Abstract
In a previous study, we reported that the novel annexin XX1 (annexin E1), identical to α14-giardin, is specifically localized to the flagella and to the median body of the trophozoites. However, the mode of interaction and the direct partners involved remained unclear. In the present study, we show that α4-giardin obviously does not evenly distribute over the full length of the axonemes, but rather, resides at local slubs near the proximal part and the ends of the flagella. In immunocytochemical co-localization studies, the anti-giardin primary antibody exclusively reacted with distinct regions of the flagella in permeabilized cells, whereas the anti-tubulin antibody bound to all areas of the axonemes in the cells and to isolated cytoskeletons. Isolated cytoskeletons did not react with anti-giardin antibodies. α14-Giardin itself is able to assemble to multimeric structures. Taken together, our findings suggest that α14-giardin adheres to microtubules of the flagella via self-assembly that may regulated by Ser/Thr-phosphorylation.
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Acknowledgement
The authors thank Dr. Jan Hegemann (European Neuroscience Institute, Göttingen) for valuable advice in electron microscopy and Dr. Stefan Walter for performing ESI-MS. This work includes parts of the doctoral thesis of A.V., who is a recipient of a grant from the research training group 612 of the Deutsche Forschungsgemeinschaft.
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An erratum to this article can be found at http://dx.doi.org/10.1007/s00436-007-0814-2
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Vahrmann, A., Šarić, M., Scholze, H. et al. α14-Giardin (annexin E1) is associated with tubulin in trophozoites of Giardia lamblia and forms local slubs in the flagella. Parasitol Res 102, 321–326 (2008). https://doi.org/10.1007/s00436-007-0758-6
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DOI: https://doi.org/10.1007/s00436-007-0758-6