Skip to main content
Log in

Molecular identification and characterization of canine Hepatozoon species from Brazil

  • Original Paper
  • Published:
Parasitology Research Aims and scope Submit manuscript

Abstract

Canine Hepatozoon species from Brazil was molecular identified and characterized for the first time. From 31 dogs, 7 were positive for blood smear examination and 21 positive for PCR. Partial sequences of the 18S rRNA gene from eight naturally infected dogs were analyzed. Sequences revealed that Brazilian Hepatozoon is closely related with the Japanese Hepatozoon, that has 99% nucleotide identity with Hepatozoon canis from Israel, and different from Hepatozoon americanum. These results indicate that the canine Hepatozoon species from Brazil is H. canis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Baneth G, Barta JR, Shkap V, Martin DS, Macintire DK, Vincent-Johnson N (2000) Genetic and antigenic evidence supports the separation of Hepatozoon canis and Hepatozoon americanum at the species level. J Clin Microbiol 38:1298–1301

    CAS  PubMed  Google Scholar 

  • Baneth G, Mathew JS, Shkap V, Macintire DK, Barta JR, Ewing SA (2003) Canine hepatozoonosis: two disease syndromes caused by separate Hepatozoon spp. Trends Parasitol 19:27–31

    Article  PubMed  Google Scholar 

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Google Scholar 

  • Gondim LFP, Konayagawa A, Alencar NX, Biondo AW, Takahira RF, Franco SRV (1998) Canine hepatozoonosis in Brazil: description of eight naturally occurring cases. Vet Parasitol 74:319–323

    Article  CAS  PubMed  Google Scholar 

  • Inokuma H, Okuda M, Ohno K, Shimoda K, Onishi T (2002) Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs. Vet Parasitol 106:265–271

    Article  CAS  PubMed  Google Scholar 

  • Massard CA (1979) Hepatozoon canis (James, 1905) (Adeleida: Hepatozoidae) de cães do Brasil, com uma revisão do gênero em membros da ordem carnívora. Seropédica: UFRRJ, Departamento de Parasitologia (Tese, Mestrado) 121 p

  • Mathew JS, Van Den Bussche RA, Ewing AS, Malayer JR, Latha BR, Panciera RJ (2000) Phylogenetic relationships of Hepatozoon (Apicomplexa: Adeleorina) based on molecular, morphologic and life-cycle characters. J Parasitol 86:366–372

    CAS  PubMed  Google Scholar 

  • Mundim AV, Jacomini JO, Mundim MJS, Araújo SF (1992) Hepatozoon canis (James, 1905) em cães de Uberlândia, Minas Gerais. Relato de dois casos. Braz J Vet Res Anim Sci 29:259–261

    Google Scholar 

  • Mundim AV, Mundim MJS, Jensen NMP, Araújo SF (1994) Hepatozoon canis: estudo retrospectivo de 22 casos de infecção natural em cães de Uberlândia, MG. Rev Cent Ciênc Bioméd Univ Fed Uberlândia 10:89–95

    Google Scholar 

  • O’Dwyer LH, Massard CL, Pereira De Souza JC (2001) Hepatozoon canis infection associated with dog ticks of rural areas of Rio de Janeiro State, Brazil. Vet Parasitol 94:143–150

    Article  CAS  PubMed  Google Scholar 

  • O’Dwyer LH, Saito ME, Hasegawa MY, Kohayagawa A (2004) Tissue stages of Hepatozoon canis in naturally infected dogs from São Paulo State, Brazil. Parasitol Res 94:240–242

    Article  PubMed  Google Scholar 

  • Page RDM (1996) Tree view: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    CAS  PubMed  Google Scholar 

  • Paludo GR, Dell’Porto A, Castro e Trindade AR, Mcmanus C, Friedman H (2003) Hepatozoon spp.: report of some cases in dogs in Brasília, Brazil. Vet Parasitol 118:243–248

    Article  PubMed  Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Acids Res 22:4673–4680

    CAS  PubMed  Google Scholar 

  • Vincent-Johnson NA, Macintire DK, Lindsay DL, Lenz SD, Baneth G, Shkap V, Blagburn BL (1997) A new Hepatozoon species from dogs: description of the causative agent of canine hepatozoonosis in North America. J Parasitol 83:1165–1172

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Lucia Helena O’Dwyer.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Rubini, A.S., Paduan, K.d.S., Cavalcante, G.G. et al. Molecular identification and characterization of canine Hepatozoon species from Brazil. Parasitol Res 97, 91–93 (2005). https://doi.org/10.1007/s00436-005-1383-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00436-005-1383-x

Keywords

Navigation