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Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein

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Abstract

Fasciola gigantica, causative agent of tropical fasciolosis, inflicts substantial economic losses on the livestock industry, affecting severely buffalo productivity in the tropical countries. Very few vaccination trials with different target antigens against F. gigantica infection have been conducted in this host. Present study describes a vaccination trial in buffaloes with F. gigantica recombinant glutathione S-transferase and fatty acid binding protein. The two recombinant proteins were expressed in Escherichia coli and evaluated for their immunoprophylactic potential in buffalo calves, using montanide 70 M-VG, a mineral oil-based adjuvant, for delivering the antigens. Buffalo calves were distributed in three groups, with group I, II and III calves immunized with recombinant glutathione S-transferase, fatty acid binding protein and a cocktail of these two antigens, respectively. Immunization of the calves evoked a mixed IgG1 and IgG2 antibody response. Present vaccination trial in these animals achieved a maximum protection level of 35%, when the two antigens were used in combination. Eosinophils were measured in both immunized and non-immunized challenge control animals, which showed a steady increase in their count in response to immunization with both the antigens and infection with F. gigantica, respectively.

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References

  • Agricultural Research Data Book (2002) Indian agricultural statistics. Research Institute, New Delhi, p 28

    Google Scholar 

  • Aiello SE, Mays A (1998) The Merck veterinary manual, 8th edn. Merck & Co Inc, New Jersey, pp 1196–1198

    Google Scholar 

  • Brophy PM, Pritchard DI (1994) Parasitic helminth glutathione S-transferases: an update on their potential as targets for immuno and chemotherapy. Exp Parasitol 79:89–96

    Article  PubMed  CAS  Google Scholar 

  • Butterworth AE (1984) Cell-mediated damage to helminthes. Adv Parasitol 23:143–235

    Article  PubMed  CAS  Google Scholar 

  • Chunchob S, Grams R, Viyanant V, Smooker PM, Vichasri-Grams S (2010) Comparative analysis of two fatty acid binding proteins from Fasciola gigantica. Parasitology 137:1805–1817

    Article  PubMed  CAS  Google Scholar 

  • Edith R, Godara R, Sharma RL, Thilagar MB (2010) Serum enzyme and hematological profile of Fasciola gigantica immunized and experimentally infected riverine buffaloes. Parasitol Res 106:947–956

    Article  PubMed  CAS  Google Scholar 

  • Estuningsih ES, Smooker PM, Wiedosari E, Widjajanti S, Vaiano A, Partotomo S, Spithill TW (1997) Evaluation of antigens of Fasciola gigantica as vaccine against tropical fasciolosis. Int J Parasitol 11:1419–1428

    Article  Google Scholar 

  • FAO (1994) Diseases of domestic animals caused by flukes. Food and Agriculture Organization of the United Nations, Rome, p 49

    Google Scholar 

  • Hansen DS, Clery DG, Estuningsih SE, Widjajanti S, Partoutomo S, Spithill TW (1999) Immune responses in Indonesian thin tail and Merino sheep during a primary infection with Fasciola gigantica: lack of a specific IgG2 antibody response is associated with increased resistance to infection in Indonesian sheep. Int J Parasitol 29:1027–1035

    Article  PubMed  CAS  Google Scholar 

  • Hillyer GV (1985) Induction of immunity in mice to Fasciola hepatica with a Fasciola/Schistosoma cross-reactive defined antigen. Am J Trop Med Hyg 34:1127–1131

    PubMed  CAS  Google Scholar 

  • Hillyer GV, Haroun EM, Hermandez A, De Galanes MS (1987) Acquired resistance to Fasciola hepatica in cattle using a purified adult worm antigen. Am J Trop Med Hyg 37:363–369

    PubMed  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:630–684

    Article  Google Scholar 

  • López-Abán J, Nogal-Ruiz JJ, Vicente B, Morrondo P, Diez-Baños P, Hillyer GV, Martínez-Fernández AR, Feliciano AS, Muro A (2008) The addition of a new immunomodulator with the adjuvant adaptation ADAD system using fatty acid binding proteins increases the protection against Fasciola hepatica. Vet Parasitol 153:176–181

