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Cost-effective method of DNA extraction from taeniid eggs

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Abstract

A new cost-effective method using silicon dioxide- and guanidine isothiocyanate-containing buffers, after previous alkaline lysis, was established for the DNA extraction from taeniid eggs isolated from canine faeces. The purified DNA can be used to amplify the species-specific 12S mitochondrial DNA of Echinococcus multilocularis in direct and nested polymerase chain reaction in order to differentiate between E. multilocularis and Taenia spp.

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Authors and Affiliations

  1. Institute of Parasitology, University of Leipzig, An den Tierkliniken 35, 04103, Leipzig, Germany

    V. Dyachenko

  2. Institute of Biochemistry, Faculty of Medicine, Justus Liebig University Giessen, Friedrichstrasse 24, 35392, Giessen, Germany

    E. Beck

  3. Vet Med Laboratory GmbH, Division of IDEXX Laboratories, Mörikestrasse 28/3, 71636, Ludwigsburg, Germany

    N. Pantchev

  4. Institute of Parasitology, Justus Liebig University Giessen, Rudolf-Buchheim-Strasse 2, 35392, Giessen, Germany

    C. Bauer

Authors
  1. V. Dyachenko
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  2. E. Beck
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  3. N. Pantchev
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  4. C. Bauer
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Correspondence to V. Dyachenko.

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Dyachenko, V., Beck, E., Pantchev, N. et al. Cost-effective method of DNA extraction from taeniid eggs. Parasitol Res 102, 811–813 (2008). https://doi.org/10.1007/s00436-007-0855-6

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  • DOI: https://doi.org/10.1007/s00436-007-0855-6

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