Abstract
Leishmania major is the causative agent of zoonotic cutaneous leishmaniasis (ZCL) in which gerbils are the reservoir host. ZCL is of great public health importance in Iran. In the current investigation, nested polymerase chain reaction (PCR) protocols were used to amplify a region of the ribosomal RNA amplicon of Leishmania (ITS1-5.8S rRNA gene). The PCR assays detected L. major in three rodent species: Rhombomis opimus, Meriones lybicus and, for first time, Meriones persicus. L. major parasite was found in Natanz, Isfahan Province in the center of Iran in a focus of rural zoonotic cutaneous leishmaniasis. Four L. major infections were detected in R. opimus species, three in M. Lybicus, and two in M. persicus. All nine rodent infections of L. major were found to be the same haplotype based on the PCR detection and sequencing of parasite ITS-ribosomal DNA gene. In addition, also for the first time, the nested PCR assays detected Leishmania tropica only in one M. persicus. Allied to studies in country, the new findings mean that past conclusions about the reservoir of L. major in Iran must be treated with caution. Finding two Leishmania species in different rodent species as reservoir in Iran, therefore, careful molecular eco-epidemiological investigations will be an essential part of modeling the roles of different gerbil species in maintaining and spreading ZCL foci.
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References
Alvar J, Barker JR (2002) Molecular tools for epidemiological studies and diagnosis of leishmaniasis and selected other parasitic diseases. Trans R Soc Trop Med Hyg 96(Suppl. 1):S1–S250
Ardehali S, Rezaei R, Nadim A (1994) Leishmania parasites and leishmaniasis, 4th edn. Tehran Academic Press, Tehran (in Farsi)
Ardehali S, Moattari A, Hatam GR, Hosseini SMH, Sharifi I (2000) Characterization of Leishmania isolate in Iran: serotyping with species specific monoclonal antibodies. Acta Trop 75:301–307
Azizi K, Rassi Y, Javadian E, Motazedian MH, Rafizadeh S, Yaghoobi-Ershadi MR, Mohebali M (2006) Phlebotomus (Paraphlebotomus) alexandri: a probable vector of Leishmania infantum in Iran. Ann Trop Med Parasitol 100:63–68
Boitani L, Bartoli S (1980) Macdonald encyclopedia of mammals, 1st edn. Macdonald & Co., London
Cupolillo E, Grimaldi, Momen H, Beverly SM (1995) Intergenic region typing (IRT): a rapid molecular approach to the characterization and evolution of Leishmania. Mol Biochem Parasitol 73:145–155
Dowlati Y, Firooz A (1997) Leishmaniasis (letter). J Am Acad Dermatol 37:139–140
Edrissian GH (1996) Visceral leishmaniasis in Iran and the role of serological tests in diagnosis and epidemiological studies. In: Ozel A, Ziyal lkass M (eds) Parasitology for the twenty first century. CAB International, Wallingford, pp 63–78
Eliseeve LN, Sidorova GA (1958) The course of cutaneous leishmaniasis in the great gerbil. Trudy Uzbekistanskogo Instituta Malyarii Meditisinkoi Parazitologii 3:61–79 (in Russian)
Eliseeve LN, Strelkova MV, Zherikhina II (1991) Features of epidemic activization of natural foci of zoonotic cutaneous leishmaniasis on place of Leishmania major, L. turanica and L. gerbilli sympatric dissemination. Med Parazitol 3:24–29 (in Russian)
Javadian E, Seyedi-Rasti MA (1991) Sandflies and their leptomonad infection in Iran. Parassitologia 33(Suppl):5
Killick-Kendrick R (1990) Phlebotomine vectors of the leishmaniases: a review. Med Vet Entomol 4:1–24
Killick-Kendrick R, Ward RD (1981) Ecology of the Leishmania. Parasitology 82:143–152
Mahboudi F, Abolhassani M, Yaran M, Mobtaker H, Azizi M (2001) Identification and differentiation of Iranian Leishmania species by PCR amplification of kDNA. Scand J Infect Dis 33:596–598
Mohebali M, Yaghoobi-Ershadi MR, Akhavan AA, Hajjaran H, Abaei MR (2004) Characterization of Leishmania infection in rodents from endemic areas of the Islamic Republic of Iran. East Mediterr Health J 10:591–599
Momeni Brojeni A (1994) Cutaneous leishmaniasis. In: Ardehali S, Rezaei R, Nadim A (eds) Leishmania parasites and leishmaniasis. Tehran Academic Press, Tehran, pp 46–68
Nadim A (2000) Leishmaniasis. In: Azizi F (ed) Epidemiology and control of prevalent disease in Iran. Endocrine and metabolism research center. 2nd edn. Tehran Academic Press, Tehran, pp 524–534
