Abstract
Abdominal angiostrongyliasis (AA) is caused by the nematode Angiostrongylus costaricensis. Parasite-associated thrombosis of mesenteric vessels may lead to intestinal infarction, which might be prevented with anti-thrombotic agents. This study assessed the effect of enoxaparin on survival and pathological findings in Swiss mice with AA. In this experiment, 24 mice were infected with A. costaricensis (10 L3 per animal) followed by treatment with subcutaneous enoxaparin (40 mg/kg/day) or water (sham), starting from 15 days post-infection (dpi) and continued until animal death. Animals were monitored until death or sacrifice at the 50th dpi. Ten mice (42%) were dead after 36 ± 8 dpi. Of these, five (50%) were treated with enoxaparin. Animals treated with enoxaparin and sham did not differ in terms of weight loss (median, 1.3 vs. 4.2 g; P = 0.303) and macroscopical findings. Microscopically, no difference was found in regard to vascular granuloma (median grade, 2 vs. 3; P = 0.293) and presence of either vasculitis (75% vs. 100%; P = 0.217), mesenteric thrombosis (33% vs. 50%; P = 0.680), or bowel necrosis (25% vs. 50%; P = 0.400). Mice dead before the 50th dpi showed more pneumonia (90% vs. 21%; P = 0.002), bowel infarction (40% vs. 0%; P = 0.02), and purulent peritonitis (60% vs. 7%; P = 0.008) compared to survivors. Prophylactic enoxaparin in mice did not prevent tissue damage and mortality related with AA. The lower prevalence of mesenteric thrombosis and bowel infarction regardless of treatment were notorious. Frequent septic complications suggest the need of studies addressing the effect of antibiotics in AA.
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References
Agostini AA, Marcolan AM, Lisot JM, Lisot JU (1984) Abdominal angiostrongyliasis. Anatomo-pathological study of 4 cases observed in Rio Grande do Sul, Brazil. Mem Inst Oswaldo Cruz 79:443–445
Bohrer MM, Dallegrave E, Agostini A, Graeff-Teixeira C (2007) Phenantroline, lovastatin, and mebendazole do not inhibit oviposition in the murine experimental infection with Angiostrongylus costaricensis. Parasitol Res 100:379–382
de Azevedo GV, Rodriguez R, Porto SM, Graeff-Teixeira C, Fornari F (2010) Elimination of Angiostrongylus costaricensis larvae in feces from experimentally infected Swiss mice: circadian rhythm and correlation with survival. Parasitol Res. doi:10.1007/s00436-010-2094-5
Fante CA, Dieterish S, Rodriguez R (2008) Betamethasone and aqueous extract of Arctium lappa for treating angiostrongyliasis. Rev Soc Bras Med Trop 41:654–657
Graeff-Teixeira C, Camillo-Coura L, Lenzi HL (1991) Histopathological criteria for the diagnosis of abdominal angiostrongyliasis. Parasitol Res 77:606–611
Graeff-Teixeira C, Goulart AH, de Brum CO, Laitano AC, Sievers-Tostes C, Zanini GM, Bered PL, Morassutti A, Geiger S, Brahms-Sandi E, Oliveira FT, Maurer RL, Aguiar LF, Garrido CT, da Silva AC, Rodriguez R, Schulz-Key H (2005) Longitudinal clinical and serological survey of abdominal angiostrongyliasis in Guapore, southern Brazil, from 1995 to 1999. Rev Soc Bras Med Trop 38:310–315
Mentz MB, Graeff-Teixeira C (2003) Drug trials for treatment of human angiostrongyliasis. Rev Inst Med Trop São Paulo 45:179–184
Morera P (1970) Studies of the definitive host of Angiostrongylus costaricensis. (Morera and Cespedes, 1971). Bol Chil Parasitol 25:133–134
Morera P (1973) Life history and redescription of Angiostrongylus costaricensis Morera and Cespedes, 1971. Am J Trop Med Hyg 22:613–621
Morera P (1985) Abdominal angiostrongyliasis: a problem of public health. Parasitol Today 1:173–175
Morera P, Ash LR (1970) Studies on the intermediate host of Angiostrongylus costaricensis (Morera and Cespedes, 1971). Bol Chil Parasitol 25:135
Morera P, Bontempo I (1985) Acción de algunos antihelmínticos sobre Angiostrongylus costaricensis. Rev Méd Hosp Nac Niños (Costa Rica) 20:165–174
Morera P, Cespedes R (1970) Angiostrongylus costaricensis n. sp. (Nematoda: Metastrongyloidea), a new lungworm occurring in man in Costa Rica. Rev Biol Trop 18:173–185
Morera P, Céspedes R (1971) Angiostrongilosis Abdominal. Acta Méd Cost 14:159–173
Morera P, Lazo R, Urquizo J, Llaguno M (1983) First record of Angiostrongylus costaricensis Morera and Cespedes, 1971 in Ecuador. Am J Trop Med Hyg 32:1460–1461
Rodriguez R (1997) Anatomia patológica da angiostrongilose abdominal. Rio de Janeiro. Dissertação de Mestrado—Universidade Federal Fluminense
Rodriguez R, Dequi RM, Peruzzo L, Mesquita PM, Garcia E, Fornari F (2008) Abdominal angiostrongyliasis: report of two cases with different clinical presentations. Rev Inst Med Trop São Paulo 50:339–341
Ubelaker JE, Hall NM (1979) First report of Angiostrongylus costaricensis Morera and Cespedes 1971 in the United States. J Parasitol 65:307
Vazquez JJ, Boils PL, Sola JJ, Carbonell F, de Juan BM, Giner V, Berenguer-Lapuerta J (1993) Angiostrongyliasis in a European patient: a rare cause of gangrenous ischemic enterocolitis. Gastroenterology 105:1544–1549
Waisberg J, Corsi CE, Rebelo MV, Vieira VT, Bromberg SH, dos Santos PA, Monteiro R (1999) Jejunal perforation caused by abdominal angiostrongyliasis. Rev Inst Med Trop São Paulo 41:325–328
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Rodriguez, R., Porto, S.M., dos Santos Ferrari, R. et al. Outcomes in mice with abdominal angiostrongyliasis treated with enoxaparin. Parasitol Res 109, 787–792 (2011). https://doi.org/10.1007/s00436-011-2324-5
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DOI: https://doi.org/10.1007/s00436-011-2324-5