mr. Lupakisyo Kapange for their help of sampling. The authors thank all the farmers kindly allowing our sampling from their cows. This study was supported by a Grant-in-Aid for scientific Research from JSPS to SK, CS, and NI, and partially supported by GCOE program.
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Laohasinnarong, D., Thekisoe, O.M.M., Malele, I. et al. Prevalence of Trypanosoma sp. in cattle from Tanzania estimated by conventional PCR and loop-mediated isothermal amplification (LAMP). Parasitol Res 109, 1735–1739 (2011). https://doi.org/10.1007/s00436-011-2513-2
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DOI: https://doi.org/10.1007/s00436-011-2513-2