    Article  PubMed  Google Scholar 

  • McTigue MA, Williams DR, Tainer JA (1995) Crystal structures of a schistosomal drug and vaccine target: glutathione S-transferase from Schistosoma japonicum and its complex with the leading antischistosomal drug praziquantel. J Mol Biol 246:21–27

    Article  PubMed  CAS  Google Scholar 

  • Mehra UR, Dass RS, Verma AK, Sharma RL, Yadav SC (1999) Effect of Fasciola gigantica infection on growth and nutrient utilization in buffalo calves. Vet Rec 145:699–702

    PubMed  CAS  Google Scholar 

  • Mendes RE, Pérez-Ecija RA, Zafra R, Buffoni L, Martínez-Moreno A, Dalton JP, Mulcahy G, Pérez J (2010) Evaluation of hepatic changes and local and systemic immune responses in goats immunized with recombinant peroxiredoxin and challenged with Fasciola hepatica. Vaccine 28:2832–2840

    Article  PubMed  CAS  Google Scholar 

  • Meyer F, Meyer H, Bueding E (1970) Lipid metabolism in the parasitic and free living flatworms. Schistosoma mansoni and Dugesia dorotocephala. Biochim Biophys Acta 210:257–266

    PubMed  CAS  Google Scholar 

  • Mitchell GF (1989) Glutathione S-transferase: potential component of antischistosomal vaccines. Parasitol Today 5:34–57

    Article  PubMed  CAS  Google Scholar 

  • Morrison CA, Colin T, Sexton JL, Bowen F, Wicker J, Friedel T, Spithill TW (1996) Protection of cattle against Fasciola hepatica infection by vaccination with glutathione S-transferase. Vaccine 14:1603–1612

    Article  PubMed  CAS  Google Scholar 

  • Mulcahy G, O'Connor G, Clery D, Hogan SF, Dowd AJ, Andrews SJ, Dalton JP (1999) Immune responses to cattle to experimental anti-Fasciola hepatica vaccines. Res Vet Sci 67:27–33

    Article  PubMed  CAS  Google Scholar 

  • Muro A, Ramajo V, Lopez J, Simon F, Hillyer GV (1997) Fasciola hepatica: vaccination of rabbits with recombinant and native antigens related to fatty acid binding proteins. Vet Parasitol 69:219–229

    Article  PubMed  CAS  Google Scholar 

  • Muro A, Casanueva P, López-Abán J, Ramajo V, Martínez-Fernández AR, Hillyer GV (2007) Identification of Fasciola hepatica recombinant 15-kDa fatty acid-binding protein T-cell epitopes that protect against experimental fascioliasis in rabbits and mice. J Parasitol 93:817–823

    Article  PubMed  CAS  Google Scholar 

  • Nambi PA, Yadav SC, Raina OK, Sriveny D, Saini M (2005) Vaccination of buffaloes with Fasciola gigantica recombinant fatty acid binding protein. Parasitol Res 97:129–135

    Article  PubMed  Google Scholar 

  • Ockner RK (1990) Historic overviews of the studies on fatty acid binding proteins. Mol Cell Biochem 98:3–9

    Article  PubMed  CAS  Google Scholar 

  • Piedrafita D, Estuningsih E, Pleasance J, Prowse R, Raadsma HW, Meeusen Els NT, Spithill TW (2007) Peritoneal lavage cells of Indonesian thin-tail sheep mediate antibody-dependent superoxide radical cytotoxicity in vitro against newly excysted juvenile Fasciola gigantica but not juvenile Fasciola hepatica. Infect Immun 75:1954–1963

    Article  PubMed  CAS  Google Scholar 

  • Preyavichyapugdee N, Sahaphong S, Riengrojpitak S, Grams R, Viyanant V, Sobhon P (2008) Fasciola gigantica and Schistosoma mansoni: vaccine potential of recombinant glutathione S-transferase (rFgGST26) against infections in mice. Exp Parasitol 119:229–237

    Article  PubMed  CAS  Google Scholar 

  • Radostits OM, Blood DC, Gay CC (1994) Veterinary medicine-a text book of the diseases of cattle, sheep, pigs, goats and horses, 8th edn. Bailliere Tindall, London, pp 1230–1236

