Abstract
This study aimed to explore the metabolic consequences of cryptosporidiosis in an acute experimental model both at the peak of infection and after parasite clearance. Four-day-old suckling rats were infected with 106 oocysts of Cryptosporidium parvum . At the peak of infection (day 8 PI), C. parvum resulted in a dramatic reduction both in nutrient intake (−50%) and body weight (16.3±5.2 vs 27.3±1.0 g, P<0.01) with a decrease in both lean body mass and adipose tissue. Muscular fractional and absolute synthesis rate were reduced (−15 and −55%, respectively). After parasite clearance (day 17 PI), body weight remained reduced in formerly infected animals (37.8±8.0 vs 47.8±4.2 g, P<0.01) whereas nutrient intake normalized and fractional synthesis rate slightly increased (+22%) compared to controls. Overall, our results show that the impact and consequences of cryptosporidiosis are far greater than generally appreciated, leading to major malnutrition in suckling rats.
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Alcantara CS, Yang CH, Steiner TS, Barrett LJ, Lima AA, Chappell CL, Okhuysen PC, White AC Jr, Guerrant RL (2003) Interleukin-8, tumor necrosis factor-alpha, and lactoferrin in immunocompetent hosts with experimental and Brazilian children with acquired cryptosporidiosis. Am J Trop Med Hyg 68:325–328
Barbot L, Windsor E, Rome S, Tricottet V, Reynes M, Topouchian A, Huneau JF, Gobert JG, Tome D, Kapel N (2003) Intestinal peptide transporter PepT1 is over-expressed during acute cryptosporidiosis in suckling rats as a result of both malnutrition and experimental parasite infection. Parasitol Res 89:364–370
Breuille D, Rose F, Arnal M, Melin C, Obled C (1994) Sepsis modify the contribution of different organs to whole-body protein synthesis in rats. Clin Sci (Lond) 86:663–669
Capet C, Kapel N, Huneau JF, Magne D, Laikuen R, Tricottet V, Benhamou Y, Tome D, Gobert JG (1999) Cryptosporidium parvum infection in suckling rats: impairment of mucosal permeability and Na(+)-glucose cotransport. Exp Parasitol 91:119–125
Casemore DP (1990) Epidemiological aspects of human cryptosporidiosis. Epidemiol Infect 104:1–28
Checkley W, Gilman RH, Epstein LD, Suarez M, Diaz JF, Cabrera L, Black RE, Sterling CR (1997) Asymptomatic and symptomatic cryptosporidiosis: their acute effect on weight gain in Peruvian children. Am J Epidemiol 145:156–163
Checkley W, Epstein LD, Gilman RH, Black RE, Cabrera L, Sterling CR (1998) Effects of Cryptosporidium parvum infection in Peruvian children: growth faltering and subsequent catch-up growth. Am J Epidemiol 148:497–506
Dillingham RA, Lima AA, Guerrant RL (2002) Cryptosporidiosis: epidemiology and impact. Microbes Infect 4:1059–1066
Fayer R, Morgan U, Upton SJ (2000) Epidemiology of Cryptosporidium: transmission, detection and identification. Int J Parasitol 30:1305–1322
Garlick PJ, McNurlan MA, Preedy VR (1980) A rapid and convenient technique for measuring the rate of protein synthesis in tissues by injection of [3H]phenylalanine. Biochem J 192:719–723
Golden MH, Waterlow JC, Picou D (1977) Protein turnover, synthesis and breakdown before and after recovery from protein-energy malnutrition. Clin Sci Mol Med 53:473–477
Guerrant DI, Moore SR, Lima AA, Patrick PD, Schorling JB, Guerrant RL (1999) Association of early childhood diarrhea and cryptosporidiosis with impaired physical fitness and cognitive function four-seven years later in a poor urban community in northeast Brazil. Am J Trop Med Hyg 61:707–713
James WP (1972) Protein synthesis and breakdown and amino acid catabolism in protein-calorie malnutrition. Proc Nutr Soc 31:225–231
Kyriazakis I, Tolkamp BJ, Hutchings MR (1998) Towards a functional explanation for the occurrence of anorexia during parasitic infections. Anim Behav 56: 265–274
Lacroix S, Mancassola R, Naciri M, Laurent F (2001) Cryptosporidium parvum-specific mucosal immune response in C57BL/6 neonatal and gamma interferon-deficient mice: role of tumor necrosis factor alpha in protection. Infect Immun 69:1635–1642
Molbak K, Andersen M, Aaby P, Hojlyng N, Jakobsen M, Sodemann M, da Silva AP (1997) Cryptosporidium infection in infancy as a cause of malnutrition: a community study from Guinea-Bissau, west Africa. Am J Clin Nutr 65:149–152
Mosier DA, Oberst RD (2000) Cryptosporidiosis. A global challenge. Ann N Y Acad Sci 916:102–111
Topouchian A, Kapel N, Huneau JF, Barbot L, Magne D, Tome D, Gobert JG (2001) Impairment of amino-acid absorption in suckling rats infected with Cryptosporidium parvum. Parasitol Res 87:891–896
Topouchian A, Huneau JF, Barbot L, Rome S, Gobert JG, Tome D, Kapel N (2003) Evidence for the absence of an intestinal adaptive mechanism to compensate for C. parvum-induced amino acid malabsorption in suckling rats. Parasitol Res 91:197–203
Turk DE (1974) Intestinal parasitism and nutrient absorption. Fed Proc 33:106–111
Wykes LJ, Fiorotto M, Burrin DG, Del Rosario M, Frazer ME, Pond WG, Jahoor F (1996) Chronic low protein intake reduces tissue protein synthesis in a pig model of protein malnutrition. J Nutr 126:1481–1488
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Topouchian, A., Kapel, N., Larue-Achagiotis, C. et al. Cryptosporidium infection impairs growth and muscular protein synthesis in suckling rats. Parasitol Res 96, 326–330 (2005). https://doi.org/10.1007/s00436-005-1369-8
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DOI: https://doi.org/10.1007/s00436-005-1369-8