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Detection of tick blood parasites in Egypt using PCR assay I—Babesia bovis and Babesia bigemina

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Abstract

Babesia bovis and Babesia bigemina are distributed all over the world; the etiologic agents of the animal babesiosis are considered the most important tick-borne disease. The present research work was the first attempt to determine the prevalence of B. bovis and B. bigemina infection in ticks, in Egypt, by using polymerase chain reaction (PCR). Questing 5,243 hard and soft ticks were collected from different localities throughout the Giza Governorate. Furthermore, DNA from 500 different individual tick species was extracted and PCR was performed. Primers verified from the sequence of Mexico strain of both species were used. Two fragments of 275 and 175 bp of B. bovis and B. bigemina, respectively, were generated. Fragments of the pathogens were recovered with PCR and sequenced. The prevalence of B. bovis and B. bigemina in Boophilus annulatus ticks were 55% and 66%, respectively. Also, presence of 12% dual infection with B. bovis and B. bigemina was observed. Sequence analysis of PCR product of these pathogens shares a high degree of similarity in sequence compared to similar species found in GenBank.

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References

  • Abd-El Gawad M (1993) Some studies on Babesia species in cattle in Beni-Suef governorate. Ph.D. Thesis, Fac. Vet. Med., Cairo Univ., Giza, Egypt

  • Abo-Elkheir EA (1989) Epidemiological aspect on the distribution of haemotrophic parasites among cattle in Upper Egypt confirmed by the identification of encountered organisms. M.Sc. Thesis, Fac. Vet. Med., Cairo Univ., Giza, Egypt

  • Ahmed AB (1980) Some biological studies on blood parasites of farm animals. Ph.D. Thesis, Fac. Vet. Med., Zagazig Univ., Zagazig, Egypt

  • Altschul SF, Madden TL, Schäfferl AA, Zhang J, Zheng Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  CAS  PubMed  Google Scholar 

  • Calder JA (1994) Improved diagnosis of bovine babesiosis. Ph.D. Thesis, Fac. Vet. Med. Florida Univ., Gainesville, FL, USA

  • Chafick ML (1987) Serological tests for the field diagnosis of Babesia infection in claves. M.Sc. Thesis, Fac. Vet. Med., Cairo Univ., Cairo, Egypt

  • Drummond RO, George JE, Kunz SE (ed) (1988) Control of arthropod pests of livestock. Rev. Tech., CRC Press, Boca Raton, pp 1–19 and 145–158

  • El-Allawy TA (1973) Some studies on bovine piroplasmosis in Assiut. M.Sc. Thesis, Fac. Vet. Med., Assiut Univ., Assiut, Egypt

  • El-Ghaysh AA (1993) Studies on Babesia sp. infecting cattle and water buffaloes in Egypt. Ph.D. Thesis, Fac. Vet. Med., Cairo Univ. Giza, Egypt

  • Fadly RS (1996) Serological studies on Babesia in Behera Province M.Sc. Thesis, Fac. Vet. Med. Alexandria Univ. Alexandria, Egypt

  • Fahrimal Y, Goff WL, Jasmer DP (1992) Detection of Babesia bovis carrier cattle by using polymerase chain reaction amplification of parasite DNA. J Clin Microbiol 30:2091–2096

    Google Scholar 

  • Figueroa JV, Chieves LP, Johnson GS, Buening GM (1993) Multiplex polymerase chain reaction based assay for detection of Babesia bigemina, Babesia bovis and Anaplasma marginale DNA in bovine blood. Vet Parasitol 50:69–81

    Article  CAS  PubMed  Google Scholar 

  • Gattas MW (1983) Some studies on cattle Babesiosis in Suez Canal zone. M.Sc. Thesis, Fac. Vet. Med., Cairo Univ., Giza, Egypt

  • Gattas MW (1990) Further studies on the biological aspect of Babesia species in Suez Canal zone with special references to their control. Ph.D. Thesis, Cairo Univ., Giza, Egypt

  • Higgins JA, Azad AF (1995) Use of polymerase chain reaction to detect bacteria in arthropods: a review. J Med Entomol 32:213–222

