Abstract
Diphyllobothrium latum is a widespread intestinal parasite, which has a great clinical relevance, but there are no sequences of its nuclear genome. In this paper, a repetitive element in the D. latum genome is firstly described. The adult D. latum was obtained in the result of expulsion from intestinum of a patient suffering from diphyllobothriasis. Genomic DNA was isolated from several proglottids of this individual. PstI restriction products of D. latum genomic DNA were sequenced. Polymerase chain reaction (PCR) amplification of these products using genomic DNA and selected primers was carried out. Thereby a cluster of a repetitive element, called DL1, was discovered. For precise identification of a beginning and an end of the repeat, a product of PCR amplification of D. latum genomic DNA with one specific primer was sequenced. In discussion, several evidences that DL1 repeat is a member of the SINE family of retroposons were adduced.
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Notes
The nucleotide sequences data reported in this paper have been submitted to GenBank with the accession numbers GQ398237 and GU350458.
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Research described in this article was supported by the Program of Presidium of the Russian Academy of Sciences “Molecular and Cellular Biology”.
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Usmanova, N.M., Kazakov, V.I. The DL1 repeats in the genome of Diphyllobothrium latum . Parasitol Res 107, 449–452 (2010). https://doi.org/10.1007/s00436-010-1889-8
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DOI: https://doi.org/10.1007/s00436-010-1889-8