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Molecular characterization of Enterocytozoon bieneusi in cattle indicates that only some isolates have zoonotic potential

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An Erratum to this article was published on 30 March 2004

Abstract

In this study, 338 fecal samples were analyzed for Enterocytozoon bieneusi from cattle farms in Florida, Maryland, Michigan, New York, North Carolina, Pennsylvania, Virginia, and Portugal. The internal transcribed spacer region (392 bp) of the rRNA gene of E. bieneusi was amplified using a nested PCR protocol. Thirty-two E. bieneusi-PCR positive samples were sequenced. A high degree of genetic polymorphism, represented by five distinct genotypes (BEB1–BEB5), was found among the E. bieneusi isolates from cattle. Most of the isolates formed a distinct cluster consisting of only the four cattle genotypes, but six isolates of a genotype clustered together with E. bieneusi genotypes from humans and other domestic animals. Therefore, only some E. bieneusi isolates from cattle may be of public health importance.

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References

  • Breitenmoser AC, Mathis A, Burgi E, Weber R, Deplazes P (1999) High prevalence of Enterocytozoon bieneusi in swine with four genotypes that differ from those identified in humans. Parasitology 118:447–453

    Google Scholar 

  • Buckholt MA, Lee JH, Tzipori S (2002) Prevalence of Enterocytozoon bieneusi in swine: an 18-month survey at a slaughterhouse in Massachusetts. Appl Environ Microbiol 68:2595–2599

    Google Scholar 

  • Chalifoux LV, Carville A, Pauley D, Thompson B, Lackner AA, Mansfield KG (2000) Enterocytozoon bieneusi as a cause of proliferative serositis in simian immunodeficiency virus-infected immunodeficient macaques (Macaca mulatta). Arch Pathol Lab Med 124:1480–1484

    Google Scholar 

  • Cotte L, Rabodonirina M, Chapuis F, Bailly F, Bissuel F, Raynal C, Gelas P, Persat F, Piens M, Trepo C (1999) Waterborne outbreak of intestinal microsporidiosis in persons with and without immunodeficiency virus infection. J Infect Dis 180:2003–2008

    CAS  PubMed  Google Scholar 

  • Dengjel B, Zahler M, Hermanns W, Heinritzki K, Spillmann T, Thomschke A, Loscher T, Gothe R, Rinder H (2001) Zoonotic potential of Enterocytozoon bieneusi. J Clin Microbiol 39:4495–4499

    Google Scholar 

  • Deplazes P, Mathis A, Muller C, Weber R (1996) Molecular epidemiology of Encephalitozoon cuniculi and first detection of Enterocytozoon bieneusi in fecal samples of pigs. J Eukaryot Microbiol 43:93S

    CAS  PubMed  Google Scholar 

  • Desportes I, Le Charpentier Y, Galian A, Bernard F, Cochant-Priollet B, Lavergne A, Ravisse P, Modigliani R (1985) Occurrence of a new microsporidan: Enterocytozoon bieneusi n.g., n. sp., in the enterocytes of a human patient with AIDS. J Protozool 32:250–254

    CAS  PubMed  Google Scholar 

  • Dowd SE, Gerba CP, Pepper IL (1998) Confirmation in the human-pathogenic microsporidia Enterocytozoon bieneusi, Encepalitozoon intestinalis and Vittaforma corneae in water. Appl Environ Microbiol 64:3332–3335

    CAS  PubMed  Google Scholar 

  • Fayer R, Santin M, Trout JM (2003) First detection of microsporidia in dairy calves in North America. Parasitol Res 90:383–386

    Article  CAS  PubMed  Google Scholar 

  • Felsenstein J (1989) PHYLIP-phylogeny interference package (version 3.2). Cladistics 5:164–166

    Google Scholar 

  • Fournier SO, Liguory O, Santillana-Hyat M, Guillot E, Sarfati C, Dumoutier N, Molina J, Derouin F (2000) Detection of microspoidia in surface water: a one year follow-up study. FEMS Immun Med Microbiol 29:95–100

    Article  CAS  Google Scholar 

  • Franzen C, Muller A (1999) Molecular techniques for detection, species differentiation, and phylogenetic analysis of microsporidia. Clin Microbiol Rev 12:243–285

    CAS  PubMed  Google Scholar 

  • Gool T van, Luderhoff E, Nathoo KJ, Kiire CF, Dankert J, Mason PR (1995) High prevalence of Enterocytozoon bieneusi infections among HIV-positive individuals with persistent diarrhea in Harare, Zimbabwe. Trans R Soc Trop Med Hyg 89:478–480

