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TSOL18/HP6-Tsol, an immunogenic Taenia solium oncospheral adhesion protein and potential protective antigen

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Abstract

In this study, we employed Taenia solium mRNA extracted from a tapeworm of Venezuelan origin to clone express and test the recombinant protein of the T. solium homologue of the 18-kDa oncospheral adhesion molecule of Taenia saginata (HP6-Tsag/TSA18). We first confirm the conserved nature of the sequence of the T. solium homologue (TSOL18/HP6-Tsol) and demonstrate that the recombinant protein, which, as with its T. saginata homologue, is characterised by a fibronectin type III homology region, functions as an adhesion molecule. This emphasises the possible importance of TSOL18/HP6-Tsol in tissue invasion, thus providing a rational explanation for its efficacy as a vaccine. As protection against Taenia spp., oncospheres is antibody mediated, logically, therefore, TSOL18/HP6-Tsol may also serve as a diagnostic antigen, as is indeed the case for recombinant HP6-Tsag/TSA18.

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Acknowledgements

This investigation received financial support from EU/INCO-DC (project CT95-0002), ISCIII-Programa Intramural (MPY 1274/05), ISCIII-RETIC RD06/0021/0019/0016, the Spanish Ministry of Science and Technology SAF2005-00362 and the Consejo de Desarrollo Científico y Humanístico of the Universidad de Carabobo. L. M. Gonzalez was sponsored by fellowships from the ISCIII, RICET-FIS and ISCIII-RETIC RD06/0021/0019 and E. Ferrer by fellowships from RECI, ISCIII and RICET-FIS. We would like to thank nurse C. González of Valle del Rio Hospital Ambulatorio and Dr. Eduardo Borges from the Venezuelan Ministry of Health for their help in the care and treatment of the hospital patients, Lic. Iris Dávila, Glenda Rojas, Yenny Alviares, Maria A. Pieters, Alejandro Aguilar and TSU Maria Lares, Argenis Molina and Jonathan Pérez for their technical support and our students, I. Delgado, N. Depinay, S. Linares and I. Yanez for helping with the field work. The experiments described in this paper comply with the current laws of the countries in which the work was performed.

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Authors and Affiliations

  1. Centro de Investigaciones de Enfermedades Tropicales (CIET), Facultad de Ciencias de la Salud, Universidad de Carabobo, San Carlos, Estado Cojedes, Venezuela

    Cruz M. Aguilar

  2. Centro de Biología Molecular, Universidad Autónoma de Madrid, 28049, Madrid, Spain

    Pedro Bonay

  3. Departamento de Parasitología and Instituto de Investigaciones Biomédicas (BIOMED), Universidad de Carabobo, Maracay, Venezuela

    Elizabeth Ferrer & Milagros M. Cortez A.

  4. Instituto de Salud Carlos III, Centro Nacional de Microbiología, Ctra. Majadahonda Pozuelo Km 2,2, 28220, Majadahonda, Madrid, Spain

    Luis Miguel González, Elizabeth Ferrer & Teresa Gárate

  5. University of Edinburgh, Royal (Dick) School of Veterinary Studies, Division of Veterinary Clinical Sciences, Centre for Tropical Veterinary Medicine, Easter Bush Veterinary Centre, Easter Bush, Roslin, Midlothian Scotland, UK, EH25 9RG

    Leslie J. S. Harrison

  6. Instituto Gulbenkian de Ciencia, Rua Quinta Grande 6, Apartado 14, P-2780-156, Oeiras Codex, Portugal

    R. Michael E. Parkhouse

Authors
  1. R. Michael E. Parkhouse
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  2. Pedro Bonay
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  3. Luis Miguel González
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  4. Elizabeth Ferrer
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  5. Teresa Gárate
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  6. Cruz M. Aguilar
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  7. Milagros M. Cortez A.
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  8. Leslie J. S. Harrison
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Corresponding authors

Correspondence to R. Michael E. Parkhouse or Leslie J. S. Harrison.

Additional information

The nucleotide sequence data for the molecule described in this paper is available in the GenBank, EMBL and DDBJ databases under the accession numbers AM774400, AJ581299, X97000 and X95983.

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Parkhouse, R.M.E., Bonay, P., González, L.M. et al. TSOL18/HP6-Tsol, an immunogenic Taenia solium oncospheral adhesion protein and potential protective antigen. Parasitol Res 102, 921–926 (2008). https://doi.org/10.1007/s00436-007-0853-8

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  • DOI: https://doi.org/10.1007/s00436-007-0853-8

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