Nadim A, Faghih M (1968) The epidemiology of cutaneous leishmaniasis in the Isfahan province of Iran. I. The reservoir. II. The human disease. Trans R Soc Trop Med Hyg 61:534–542
Nadim A, Seyedi-Rashti MA (1971) A brief review of the epidemiology of various types of leishmaniasis in Iran. Acta Med Iran 14:99–106
Nadim A, Mesghali A, Amini H (1968a) Epidemiology of cutaneous leishmaniasis in the Isfahan province of Iran III. The vector. Trans R Soc Trop Med Hyg 62:543–549
Nadim A, Seyedi-Rashti MA, Mesgali A (1968b) Epidemiology of cutaneous leishmaniasis in Turkemen Sahara Iran. J Trop Med Hyg 71:238–239
Nasseri M, Modabber F (1977) Generalized infection and lack of delay hypersensitivity in Balb/C mice infection with Leishmania major. Presented at 8th Pahlavi Medical Congress, Shiraz, Iran
Parvizi P, Ready PD (2008) Nested PCRs of nuclear ITS-rDNA fragments detect three Leishmania species of gerbils in sanflies from Iranian Foci of zoonotic cutaneous leishmaniasis. Trop Med Int Health 13:1159–1171
Parvizi P, Mauricio I, Aransay AM, Miles MA, Ready PD (2005) First detection of Leishmania major in peridomestic Iranian sandflies: comparison of nested PCR of nuclear ITS ribosomal DNA and semi-nested PCR of minicircle kinetoplast DNA. Acta Trop 93:75–83
Ready PD, Lainson R, Shaw JJ, Souza AA (1991) DNA probes for distinguishing Psychodopygus wellcomei from Psychodopygus complexus (Diptera:Psychodidae). Mem Inst Oswaldo Cruz 86:41–49
Schönian G, El Fari M, Lewin S, Schweynoch C, Presber W (2001) Molecular epidemiology and population genetics in Leishmania. Med Microbiol Immunol 190:61–63
Shishlyaeva-Motova ZS (1966) Duration of cutaneous leishmaniasis in Rhombomys opimus Licht. and its dependence upon the season in which the animals acquire the infection. Med Parazitol 1:85–91 (in Russian)
Strelkova MV, Eliseev LN, Ponirovsky EN, Dergacheva TI, Evans DA (2001) Mixed leishmanial infections in Rhombomys optimus: a key to the persistence of Leishmania major from one transmission season to the next. Ann Trop Med Parasitol 95:811–819
Swofford DL (2002) PAUP*: phylogenetic analysis using parsimony (* and other methods) version 4.0. Sinauer, Sunderland, MA
Tashakori M, Kuhls K, Al-Jawabreh A, Mauricio I, Schönian G, Farajnia S, Alimohamadian MH (2006) Leishmania major: genetic heterogeneity of Iranian isolates by single-strand conformation polymorphism and sequence analysis of ribosomal DNA internal transcribed spacer. Acta Trop 98:52–58
Yaghoobi-Ershadi MR, Javadian E, Kannani A (1995a) Host preference pattern of Phlebotomine sandflies of Borkhar rural district, Isfahan Province, Iran. Acta Trop 60:155–158
Yaghoobi-Ershadi MR, Javadian E, Tahvildare-Bidruni GH (1995b) Leishmania major MON-26 isolated from naturally infected Phlebotomus papatasi (Diptera: Psychodidae) in Isfahan Province, Iran. Acta Trop 59:279–282
Yaghoobi-Ershadi MR, Akhavan AA, Mohebali M (1996) Meriones libycus and Rhombomys opimus (Rodentia: Gerbillidae) are the main reservoir hosts in a new focus of zoonotic cutaneous leishmaniasis in Iran. Trans R Soc Trop Med Hyg 90:503–504
Yaghoobi-Ershadi MR, Akhavan AA, Zahraei-Ramazani AR, Jalali-Zand AR, Piazak N (2005) Bionomics of Phlebotomus papatasi (Diptera: Psychodidae) in an endemic focus of zoonotic cutaneous leishmaniasis in central Iran. J Vector Ecol 30:115–118
Ziaei H (1996) A field guide for identifying of Iranian desert mammalians, 1st edn. Iranian Environment Organization, Tehran
Acknowledgments
The work was supported by the Pasteur Institute of Iran grant 263 awarded to Dr. Parviz Parvizi. The collections of sandflies were made possible by the assistance of the Centre of Health Service in Natanz, Isfahan Province. We thank Mehdi Baghban for help with the field work.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s00436-008-1220-0
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Parvizi, P., Moradi, G., Akbari, G. et al. PCR detection and sequencing of parasite ITS-rDNA gene from reservoirs host of zoonotic cutaneous leishmaniasis in central Iran. Parasitol Res 103, 1273–1278 (2008). https://doi.org/10.1007/s00436-008-1124-z
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DOI: https://doi.org/10.1007/s00436-008-1124-z