Abstract
Clonorchis sinensis, which causes clonorchiasis, is of major socioeconomic importance in China. In this study, we report the use of CotC, a major component of the Bacillus subtilis spore coat, as a fusion partner for the expression of C. sinensis TP20.8 (Tegumental Protein 20.8kDa) on the spore coat. Western blotting was used to identify TP20.8 surface expression on spores. Recombinant spores displaying the TP20.8 antigen were used for oral immunization and were shown to generate mucosal response in rats. TP20.8-specific secretory IgA in feces reached significant levels 2 weeks after oral dosing. This report shows that surface display of recombinant C. sinensis TP20.8 on B. subtilis spores was immunogenic and B. subtilis spores can be used as a mucosal immunization vehicle for parasite prevention and control.
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Acknowledgments
We thank Professor Fung Ming Chiu (Department of Biology, the Chinese University of Hong Kong) for his help in revising the manuscript. This work was supported by grants from the National Natural Science Foundation of China (no. 30671831) and the National High Technology Research and Development Program of China (863 program; no. 2006AA02Z422).
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Zhou, Z., Xia, H., Hu, X. et al. Immunogenicity of recombinant Bacillus subtilis spores expressing Clonorchis sinensis tegumental protein. Parasitol Res 102, 293–297 (2008). https://doi.org/10.1007/s00436-007-0762-x
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DOI: https://doi.org/10.1007/s00436-007-0762-x