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Susceptibility of Aedes aegypti (Diptera: Culicidae) to Acanthamoeba polyphaga (Sarcomastigophora: Acanthamoebidae)

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Abstract

To date there is no report on mosquitoes infected with free-living amoebae. For this reason, the aim of this study was to verify if Aedes aegypti could be susceptible to Acanthamoeba polyphaga under laboratory conditions, so trophozoites were offered as a unique food resource for larvae of first instar. The results show that those amoebae are able to infect and colonize the mosquito gut and could be re-isolated of all stages of the mosquito (larvae, pupae, and adults).

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References

  • Albicócco AP, Vezzani D (2009) Further study on Ascogregarina culicis in temperate Argentina: prevalence and intensity in Aedes aegypti larvae and pupae. J Invertebr Pathol 101(3):210–214

    Article  PubMed  Google Scholar 

  • Azambuja P, Garcia ES, Ratcliffe NA (2005) Gut microbiota and parasite transmission by insect vectors. Trends Parasitol 21(12):568–572

    Article  PubMed  Google Scholar 

  • Beier JC, Craig GB Jr (1985) Gregarine parasites of mosquitoes. In: Laird M, Miles JW (eds) Integrated mosquito control methodologies, vol 2. Academic Press, London, pp 167–184

    Google Scholar 

  • Carlesso AM, Artuso GL, Caumo K, Rott MB (2009) Potentially pathogenic Acanthamoeba isolated from a hospital in Brazil. Curr Microbiol 60(3):185–190

    Article  PubMed  Google Scholar 

  • Caumo K, Frasson AP, Pens C, Panatieri LF, Frason APG, Rott MB (2009) Potentially pathogenic Acanthamoeba in swimming pools: a survey in the southern Brazilian city of Porto Alegre. Ann Trop Med Parasitol 103:477–485

    Article  CAS  PubMed  Google Scholar 

  • Chen CD, Lee HL, Nazni WA, Seleena B, Lau KW, Daliza AR, Ella Syafinas S, Mohd Sofian A (2009) Field effectiveness of Bacillus thuringiensis israelensis (Bti) against Aedes (Stegomyia) aegypti (Linnaeus) in ornamental ceramic containers with common aquatic plants. Trop Biomed 26(1):100–105

    CAS  PubMed  Google Scholar 

  • Chesson J (1984) Effect of notonectids (Hemiptera: Notonectidae) on mosquitoes (Diptera: Culicidae): predation or selective oviposition? Environ Entomol 13:531–538

    Google Scholar 

  • Dos Passos RA, Tadei WP (2008) Parasitism of Ascogregarina taiwanensis and Ascogregarina culicis (Apicomplexa: Lecudinidae) in larvae of Aedes albopictus and Aedes aegypti (Diptera: Culicidae) from Manaus, Amazon region, Brazil. J Invertebr Pathol 97(3):230–236

    Article  PubMed  Google Scholar 

  • Fukuda T, Osborne RW, Barnard DR (1997) Parasites of the Asian tiger mosquito and other container-inhabiting mosquitoes (Diptera: Culicidae) in northern Florida. J Med Entomol 34:226–233

    CAS  PubMed  Google Scholar 

  • Garcia JJ, Fukuda T, Becnel JJ (1994) Seasonality, prevalence and pathogenicity of the gregarina Ascogregarina taiwanensis (Apicomplexa: Lecudinidae) in mosquitoes from Florida. J Am Mosq Control Assoc 10:413–418

    CAS  PubMed  Google Scholar 

  • Huang CG, Tsai KH, Wu WJ, Chen WJ (2006) Intestinal expression of H + V-ATPase in the mosquito Aedes albopictus is tightly associated with gregarine infection. J Eukaryot Microbiol 53(2):127–135

    Article  CAS  PubMed  Google Scholar 

  • Khan NA (2006) Acanthamoeba: biology and increasing importance in human health. FEMS Microbiology Review 30:564–595

    Article  Google Scholar 

  • Marciano-Cabral F, Puffenbarger R, Cabral GA (2000) The increasing importance of Acanthamoeba infections. J Eukaryot Microbiol 47:29–36

    Article  CAS  PubMed  Google Scholar 

  • Marten GG, Borjas G, Cush M, Fernández E, Reid JW (1994) Control of larval Aedes aegypti in peridomestic breeding containers. J Med Entomol 31:36–44

    CAS  PubMed  Google Scholar 

  • Martinez-Ibara JA, Guillén YG, Arredondo-Jimenes JI, Rodriguez-Lopes MH (2002) Indigenous fish species for the control of Aedes aegypti in water storage tanks in Southern México. Biocontrol 47:481–486

    Article  Google Scholar 

  • Moncayo AC, Lerdthusnee K, Leon R, Robich RM, Romoser WS (2005) Meconial peritrophic matrix structure, formation, and meconial degeneration in mosquito pupae/pharate adults: histological and ultrastructural aspects. J Med Entomol 42(6):939–944

