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Anthelmintic resistance in trichostrongylid nematodes of sheep farms in Northwest Spain

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Abstract

A survey to determine the prevalence of anthelmintic resistance in nematode parasites of sheep against the three main families of anthelmintics (benzimidazoles, macrocyclic lactones and imidazothiazoles) was carried out from January 1999 to December 2003 involving 85 flocks in Northwest (NW) Spain. In the study on prevalence of resistance to benzimidazoles, faecal egg count reduction test (FECRT) showed there was resistance in 8 (12.7%) flocks, 1 (1.6%) was suspected, and 54 (85.7%) were susceptible. The results indicated that 9 flocks (34.61%) showed resistance, 1 (3.85%) showed suspected resistance, and 16 (61.54%) were susceptible to the imidazothiazoles. Against macrocyclic lactones, resistance was observed in 8 flocks (15.69%), 4 (7.84%) showed suspected resistance and 39 (76.47%) were susceptible. None of the flocks used in the study showed resistance to the three families of anthelmintics. Nevertheless, six were recorded as resistant or suspected of being resistant to two of these families of anthelmintics. Egg hatch assay (EHA) and FECRT were carried out jointly on 61 flocks, although EHA was done on a total of 83 farms. The results showed that 15 (18.07%) of the 83 flocks were resistant, with egg death 50 over 0.1 μg/ml thiabendazole, and 68 (81.93%) were susceptible to benzimidazoles. When the results between FECRT and EHA were compared, both techniques showed good correlation in field studies. Faecal cultures performed pre- and post-treatment indicated that Teladorsagia and Trichostrongylus were the main genera.

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References

  • Bartley DJ, Jackson E, Johnston K, Coop RL, Mitchell GB, Sales J, Jackson F (2003) A survey of anthelmintic resistant nematode parasites in Scottish sheep flocks. Vet Parasitol 117:61–71

    Article  PubMed  Google Scholar 

  • Borgsteede FH, Pekelder JJ, Dercksen DP, Sol J, Vellema P, Gaasenbeek CP, van der Linden JN (1997) A survey of anthelmintic resistance in nematodes of sheep in The Netherlands. Vet Q 19:167–172

    PubMed  CAS  Google Scholar 

  • Chandrawathani P, Adnan M, Waller PJ (1999) Anthelmintic resistance in sheep and goat farms on Peninsular Malaysia. Vet Parasitol 82:305–310

    Article  PubMed  CAS  Google Scholar 

  • Chartier C, Soubirac F, Pors I, Silvestre A, Hubert J, Couquet C, Cabaret J (2001) Prevalence of anthelmintic resistance in gastrointestinal nematodes of dairy goats under extensive management conditions in southwestern France. J Helminthol 75:325–330

    PubMed  CAS  Google Scholar 

  • Coles GC (1997) Nematode control practices and anthelmintic resistance on British sheep farms. Vet Rec 141:91–93

    PubMed  CAS  Google Scholar 

  • Coles GC, Bauer C, Borgsteede FHM, Geerts S, Klei TR, Taylor MA, Waller PJ (1992) World Association for the Advancement of Veterinary Parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Parasitol 44:35–44

    Article  PubMed  CAS  Google Scholar 

  • Conder GA, Campbell WC (1995) Chemotherapy of nematode infections of veterinary importance, with special reference to drug resistance. Adv Parasitol 35:1–84

    Article  PubMed  CAS  Google Scholar 

  • Craven J, Bjorn H, Barnes EH, Henriksen SA, Nansen P (1999) A comparison of in vitro tests and a faecal egg count reduction test in detecting anthelmintic resistance in horse strongyles. Vet Parasitol 85:49–59

    Article  PubMed  CAS  Google Scholar 

  • Echevarria F, Borba MFS, Pinheiro AC, Waller PJ, Hansen JW (1996) The prevalence of anthelmintic resistance in nematode parasites of sheep in southern Latin America: Brazil. Vet Parasitol 62:199–206

    Article  PubMed  CAS  Google Scholar 

  • Eddi C, Caracostantogolo J, Pena M, Schapiro J, Marangunich L, Waller PJ, Hansen JW (1996) The prevalence of anthelmintic resistance in nematode parasites of sheep in southern Latin America: Argentina. Vet Parasitol 62:189–197

    Article  PubMed  CAS  Google Scholar 

  • Hong C, Hunt KR, Coles GC (1996) Occurrence of anthelmintic resistant nematodes on sheep farms in England and goat farms in England and Wales. Vet Rec 139:83–86

    PubMed  CAS  Google Scholar 

  • Hunt RK, Taylor MA (1989) Use of the egg hatch on sheep faecal samples for the detection of benzimidazole resistant nematodes. Vet Rec 12:153

    Google Scholar 

  • Jackson F, Coop RL (2000) The development of anthelmintic resistance in sheep nematodes. Parasitol 120:S95–S107

    Article  Google Scholar 

  • Laha R, Hemaprasanth, Harbola PC (1999) Anthelmintic resistance in Pashmina (Cashmere) producing goats in India. Vet Res Commun 23:187–189

