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Schistosoma japonicum: proteomics analysis of differentially expressed proteins from ultraviolet-attenuated cercariae compared to normal cercariae

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Abstract

Schistosomiasis is considered the most important human helminthiasis in terms of morbidity and mortality. In this study, comparative soluble proteomic analysis of normal cercariae and ultraviolet-irradiated attenuated cercariae (UVAC) from Schistosoma japonicum were carried out in view of the high efficiency of irradiation-attenuated cercariae vaccine. The results revealed that some proteins showed significant differential expression in the parasite after treatment with ultraviolet light. Total 20 protein spots were identified by mass spectrometry, corresponded to five groups according to their functions in the main that were structural and motor proteins (actin, et al.), energy metabolism associated enzymes (glyceraldehydes-3-phosphage dehydrogenase, et al.), signaling transduction pathway-associated molecules (14-3-3 protein, et al.), heat shock protein families (HSP 70 family, et al.), and other functional proteins (20S proteasome). Furthermore, our results indicated that the differential expression of the proteins by ultraviolet irradiation may be, at least partially, acquired by regulating the mRNA levels of corresponding proteins. These results may provide new clues for further exploring the mechanism of protective immunity induced by UVAC and may shed some light on the development of vaccines against schistosomiasis.

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Acknowledgments

We gratefully thank Dr. Bernd Kalinna from the University of Melbourne for revising the manuscript. These experiments comply with the current laws of the China and supported by grants from the National Natural Science Foundation of China (grant no. 30771888), Specialized Research Fund for the Doctoral Program of Higher Education (grant no. 20050558069), China Postdoctoral Science Foundation (no. 20060390748), National High-Tech Research and Development Program of China (863 Program; grant no. 2006AA02Z444, 2007AA02Z153), and Project of Basic Platform for National Science and Technology Resources of the Ministry of Sciences and Technology of China (grant no. 2005 DKA21104).

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Authors and Affiliations

  1. Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 2nd Zhangshan Road, Guangzhou, 510089, China

    Lin-lin Yang, Zhi-yue Lv, Si-jie He, Zheng-yu Li, Shuang-min Zhang, Huan-qin Zheng, Xin-bing Yu & Zhong-dao Wu

  2. Key Laboratory for Tropical Diseases Control, The Ministry of Education, Sun Yat-sen University, Guangzhou, 510089, China

    Lin-lin Yang, Zhi-yue Lv, Si-jie He, Zheng-yu Li, Shuang-min Zhang, Huan-qin Zheng, Xin-bing Yu & Zhong-dao Wu

  3. Department of Biology, The Chinese University of Hong Kong, Hong Kong, China

    Shao-min Hu & Ming-Chiu Fung

  4. Proteomics Laboratory, Zhongshan School of Medicine, Sun Yat-sen University, 74 2nd Zhongshan road, Guangzhou, 510089, China

    Ming-tao Li

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  1. Lin-lin Yang
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Correspondence to Zhong-dao Wu.

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Lin-lin Yang and Zhi-yue Lv made the same contribution to this work.

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Yang, Ll., Lv, Zy., Hu, Sm. et al. Schistosoma japonicum: proteomics analysis of differentially expressed proteins from ultraviolet-attenuated cercariae compared to normal cercariae. Parasitol Res 105, 237–248 (2009). https://doi.org/10.1007/s00436-009-1387-z

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  • DOI: https://doi.org/10.1007/s00436-009-1387-z

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