    Article  PubMed  Google Scholar 

  • Page FC (1988) A new key to freshwater and soil gymnamoebae. Freshwater Biological Association, Cumbria, p 122

    Google Scholar 

  • Pens C, Costa M, Fadanelli C, Caumo K, Rott MB (2008) Acanthamoeba spp. and bacterial contamination in contact-lens storage cases and the relationship to user profiles. Parasitol Res 103:1241–1245

    Article  PubMed  Google Scholar 

  • Polson K, Rawlins S, Hylton H (2002) Relative sensitive of various Caribean strains of Aedes aegypti larvae to insecticides based on a simple bioassaay. Resis Pest Manag 11(2):13–16

    Google Scholar 

  • Rani A, Sharma A, Rajagopal R, Adak T, Bhatnagar RK (2009) Bacterial diversity analysis of larvae and adult midgut microflora using culture-dependent and culture-independent methods in lab-reared and field-collected Anopheles stephensi—an Asian malarial vector. BMC Microbiol 19(9):96

    Article  Google Scholar 

  • Reyes-Villanueva F, Becnel JJ, Butler JF (2003) Susceptibility of Aedes aegypti and Aedes albopictus larvae to Ascogregarina culicis and Ascogregarina taiwanensis (Apicomplexa: Lecudinidae) from Florida. J Invertebr Pathol 84:47–53

    Article  PubMed  Google Scholar 

  • Rodriguez MM, Bisset JÁ, Diaz C, Soca LA (2003) Resistência cruzada a piretroides en Aedes aegypti de Cuba inducido por la selecciûn con el insecticida organofosforado malation. Rev Cubana Med Trop 55(2):105–111

    PubMed  Google Scholar 

  • Roychoudhury S, Kobayashi M (2006) New findings on the developmental process of Ascogregarina taiwanensis and Ascogregarina culicis in Aedes albopictus and Ae. aegypti. J Am Mosq Control Assoc 22(1):29–36

    Article  PubMed  Google Scholar 

  • Roychoudhury S, Isawa H, Hoshino K, Sasaki T, Saito N, Sawabe K, Kobayashi M (2007) Comparison of the morphology of oocysts and the phylogenetic analysis of four Ascogregarina species (Eugregarinidae: Lecudinidae) as inferred from small subunit ribosomal DNA sequences. Parasitol Int 56(2):113–118

    Article  CAS  PubMed  Google Scholar 

  • Santamarina MA, Pez PR (1998) Efecto patogenico del nematodo Romanomermis culicivorax (Nematoda: Mermithidae) en larvas de Aedes (S) aegypti (Linnaeus, 1762) (Diptera: Culicidae) en condiciones de laboratorio em el estado de Oaxaca, México. Rev Cubana Med Trop 50:8–11

    Google Scholar 

  • Santamarina MA, Perez PR, Pacheco R, Martinez SH (2000) Susceptibilidad de las larvas de Aedes aegypti al parasitismo por Romanomermis culicivorax en condiciones de campo en Oaxaca, México. Rev Pan Salud Publica 8(5):299–304

    Google Scholar 

  • Schreiber ET, Hallmon CF, Eskridge KM, Marten GG (1996) Effects of Mesocyclops longisetus (Copepoda: Cyclopoidea) on mosquitoes that inhabit tires: influence of liter type, quality, and quantity. J Am Mosq Control Assoc 8:409–412

    Google Scholar 

  • Schuster FL, Visvesvara GS (2004) Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. J Parasitol 34:1001–1027

    Google Scholar 

  • Visvesvara GS, Moura H, Schuster FL (2007) Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea. FEMS Immunol Med Microbiol 50:1–26

    Article  CAS  PubMed  Google Scholar 

  • WHO (World Health Organization) (1997) Dengue haemorrhagic fever: diagnosis, treatment, prevention and control, 2nd edn. Geneva

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Authors and Affiliations

  1. Microbiologia Imunologia e Parasitologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Marilise Rott, Karin Caumo, Ismael Sauter, Janina Eckert, Luana da Rosa & Onilda da Silva

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  1. Marilise Rott
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  2. Karin Caumo
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  3. Ismael Sauter
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  4. Janina Eckert
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  5. Luana da Rosa
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  6. Onilda da Silva
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Correspondence to Marilise Rott.

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Rott, M., Caumo, K., Sauter, I. et al. Susceptibility of Aedes aegypti (Diptera: Culicidae) to Acanthamoeba polyphaga (Sarcomastigophora: Acanthamoebidae). Parasitol Res 107, 195–198 (2010). https://doi.org/10.1007/s00436-010-1843-9

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  • DOI: https://doi.org/10.1007/s00436-010-1843-9

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