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Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes

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Abstract

The ability to reliably detect anthelmintic resistance is a crucial part of resistance management. If data between countries are to be compared, the same test should give the same results in each laboratory. As the egg hatch test for benzimidazole resistance is used for both research and surveys, the ability of different laboratories to obtain similar results was studied through testing of known isolates of cyathostomins, Haemonchus contortus, Ostertagia ostertagi, and Cooperia oncophora in programs supported by the EU (Cost B16 and FP6-PARASOL). Initial results showed difficulties in obtaining reproducible and similar data within and between laboratories. A series of ring tests, i.e., simultaneous and coordinated rounds of testing of nematode isolates in different laboratories was subsequently performed. By adopting identical protocols, especially the use of deionized water and making dilutions of thiabendazole in dimethyl sulfoxide in the final ring test, laboratories correctly identified both susceptible and resistant isolates. The protocols for the test and preparation of solutions of thiabendazole are described.

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Acknowledgments

The financial support through the EU-Cost action B16 and the FP6 PARASOL (FOOD-CT-2005-022851) project is thankfully acknowledged. We are most thankful to all colleagues from the laboratories listed in Table 1 involved in performing the ring tests.

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Authors and Affiliations

  1. Institute for Parasitology, University of Veterinary Medicine, Hannover, Germany

    Georg von Samson-Himmelstjerna, Janina Demeler, Christian Epe & Ulla Küttler

  2. Department of Clinical Veterinary Science, University of Bristol, Bristol, BS40 5DU, UK

    Gerald C. Coles

  3. Moredun Research Institute, Midlothian, Scotland, UK

    Frank Jackson, Alison Donnan & John Small

  4. Institute of Parasitology, Justus Liebig University, Gieβen, Germany

    Christian Bauer

  5. Central Veterinary Institute of Wageningen UR, Lelystad, The Netherlands

    Fred Borgsteede

  6. Department of Parasitology, Uludag University, Bursa, Turkey

    Veli Y. Cirak

  7. Institute of Tropical Medicine, Antwerp, Belgium

    Pierre Dorny

  8. Novartis Centre de Recherche Santé Animale, St. Aubin, Switzerland

    Christian Epe & Ronald Kaminsky

  9. BayerHealthcare Animal Health, Leverkusen, Germany

    Achim Harder

  10. Department of Parasitology (SWEPAR), Swedish University of Agricultural Sciences, Uppsala, Sweden

    Johan Höglund

  11. INRA, UR-1282, IASP, 37380, Nouzilly, France

    Dominique Kerboeuf

  12. Laboratory of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, Thessaloniki, Greece

    Elias Papadopoulos

  13. Faculty of Veterinary Medicine, University of Ljubljana, Ljubljana, Slovenia

    Janez Posedi

  14. Parasitological Institute, Slovak Academy of Science, Kosice, Slovak Republic

    Marián Várady

  15. Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium

    Jozef Vercruysse

  16. Pfizer Animal Health, Sandwich, UK

    Nicole Wirtherle

Authors
  1. Georg von Samson-Himmelstjerna
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  2. Gerald C. Coles
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  3. Frank Jackson
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  4. Christian Bauer
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  5. Fred Borgsteede
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  6. Veli Y. Cirak
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  7. Janina Demeler
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  8. Alison Donnan
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  9. Pierre Dorny
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  10. Christian Epe
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  11. Achim Harder
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  12. Johan Höglund
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  13. Ronald Kaminsky
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  14. Dominique Kerboeuf
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  15. Ulla Küttler
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  16. Elias Papadopoulos
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  17. Janez Posedi
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  18. John Small
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  19. Marián Várady
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  20. Jozef Vercruysse
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  21. Nicole Wirtherle
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Corresponding author

Correspondence to Georg von Samson-Himmelstjerna.

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von Samson-Himmelstjerna, G., Coles, G.C., Jackson, F. et al. Standardization of the egg hatch test for the detection of benzimidazole resistance in parasitic nematodes. Parasitol Res 105, 825–834 (2009). https://doi.org/10.1007/s00436-009-1466-1

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  • DOI: https://doi.org/10.1007/s00436-009-1466-1

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