    CAS  PubMed  Google Scholar 

  • Hoogstraal H (1981) Changing patterns of tick borne diseases in modern society. Ann Rev Entomol 26:75–99

    Article  CAS  Google Scholar 

  • Mahoney DF (1969) Bovine babesiosis: a study of factors concerned in transmission. Ann Trop Parasitol 63:1–14

    CAS  Google Scholar 

  • Martins TM, Pedro OC, Caldeira RA, Do Rosário VE, Neves L, Domingos A (2008) Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method. Vet Parasitol 153:225–230

    Article  CAS  PubMed  Google Scholar 

  • Mohan RN (1968) Diseases and parasites of buffaloes. Vet Bull 38:647–659

    Google Scholar 

  • Morzaria S, Katende J, Kairo A, Nene V, Musoke A (1992) New methods for the diagnosis of Babesia bigemina infection. Mem Inst Oswaldo Cruz Rio J 87(Suppl 3):201–205

    Google Scholar 

  • Nagati HE (1947) Some new and rare records of piroplasmosis with a list of the species of Babesia and Theileria so far recorded from Egypt. Vet Rec 59:145–147

    Google Scholar 

  • Oliveira-Sequeira TCG, Oliveira MCS, Araujo JP, Amarante AFT (2005) PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle. Int J Parasitol 35:105–111

    Article  CAS  PubMed  Google Scholar 

  • Reddy GR, Charkarbari D, Yowell CA, Dame JB (1991) Sequence microheterogeneity of the three small subunit ribosomal RNA genes of B. bigemina expression in erythrocyte culture. Nucleic Acids Res 19:3641–3645

    Article  CAS  PubMed  Google Scholar 

  • Roberts LS, Janovy J (2005) Foundations of parasitology, 7th edn. McGraw Hill, New York, pp 644–646

    Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 23:488–491

    Google Scholar 

  • Sakla AA (1975) Studies on ticks in Assiut Governorate, with special reference to their role in transmission of parasitic diseases. M.Sc. Thesis, Fac. Vet. Med., Assiut Univ., Assiut, Egypt

  • Salem GH (1998) Using of ribosomal DNA based PCR method for sensitive detection of carrier and mild babesial infection among cattle in Egypt. Assiut Vet Med J 39:92–108

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

    Google Scholar 

  • Todorovic RA (1975) Serological diagnosis of babesiosis: a review. Trop Anim Health Prod 7:1–14

    Article  Google Scholar 

  • Wall R, Shearer D (ed) (1997) Veterinary entomology. Chapman and Hall, London, pp 97–140 and 132–133

  • Waters AP, McCutchan TF (1990) Ribosomal RNA, nature’s own polymerase-amplified target for diagnosis. Parasit Today 6:56–59

    Article  CAS  Google Scholar 

  • Weiland G, Reiter I (1988) Methods for measurement of the serological response to Babesia. In: Ristic M (ed) Babesiosis of domestic animals and man. CRC Press, Boca Raton, pp 143–162

    Google Scholar 

  • Wittenbrink MM, Thiele D, Krauss H (1994) Comparison of dark-field microscopy, culture, and polymerase chain reaction (PCR) for detection of Borrelia burgdorferi in field-collected Ixodes ricinus ticks. Zentralbl Bakteriol 281:183–191

    CAS  PubMed  Google Scholar 

  • Zhang M, Gothe R, Rinder H (1995) Detection of spotted fever group rickettsiae in ticks and rodents by PCR technique in People’s Republic of China. Acta Virol 39:263–267

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Entomology Department, Faculty of Science, Cairo University, Giza, Egypt

    F. K. Adham, E. M. Abd-El-Samie, R. M. Gabre & H. El. Hussein

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  1. F. K. Adham
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  2. E. M. Abd-El-Samie
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  3. R. M. Gabre
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  4. H. El. Hussein
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Correspondence to H. El. Hussein.

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Adham, F.K., Abd-El-Samie, E.M., Gabre, R.M. et al. Detection of tick blood parasites in Egypt using PCR assay I—Babesia bovis and Babesia bigemina . Parasitol Res 105, 721–730 (2009). https://doi.org/10.1007/s00436-009-1443-8

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