    PubMed  Google Scholar 

  • Liguory O, David F, Sarfati C, Derouin F, Molina JM (1998) Determination of types of Enterocytozoon bieneusi strain isolated from patients with intestinal microsporidiosis. J Clin Microbiol 36:1882–1885

    CAS  PubMed  Google Scholar 

  • Lores B, Lopez-Miragaya I, Arias C, Fenoy S, Torres J, del Aguila C (2002) Intestinal microsporidiosis due to Enterocytozoon bieneusi in elderly human immunodeficiency virus-negative patients from Vigo, Spain. Clin Infect Dis 34:918–921

    Article  PubMed  Google Scholar 

  • Mathis A, Breitenmoser AC, Deplazes P (1999) Detection of new Enterocytozoon genotypes in fecal samples of farm dogs and a cat. Parasite 6:189–193

    Google Scholar 

  • Peer Y van de , Wachter R de (1994) TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10:569–570

    PubMed  Google Scholar 

  • Reetz J, Rinder H, Thomschke A, Manke H, Schwebs M, Bruderek A (2002) First detection of the microsporidium Enterocytozoon bieneusi in non-mammalian hosts (chickens). Int J Parasitol 32:785–787

    Google Scholar 

  • Rinder H, Katzwinkel-Wladarsch S, Loscher T (1997) Evidence for the existence of genetically distinct strains of Enterocytozoon bieneusi. Parasitol Res 83:670–672

    CAS  PubMed  Google Scholar 

  • Rinder H, Thomschke A, Essig A, Heinritzi K, Gothe R, Zahler M (1999) Mikrosporidien bei Schweinen mit lebensbedrohlicher Diarrhö. Tierarztl Prax 27:231–234

    Google Scholar 

  • Rinder H, Thomschke A, Dengjel B, Gothe R, Loscher T, Zahler M (2000) Close genotypic relationship between Enterocytozoon bieneusi from humans and pigs and first detection in cattle. J Parasitol 86:185–188

    Google Scholar 

  • Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425

    CAS  PubMed  Google Scholar 

  • Sparfel JM, Sarfati C, Liguory O, Caroff B, Dumoutier N, Gueglio B, Billaud E, Raffi F, Molina JM, Miegeville M, Derouin F (1997) Detection of microsporidia and identification of Enterocytozoon bieneusi in surface water by filtration followed by specific PCR. J Eukaryot Microbiol 44:78S

    CAS  PubMed  Google Scholar 

  • Sulaiman IM, Fayer R, Lal AA, Trout JM, Schaefer FW, Xiao L (2003) Molecular characterization of microsporidia indicates that wild mammals harbor host-adapted Enterocytozoon spp. as well as human pathogenic Enterocytozoon bieneusi. Appl Environ Microbiol 69:4495–4501

    Article  CAS  PubMed  Google Scholar 

  • Tumwine JK, Kekitiinwa A, Nabukeera N, Akiyoshi DE, Buckholt MA, Tzipori S (2002) Enterozoon bieneusi among children with diarrhea attending Mulago Hospital in Uganda. Am J Trop Med Hyg 67:299–303

    PubMed  Google Scholar 

  • Weber R, Bryan RT, Schwartz DA, Owen RL (1994) Human microsporidial infections. Clin Microbiol Rev 7:426–461

    CAS  PubMed  Google Scholar 

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Acknowledgement

We thank Isabel Fonseca for providing some samples.

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Authors and Affiliations

  1. Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30341, USA

    Irshad M. Sulaiman & Lihua Xiao

  2. Animal and Natural Resources Institute, Agriculture Research Service, Environmental Microbial Safety Laboratory, United States Department of Agriculture, Beltsville, MD 20705, USA

    Ron Fayer

  3. Division of AIDS, STD and TB Laboratory Research, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA

    Chunfu Yang

  4. Unidade de Protozoarios Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Medicas (UPMM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal

    Olga Matos

Authors
  1. Irshad M. Sulaiman
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  2. Ron Fayer
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  3. Chunfu Yang
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  4. Monica Santin
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  5. Olga Matos
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  6. Lihua Xiao
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Corresponding author

Correspondence to Lihua Xiao.

Additional information

An erratum to this article can be found at http://dx.doi.org/10.1007/s00436-004-1100-1

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Sulaiman, I.M., Fayer, R., Yang, C. et al. Molecular characterization of Enterocytozoon bieneusi in cattle indicates that only some isolates have zoonotic potential. Parasitol Res 92, 328–334 (2004). https://doi.org/10.1007/s00436-003-1049-5

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  • DOI: https://doi.org/10.1007/s00436-003-1049-5

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