Abstract
Probable arthropod vectors of avian blood protozoa, Leucocytozoon lovati, were collected in the alpine regions of Japan, the habitats of the host birds of Japanese rock ptarmigan (Lagopus mutus japonicus). Seven alpine regions of Japan, Asahidake, Chogatake, Tateyama, Jiigatake, Norikura, Kitadake, and Senjyogatake were investigated for black fly collection during 2004 to 2007. The collected 490 insects were morphologically identified as six species of female black flies, including Prosimulium hirtipes group (n = 59), Prosimulium mutata (n = 13), Prosimulium yezoense (n = 10), Similium japonicum (n = 359), Similium uchidai (n = 39), and Twinnia japonensis (n = 10). Extracted DNAs from individual black fly species were utilized for the amplification of the partial mitochondrial cytochrome b gene sequences of Leucocytozoon lovati previously reported. Four S. japonicum, two S. uchidai, and two P. hirtipes group studied were positive for the nested PCR among 490 black flies collected (1.6%; 8/490). All amplified sequences from the black flies were completely identical to those of L. lovati previously detected from Japanese rock ptarmigan. Our results suggest that at least three species of black flies, S. japonicum, S. uchidai, and P. hirtipes group, studied in this area could be regarded as potential vectors for L. lovati in the rock ptarmigan. This is the first detection case of Leucocytozoon from black flies of Japan.
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Acknowledgments
This study was partially supported by a Grant-in-Aid for Scientific Research (No. 16510179) from the Japan Society for the Promotion of Science, the Academic Frontier Project “Surveillance and control for zoonoses” and “High-Tech Research Center” Project for Private Universities from MEXT of Japan, Nihon University Joint Research Grant for YS, MY and KM (2007) and Global Environment Research Fund of the Ministry of the Environment of Japan (F-062) for YS and KM.
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Sato, Y., Tamada, A., Mochizuki, Y. et al. Molecular detection of Leucocytozoon lovati from probable vectors, black flies (Simuliudae) collected in the alpine regions of Japan. Parasitol Res 104, 251–255 (2009). https://doi.org/10.1007/s00436-008-1183-1
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DOI: https://doi.org/10.1007/s00436-008-1183-1