Abstract
Bed bug, Cimex lectularius L., populations were investigated for resistance against permethrin and chlorpyrifos in a topical application bioassay, after an initial establishment of a discriminating dose with a susceptible population. For both insecticides, ca. two times the lethal dose LD99 was selected: 2,560 ng of permethrin and 200 ng of chlorpyrifos per bed bug, respectively. Bed bugs were collected from infested homes in Denmark at ten locations and bred in the laboratory. The frequency of permethrin-resistant individuals was high in Danish bed bug populations as susceptible individuals were only found in three of ten populations. In contrast, the frequency of chlorpyrifos-resistant individuals was low in Danish bed bug populations, but resistant individuals were found in five of ten populations. To test the significance of the observed resistance, we performed tarsal contact test with commercially available insecticides. The test indicated that both a permethrin and a deltamethrin product had very low efficacy against the field-collected bed bug populations. Despite the reduced sensitivity to synthetic pyrethroids, all populations tested in the tarsal test on the commercial product with micro-encapsulated chlorpyrifos resulted in close to 100% mortality.
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Acknowledgments
We thank Harold Harlan for providing us with bed bugs from his laboratory colony and Nicolai Hansen and Kristian Hansen for technical assistance. This study was supported by the Danish Environmental Protection Agency.
Ethical standards
All experiments comply with the current laws of Denmark.
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The authors declare they have no conflicts of interest
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Kilpinen, O., Kristensen, M. & Vagn Jensen, KM. Resistance differences between chlorpyrifos and synthetic pyrethroids in Cimex lectularius population from Denmark. Parasitol Res 109, 1461–1464 (2011). https://doi.org/10.1007/s00436-011-2423-3
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DOI: https://doi.org/10.1007/s00436-011-2423-3