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Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae)

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Abstract

Angiostrongylus costaricensis can infect several mollusks, and its migration route in intermediate hosts has been studied only in Sarasinula marginata. To verify the susceptibility of Omalonyx sp. as an intermediate host of A. costaricensis and to analyze the nematode migration route, individuals were infected with stage 1 larvae. Obtained stage 3 larvae were orally inoculated in mice, and after 30 days, adult worms and stage 1 larvae were recovered, demonstrating Omalonyx susceptibility and suitability to infection. To define the parasite migration routes, specimens of Omalonyx with 30 min, 1 h, 2 h, 4 h, 6 h, 8 h, 2 days, 5 days, 10 days, 12 days, 15 days, 20 days, 21 days, 25 days, 28 days, and 30 days of infection were fixed and serially sectioned. Histological sections were stained with hematoxylin–eosin. The results were compared to those described in S. marginata. Oral and cutaneous infections were noted. After the penetration, larvae were retained, mainly in the fibromuscular tissue, by hemocytes, or they spread to the whole organism through the circulation, following the anatomical structure of the vasculature. The perilarval hemocyte reaction in Omalonyx was more intense until stage 2 larva instar, decreasing in the presence of stage 3 larvae. Differences in some aspects of hemocyte reaction between S. marginata and Omalonyx exemplify interspecific peculiarities in snail response to the same parasite.

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Acknowledgments

We thank Iolanda de Olinda Pedro, Andréa Natividade da Silva, Alexandra M dos Anjos, Luciana S Souza, Luzia HP Barroso (Departamento de Patologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ), Sueleny Silva Ferreira Teixeira, Marcílio da Silva Tomaz, Vera Lúcia de Jesus Mariano, and Wanderley Alves da Silva (CPqRR, Fiocruz, Belo Horizonte, MG), for their technical assistance, and Daniel Coscarelli (Laboratório de Malacologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG) for drawing Fig. 4. Photography was by Lângia C. Montresor. This experiment complies with the current laws of Brazil.

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Authors and Affiliations

  1. Laboratório de Malacologia e Sistemática Molecular, Departamento de Zoologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    Lângia C. Montresor, Teofânia H. D. A. Vidigal & Karyne N. de Souza

  2. Departamento de Medicina Veterinária Preventiva, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil

    André A. Fernandes

  3. Laboratório de Helmintoses Intestinais, Centro de Pesquisas René Rachou, Fundação Oswaldo Cruz, Belo Horizonte, Minas Gerais, Brazil

    Cristiane L. G. F. Mendonça & Omar S. Carvalho

  4. Departamento de Patologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil

    Luzia F. G. Caputo, Ester M. Mota & Henrique L. Lenzi

Authors
  1. Lângia C. Montresor
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  2. Teofânia H. D. A. Vidigal
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  3. Cristiane L. G. F. Mendonça
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  5. Karyne N. de Souza
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  6. Omar S. Carvalho
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  7. Luzia F. G. Caputo
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  8. Ester M. Mota
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  9. Henrique L. Lenzi
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Correspondence to Lângia C. Montresor.

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Montresor, L.C., Vidigal, T.H.D.A., Mendonça, C.L.G.F. et al. Angiostrongylus costaricensis (Nematoda: Protostrongylidae): migration route in experimental infection of Omalonyx sp. (Gastropoda: Succineidae). Parasitol Res 103, 1339–1346 (2008). https://doi.org/10.1007/s00436-008-1138-6

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  • DOI: https://doi.org/10.1007/s00436-008-1138-6

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