Abstract
In this work we examined 76 stray dogs from an area of endemic visceral leishmaniosis, in order to determine whether the presence of skin inflammation or a specific inflammatory pattern could be taken as indicative of infection with Leishmania chagasi, and whether the parasite burden in the skin could be associated with the intensity or the nature of the inflammatory process. Inflammatory infiltrates were observed in the skin of 51 out of 55 animals with diagnosis of leishmaniosis, and in 17 out of 21 animals without signs of infection. Amastigotes were identified in the skin of 29 out of the 55 animals with diagnosis of leishmaniosis. Granuloma and a monomorphic macrophage inflammatory infiltrate, and not a mixed focal or mixed diffuse inflammation, were significantly associated with skin parasitism, both in terms of frequency (P=0.015 in the Chi-square test) and intensity (P=0.005 in the Kruskal-Wallis test). A low parasite burden was associated with a multifocal inflammatory pattern.
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Amato VS, de Andrade HF, Duarte MI (2003) Mucosal leishmaniasis: in situ characterization of the host inflammatory response, before and after treatment. Acta Trop 85:39–49
Anstead GM, Chandrasekar B, Zhao W, Yang J, Perez LE, Melby PC (2001) Malnutrition alters the innate immune response and increases early visceralization following Leishmania donovani infection. Infect Immun 69:4709–4718
Ashford DA, Badaró R, Eulálio C, Freire M, Miranda C, Zalis MG, David JR (1993) Studies on the control of visceral leishmaniasis: validation of the Falcon assay screening test–enzyme-linked immunosorbent assay (FAST-ELISA) for field diagnosis of canine visceral leishmaniasis. Am J Trop Med Hyg 48:1–8
Ashford DA, Bozza M, Freire M, Miranda JC, Sherlock I, Eulalio C, Lopes U, Fernandes O, Degrave W, Barker RH Jr, Badaró R, David JR (1995) Comparison of the polymerase chain reaction and serology for the detection of canine visceral leishmaniasis. Am J Trop Med Hyg 53:251–255
Barbosa AA Jr, Andrade ZA, Reed SG (1987) The pathology of experimental visceral leishmaniasis in resistant and susceptible lines of inbred mice. Braz J Med Biol Res 20:63–72
Barral-Netto M, Machado P, Barral A (1995) Human cutaneous leishmaniasis: recent advances in physiopathology and treatment. Eur J Dermatol 5:104–113
Bertho AL, Santiago MA, Coutinho SG (1994) An experimental model of the production of metastases in murine cutaneous leishmaniasis. J Parasitol 80:93–99
Bittencourt AL, Barral-Netto M (1995) Leishmaniasis. In: Doerr W, Seifert G (eds) Tropical pathology (2nd edn). Springer, Berlin Heidelberg New York, pp 597–651
Chensue SW, Warmington KS, Ruth JH, Lincoln P, Kunkel SL (1995) Cytokine function during mycobacterial and schistosomal antigen-induced pulmonary granuloma formation. Local and regional participation of IFN-gamma, IL-10, and TNF. J Immunol 154:5969–5976
Deane LM, Deane MP (1955) Leishmaniose visceral urbana (no cão e no homem) em Sobral, Ceará. Hospital 47:75–87
Dye C (1996) The logic of visceral leishmaniosis control. Am J Trop Med Hyg 55:125–130
Enserink M (2000) Infectious diseases. Has leishmaniasis become endemic in the U.S.? Science 290:1881–1883
Ferrer L, Rabanal R, Fondevila D, Ramos JA, Domingo M (1988) Skin lesions in canine leishmaniasis. J Small Anim Pract 29:381–388
Fondevila D, Vilafranca M, Ferr L (1997) Epidermal immunocompetence in canine leishmaniasis. Vet Immunol Immunopathol 56:319–327
Glantz SA (1997) Primer of biostatistics (4th edn). McGraw-Hill, New York, p 473
Herwaldt BL (1999) Lieshmaniasis. Lancet 354:1191–1199
Lemos de Souza V, Ascencao Souza J, Correia Silva TM, Sampaio Tavares Veras P, Rodrigues de-Freitas LA (2000) Different Leishmania species determine distinct profiles of immune and histopathological responses in CBA mice. Microbes Infect 2:1807–1815
