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Pulsed-UV light inactivation of Cryptosporidium parvum

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Abstract

Cryptosporidium parvum is an organism that threatens public health in the water industry. It is critical to develop improved detection methods as well as disinfection methods for protecting against cryptosporidiosis, which is caused by C. parvum. In this study, we investigated the ability of pulsed-light irradiation at 200–900 nm to inactivate C. parvum. Absolute quantitative real-time PCR was performed with cDNA made from total RNA extracted from C. parvum oocysts or HCT-8 cells infected with C. parvum oocysts in vitro. C. parvum oocysts in 100-mL quartz flasks were positioned 20, 30, and 40 cm from the light source, and the duration of irradiation was either 5 or 60 s. The reductions in oocyst viability (4.9 log10) and infectivity (6 log10) were maximal when the C. parvum oocysts were irradiated 20 cm from the pulsed-light source for 60 s, for which the UV dose was 278 mJ/cm2. The minimum dose of pulsed-UV light required for effective reduction in C. parvum infectivity (2 log10) was 15 mJ/cm2, which was achieved by 5 s of irradiation at 30 cm from the light source. This study confirmed that short-duration pulsed-UV light is an effective disinfection measure for C. parvum.

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Acknowledgment

This paper was supported by the Korea Ministry of Environment as “The Eco-technopia 21 project” in 2006 and by the program of Basic Atomic Energy Research Institute (BAERI), which is a part of the Nuclear R & D Programs funded by the Ministry of Science & Technology (MOST) of Korea in 2007.

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Authors and Affiliations

  1. Department of Environmental and Tropical Medicine, Konkuk University School of Medicine, Chungju, 380-701, Republic of Korea

    Soo-Ung Lee, Migyo Joung & Jae-Ran Yu

  2. En-Bio R&D Technology Center, Green EnTech Co., 153-787, Seoul, Republic of Korea

    Dong-Jin Yang & Soon-Ho Park

  3. Department of Parasitology, College of Medicine, Hallym University, Chuncheon, 200-702, Republic of Korea

    Sun Huh

  4. Department of Radiation Oncology, College of Medicine, Chungbuk National University, Cheongju, 361-763, Republic of Korea

    Woo-Yoon Park

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  1. Soo-Ung Lee
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  2. Migyo Joung
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  3. Dong-Jin Yang
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  4. Soon-Ho Park
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  5. Sun Huh
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  7. Jae-Ran Yu
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Correspondence to Jae-Ran Yu.

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Lee, SU., Joung, M., Yang, DJ. et al. Pulsed-UV light inactivation of Cryptosporidium parvum . Parasitol Res 102, 1293–1299 (2008). https://doi.org/10.1007/s00436-008-0908-5

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  • DOI: https://doi.org/10.1007/s00436-008-0908-5

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