Abstract
The ATP-binding cassette (ABC) proteins are one of the largest evolutionarily conserved families. They are characterized by the presence of highly conserved nucleotide-binding sites (NBS). In the present study, we identified ABC genes in rodent Plasmodia. We queried the Plasmodium yoelii genome with the ABC signature motif and retrieved 15 contigs. Sequences were classified into seven ABC families by BLAST comparison. Conservation of the five signature ABC motifs in the P. yoelii contigs was examined by multi-alignment of the NBS. Expression of the ABC genes was examined during the blood stages of P. yoelii and P. berghei and the hepatocytic stages of P. yoelii. Our results with RT-PCR on total RNA from blood stages demonstrated the expression of 14 ABC genes in P. yoelii and ten in P. berghei. In P. yoelii hepatocytic stages, the expression of four ABC genes was detected.
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Acknowledgements
Plasmodium falciparum and P. yoelii sequences were provided by the Sanger Centre (UK), the TIGR Genome Project and Stanford Genome Technology Center (USA) and the University of Florida GST and EST Project. We thank Fidel Zavala (New York University) for generously providing the P. yoelii hepatocytic cDNA. We are also grateful to Miryam Garcia for her technical support. This work was supported by NIGMS/MBRS award GM08224 and RCMI award RR-03051 of the NCRR/NIH. Partial support (A.C.S., J.P.R.) was received from the Office of the Associate Deanship of Biomedical Sciences and Graduate Studies of the Medical Sciences Campus. Research fellowships were provided for A.C.S., J.P.R. and J.V. from MBRS/RISE Program GM61838. In addition, J.P.R. was awarded the NIH-NIAID Individual Predoctoral Fellowship (NRSA A1-10285-01A1). Partial funding for this project was also awarded to I.F.R., A.E.S. and C.T.T. by the Biomedical Research Infrastructure Network (BRIN-PR grant P20 RR16470) and the Alliance for Minority Participation programs. All the experiments described comply with the current laws of the country in which the experiments were performed.
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A.C. Szeto and J. Pérez-Rosado contributed equally to this work
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Szeto, A.C., Pérez-Rosado, J., Ferrer-Rodríguez, I. et al. Identification and expression analysis of ABC genes in Plasmodium yoelii and P. berghei . Parasitol Res 92, 1–11 (2004). https://doi.org/10.1007/s00436-003-1001-8
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DOI: https://doi.org/10.1007/s00436-003-1001-8