    Google Scholar 

  • Ramajo V, Oleaga A, Casanueva P, Hillyer GV, Muro A (2001) Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins. Vet Parasitol 97:35–46

    Article  PubMed  CAS  Google Scholar 

  • Rossjohn J, Feil SC, Wilee MCL, Sexton JL, Spithill TW, Parker MW (1997) Crystallization structural determination and analysis of a novel parasite vaccine candidate Fasciola hepatica GST. J Mol Biol 273:857–872

    Article  PubMed  CAS  Google Scholar 

  • Sanyal PK (2001) Control of tropical fasciolosis in cattle and buffaloes in India at the backdrop of its integrated management. J Vet Parasitol 15:13–16

    Google Scholar 

  • Sexton JL, Milner AR, Panaccio M, Waddington J, Wijffels G, Chandler D, Thompson C, Wilson L, Spithill TW, Mitchell GF, Campbell NJ (1990) Glutathione S-transferase. novel vaccine against Fasciola hepatica infection in sheep. J Immunol 145:3905–3910

    PubMed  CAS  Google Scholar 

  • Sexton JL, Wilce M, Colin T, Wijffels GL, Salvatore L, Feil S, Parker MW, Spithill TW, Morrison CA (1994) Vaccination of sheep against Fasciola hepatica with glutathione S-transferase: identification and mapping of antibody epitopes on a three dimensional model of the antigen. J Immunol 152:1861–1872

    PubMed  CAS  Google Scholar 

  • Tendler M, Brito CA, Vilar MM, Serra-Freire N, Diogo CM, Almeida MS, Delbem AC, De Silva JF, Sanivo W, Garratt RC, Simpson AJ (1996) A Schistosoma mansoni fatty acid binding protein Sm14 is the potential basis of dual purpose anti-helminth vaccine. Proc Natl Acad Sci U S A 93:269–273

    Article  PubMed  CAS  Google Scholar 

  • Wijffels GL, Salvatore L, Dosen M, Waddington J, Wilson L, Thompson C, Campbell N, Sexton JL, Wicker J, Bowen F, Spithill TW (1994) Vaccination of sheep with purified cysteine proteinases of Fasciola hepatica decreases worm fecundity. Exp Parasitol 78:132–148

    Article  PubMed  CAS  Google Scholar 

  • Yadav SC, Sharma RL, Kalicharan A, Mehra UR, Das RS, Verma AK (1999) Primary experimental infection of riverine buffaloes with Fasciola gigantica. Vet Parasitol 82:285–296

    Article  PubMed  CAS  Google Scholar 

  • Zhang WY, Moreau E, Yang BZ, Li ZQ, Hope JC, Howard CJ, Huang WY, Chauvin A (2006) Humoral and cellular immune responses to Fasciola gigantica experimental infection in buffaloes. Res Vet Sci 80:299–307

    Article  PubMed  CAS  Google Scholar 

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Acknowledgements

The authors are thankful to the Department of Biotechnology, Government of India, New Delhi for providing grants to this research project. We are also thankful to the Director, Indian Veterinary Research Institute, Izatnagar for providing necessary facilities for completion of this reserach work.

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Authors and Affiliations

  1. Division of Parasitology, Indian Veterinary Research Institute, Izatnagar, 243122, India

    Niranjan Kumar, Varghese Anju, Nagar Gaurav, Dinesh Chandra, S. Samanta, S. C. Gupta, J. Adeppa & O. K. Raina

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  1. Niranjan Kumar
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  2. Varghese Anju
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  3. Nagar Gaurav
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  4. Dinesh Chandra
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  5. S. Samanta
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  6. S. C. Gupta
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  7. J. Adeppa
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  8. O. K. Raina
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Correspondence to O. K. Raina.

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Kumar, N., Anju, V., Gaurav, N. et al. Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein. Parasitol Res 110, 419–426 (2012). https://doi.org/10.1007/s00436-011-2507-0

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  • DOI: https://doi.org/10.1007/s00436-011-2507-0

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