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Development of Angiostrongylus costaricensis Morera and Céspedes 1971 (Nematoda: Angiostrongylidae) larvae in the intermediate host Sarasinula marginata (Semper 1885) (Mollusca: Soleolifera)

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Abstract

In life cycle of Angiostrongylus costaricensis, veronicellidae mollusks participate as the invertebrate host while rodents as the main vertebrate host. The current work shows a sequential larval development of A. costaricensis in Sarasinula marginata, individually killed and digested from day 1 to 43, post infection. Some larvae, recovered from sedimentation, were submitted to selective staining after paraffin embedded or inclusion in JB-4 to study inner structures. As control, four slugs were used, two killed at the beginning of infection and the others at the end of the experiment. At day 2 post infection, larvae were motionless and thick, presenting initial retention of granules. At day 4, L2 were detected, persisting until 43 days post infection. Larvae L2 displayed a large amount of granules rich in lipids and carbohydrates through its overall body, with more accumulation at the medial third corresponding to the esophagus–intestine transition site. Lipid granules, the main energetic source, were located at the basal and apical regions of intestinal cells. Both L1 and L3 presented bilateral alae, which is also common in other nematodes. Transition forms between L2 to L3 molts were also observed.

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Authors and Affiliations

  1. Laboratory of Intestinal Helminthiasis, Centro de Pesquisa René Rachou/Fiocruz, Avenida Augusto de Lima 1715, CEP 30190.002, Belo Horizonte, Minas Gerais, Brazil

    Cristiane L. G. F. Mendonça & Omar S. Carvalho

  2. Department of Pathology, Instituto Oswaldo Cruz/Fiocruz, Av Brasil 4365, CEP 21040.900, Rio de Janeiro, Rio de Janeiro, Brazil

    Ester M. Mota & Henrique L. Lenzi

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  1. Cristiane L. G. F. Mendonça
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  2. Omar S. Carvalho
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  3. Ester M. Mota
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  4. Henrique L. Lenzi
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Correspondence to Omar S. Carvalho.

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Mendonça, C.L.G.F., Carvalho, O.S., Mota, E.M. et al. Development of Angiostrongylus costaricensis Morera and Céspedes 1971 (Nematoda: Angiostrongylidae) larvae in the intermediate host Sarasinula marginata (Semper 1885) (Mollusca: Soleolifera). Parasitol Res 102, 861–865 (2008). https://doi.org/10.1007/s00436-007-0834-y

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  • DOI: https://doi.org/10.1007/s00436-007-0834-y

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