Liarte DB, Mendonca IL, Luz FCO, Abreu EAS, Mello GWS, Farias TJC, Ferreira AFB, Millington MA, Costa CHN (2001) QBC for the diagnosis of human and canine American visceral leishmaniasis: preliminary data. Rev Soc Bras Med Trop 34:577–581
Mariano M (1995) The experimental granuloma. A hypothesis to explain the persistence of the lesion. Rev Inst Med Trop São Paulo 37:161–176
Martínez-Moreno A, Moreno T, Martínez-Moreno FJ, Acosta I, Hernández S (1995) Humoral and cell-mediated immunity in natural and experimental canine leishmaniasis. Vet Immunol Immunopathol 48:209–220
Maurício IL, Stothard JR, Miles MA (2000) The strange case of Leishmania chagasi. Parasitol Today 16:188–189
Mozos E, Pérez J, Day MJ, Lucena R, Ginel PJ (1999) Leishmaniosis and generalized dermodicosis in three dogs: a clinical and immunohistochemical study. J Comp Pathol 120:257–268
Oliveira GG, Santoro F, Sadigursky M (1993) The subclinical form of experimental visceral leishmaniasis in dogs. Mem Inst Oswaldo Cruz 88:243–248
Pontes-de-Carvalho L, Santana CC, Soares MB, Oliveira GG, Cunha-Neto E, Ribeiro-dos-Santos R (2002) Experimental chronic Chagas’ disease myocarditis is an autoimmune disease preventable by induction of immunological tolerance to myocardial antigens. J Autoimmun 18:131–138
Postigo C, Llamas R, Zarco C, Rubio R, Pulido F, Costa JR, Iglesias L (1997) Cutaneous lesions in patients with visceral leishmaniasis and HIV infection. J Infect 35:265–268
Prats N, Ferrer L (1995) A possible mechanism in the pathogenesis of cutaneous lesions in canine leishmaniasis. Vet Rec 134:103–104
Silva ES, Gontijo CM, Pacheco RS, Fiúza VO, Brazil RP (2001) Visceral leishmaniasis in the Metropolitan Region of Belo Horizonte, State of Minas Gerais, Brazil. Mem Inst Oswaldo Cruz 96:285–291
Solano-Gallego L, Morell P, Arboix M, Alberola J, Ferrer L (2001) Prevalence of Leishmania infantum infection in dogs living in an area of canine leishmaniasis endemicity using PCR on several tissues and serology. J Clin Microbiol 39:560–563
Springer TA (1995) Traffic signals on endothelium for lymphocyte recirculation and leukocyte emigration. Annu Rev Physiol 57:827–872
Sulahian A, Garin YJ, Pratlong F, Dedet JP, Derouin F (1997) Experimental pathogenicity of viscerotropic and dermotropic isolates of Leishmania infantum from immunocompromised and immunocompetent patients in a murine model. FEMS Immunol Med Microbiol 17:131–138
Tafuri WL, de Oliveira MR, Melo MN, Tafuri WL (2001) Canine visceral leishmaniosis: a remarkable histopathological picture of one case reported from Brazil. Vet Parasitol 96:203–212
Tarantino C, Rossi G, Kramer LH, Perrucci S, Cringoli G, Macchioni G (2001) Leishmania infantum and Neospora caninum simultaneous skin infection in a young dog in Italy. Vet Parasitol 102:77–83
Travi BL, Tabares CJ, Cadena H, Ferro C, Osorio Y (2001) Canine visceral leishmaniasis in Colombia: relationship between clinical and parasitologic status and infectivity for sand flies. Am J Trop Med Hyg 64:119–124
Wilson ME, Sandor M, Blum AM, Young BM, Metwali A, Elliott D, Lynch RG, Weinstock JV (1996) Local suppression of IFN-gamma in hepatic granulomas correlates with tissue-specific replication of Leishmania chagasi. J Immunol 156:2231–229
Acknowledgements
This work was supported by the Fundacao Oswaldo Cruz (PAPES II, grant number 250.250.320) and the Brazilian National Research Council, (CNPq, grant number 52629/96-5). We are indebted to Dr. Lain Pontes de Carvalho (Oswaldo Cruz Foundation) for his careful revision and suggestions for the text.
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dos-Santos, W.L., David, J., Badaró, R. et al. Association between skin parasitism and a granulomatous inflammatory pattern in canine visceral leishmaniosis. Parasitol Res 92, 89–94 (2004). https://doi.org/10.1007/s00436-003-1016-1
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DOI: https://doi.org/10.1007/s00436-003-1016-1