    Article  PubMed  CAS  Google Scholar 

  • Maciel S, Gimenez AM, Gaona C, Waller PJ, Hansen JW (1996) The prevalence of anthelmintic resistance in nematode parasites of sheep in southern Latin America: Paraguay. Vet Parasitol 62:207–212

    Article  PubMed  CAS  Google Scholar 

  • Maingi N, Bjorn H, Thamsborg SM, Bogh HO, Nansen P (1996) A survey of anthelmintic resistance in nematode parasites of goats in Denmark. Vet Parasitol 66:53–66

    Article  PubMed  CAS  Google Scholar 

  • Ministry of Agriculture, Fisheries and Food (MAFF) (1986) Manual of veterinary parasitological laboratory techniques. Reference book 418, HMSO, London

    Google Scholar 

  • Mitchell GBB, Jackson F, Coop RL (1991) Anthelmintic resistance in Scotland. Vet Rec 129:58

    CAS  PubMed  Google Scholar 

  • Nari A, Salles J, Gil A, Waller PJ, Hansen JW (1996) The prevalence of anthelmintic resistance in nematode parasites of sheep in southern Latin America: Uruguay. Vet Parasitol 62:213–222

    Article  PubMed  CAS  Google Scholar 

  • Papadopoulos E, Himonas C, Coles GC (2001) Drought and flock isolation may enhance the development of anthelmintic resistance in nematodes. Vet Parasitol 97:253–259

    Article  PubMed  CAS  Google Scholar 

  • Requejo-Fernández JA, Martínez A, Meana A, Rojo-Vázquez FA, Osoro K, Ortega-Mora LM (1997) Anthelmintic resistance in nematode parasites from goats in Spain. Vet Parasitol 73:83–88

    Article  PubMed  Google Scholar 

  • Samson-Himmelstjerna G, von Witzendorff C, Sievers G, Schnieder T (2002) Comparative use of faecal egg count reduction test, egg hatch assay and beta-tubulin codon 200 genotyping in small strongyles (cyathostominae) before and after benzimidazole treatment. Vet Parasitol 108:227–235

    Article  PubMed  Google Scholar 

  • Swarnkar CP, Sanyal PK, Singh D, Khan FA, Bhagwan PS (2001) Anthelmintic resistance on an organized sheep farm in India. Trop Anim Health Prod 33:305–312

    Article  PubMed  CAS  Google Scholar 

  • Terrill TH, Kaplan RM, Larsen M, Samples OM, Miller JE, Gelaye S (2001) Anthelmintic resistance on goat farms in Georgia: efficacy of anthelmintics against gastrointestinal nematodes in two selected goat herds. Vet Parasitol 97:261–268

    Article  PubMed  CAS  Google Scholar 

  • Torres-Acosta JF, Dzul-Canche U, Aguilar-Caballero AJ, Rodríguez-Vivas RI (2003) Prevalence of benzimidazole resistant nematodes in sheep flocks in Yucatan, Mexico. Vet Parasitol 114:33–42

    Article  PubMed  CAS  Google Scholar 

  • Uhlinger C, Fleming S, Moncol D (1992) Survey for drug-resistant gastrointestinal nematodes in 13 commercial sheep flocks. J Am Vet Med Assoc 201:77–80

    PubMed  CAS  Google Scholar 

  • Van Wyk JA, Stenson MO, Van der Merwe JS, Vorster RJ, Viljoen PG (1999) Anthelmintic resistance in South Africa: surveys indicate an extremely serious situation in sheep and goat farming. Onderstepoort J Vet Res 66:273–284

    PubMed  Google Scholar 

  • Varady M, Praslicka J, Corba J (1995) The evaluation of in vivo faecal egg count reduction test and in vitro egg hatch test for detection of benzimidazole resistance in sheep nematodes. Helminthol 32:225–228

    CAS  Google Scholar 

  • Waller PJ (1997) Anthelmintic resistance. Vet Parasitol 72:391–412

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgement

This work has been partially funded by a grant from the Spanish Ministry of Science and Technology (project number AGF98-0203).

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Authors and Affiliations

  1. Dpt. Patología Animal (Sanidad Animal), Facultad de Veterinaria, Universidad de León., C/ Profesor Pedro Cármenes S/N, 24071, León, Spain

    M. A. Álvarez-Sánchez, J. Pérez-García, M. A. Cruz-Rojo & F. A. Rojo-Vázquez

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  1. M. A. Álvarez-Sánchez
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  2. J. Pérez-García
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  3. M. A. Cruz-Rojo
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  4. F. A. Rojo-Vázquez
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Correspondence to F. A. Rojo-Vázquez.

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Álvarez-Sánchez, M.A., Pérez-García, J., Cruz-Rojo, M.A. et al. Anthelmintic resistance in trichostrongylid nematodes of sheep farms in Northwest Spain. Parasitol Res 99, 78–83 (2006). https://doi.org/10.1007/s00436-006-0130-2

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