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Immune response and inhibitory effect of ketotifen on the BALB/c and C3H/HeN mice infected with Echinostoma hortense

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Abstract

Although Echinostoma hortense is one of the intestinal trematodes with a high infection rate in South Korea, the exact immune response against E. hortense infection has yet to be fully investigated. In the present study, we investigated differential susceptibilities in two different strains of micenamely, BALB/c (H-2d) and C3H/HeN (H-2k) mice. Likewise, we investigated the effects of ketotifen, an antiallergic drug, on the immune response against E. hortense infection. The worm recovery rate of the C3H/HeN mice was much higher than that of the BALB/c mice. The messenger ribonucleic acid (mRNA) expressions of interleukin (IL)-4 and IL-5 in the BALB/c mice were stronger than that of the C3H/HeN mice after E. hortense infection, but IL-1β and tumor necrosis factor (TNF)-α expressions in the BALB/c mice were weaker than that of the C3H/HeN mice after E. hortense infection. The number of goblet cells and eosinophils increased after E. hortense infection in the BALB/c and the C3H/HeN mice. The worm recovery rate was higher and lasted longer in the ketotifen-treated mice in comparison to the untreated mice. Ketotifen suppressed the mRNA expression of IL-4 and IL-5 in the BALB/c mice, but did not in the C3H/HeN mice. The IL-1β expressions were inhibited by ketotifen in the two strains, but TNF-α expression was inhibited in the C3H/HeN mice after ketotifen treatment. In addition, ketotifen inhibited the increase in eosinophils and goblet cells in varying degrees, depending on the strain. In summary, the immune sensitivity against E. hortense depends on the species of the host. The ketotifen treatment administered on the infected mice differently blocked the immune response against E. hortense infection.

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Acknowledgment

This study was supported by a grant from the Maeji Institute of Academic Research in the fiscal year 2004.

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Authors and Affiliations

  1. Department of Biomedical Laboratory Science, Institute of Health Science, College of Health Science, Yonsei University, Wonju, 220-701, Republic of Korea

    Yong Suk Ryang, Eun Ju Yang, Jeong-Lye Kim, Ho-Joong Sung & Jong-Bae Kim

  2. Department of Parasitiology, the Institute of Basic Medical Science, Wonju College of Medicine, Yonsei University, Wonju, 220-701, Republic of Korea

    Kyu-Jae Lee

  3. Department of Biomedical Laboratory Science, School of Medicine, Eulji University, 143-5, Yeuongdu-dong, Jung-gu, Daejeon, 301-832, Republic of Korea

    In Sik Kim

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  1. Yong Suk Ryang
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  2. Eun Ju Yang
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  4. Kyu-Jae Lee
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Correspondence to In Sik Kim.

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Ryang, Y.S., Yang, E.J., Kim, JL. et al. Immune response and inhibitory effect of ketotifen on the BALB/c and C3H/HeN mice infected with Echinostoma hortense . Parasitol Res 101, 1103–1110 (2007). https://doi.org/10.1007/s00436-007-0591-y

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  • DOI: https://doi.org/10.1007/s00436-007-0591-y

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