Abstract
The present paper describes the field evaluation of local gametocyte vaccine and its comparative efficacy with commercial anticoccidial vaccine, LivaCox®, used in breeder and broiler flocks in Pakistan. Humoral immune response in vaccinated and control chickens was monitored by enzyme-linked immunosorbent assay. Results demonstrated significantly elevated antibody titres in vaccinated groups as compared to control groups conducted both in Laboratory and field experiments. Significantly (P < 0.01) higher antibody titres in local gametocyte-vaccinated group as compared to LivaCox®-vaccinated chickens were recorded. Splenic cell migration inhibition assay was used to detect the cell-mediated immune (CMI) response, and results were expressed in terms of per cent migration index. Lower per cent migration index in LivaCox®-vaccinated chickens indicated the higher CMI response, as compared to local gametocyte-vaccinated chickens, although the difference was statistically non-significant (P > 0.05). Results of the challenge studies in laboratory experiments revealed significantly higher (P < 0.05) oocyst count in LivaCox®-vaccinated group as compared to local gametocyte-vaccinated chickens.Maximum protection (75%) against mixed species of genus Eimeria was recorded in chickens vaccinated with gametocyte vaccines as compared to LivaCox®-vaccinated group. The mean body weight gains in chickens vaccinated with local gametocyte vaccine were significantly better (P < 0.05) than in chickens vaccinated with LivaCox® vaccine, both in laboratory and field experiments. Majority of the chickens (70–72%) in control group demonstrated severe lesions (3.0–4.0), while 20–26% chickens showed moderate lesions (2.0). On the other hand, local gametocyte- and LivaCox®-immunized chickens developed 78% and 85% mild to moderated lesions (1.0–2.0), respectively. Results of the present study provide a probable explanation for cross-protection induced by Eimeria tenella gametocyte vaccines against other species of genus Eimeria.
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The funds for this project were sponsored by Pakistan Science Foundation, Islamabad, Pakistan (project no. P-PU/Bio/347).
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Anwar, M.I., Akhtar, M., Hussain, I. et al. Field evaluation of Eimeria tenella (local isolates) gametocytes vaccine and its comparative efficacy with imported live vaccine, LivaCox®. Parasitol Res 104, 135–143 (2008). https://doi.org/10.1007/s00436-008-1171-5
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DOI: https://doi.org/10.1007/s00436-008-1171-5