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In vitro trypanocidal activity of dibutyltin dichloride and its fatty acid derivatives

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Abstract

Searching for new compounds against pathogenic trypanosomes has been substantially accelerated by the development of in vitro screening assays. In an attempt to explore the chemotherapeutic potential of organotin compounds and to broaden the search for newer trypanocides, fatty acid derivatives of dibutyltin dichloride were synthesized and their in vitro trypanocidal profiles studied on Trypanosoma brucei brucei, Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense. A 24-h time course experiment was conducted with various concentrations of the compounds using a 24-well microtiter plate technique. The compounds tested were trypanocidal in a dose-dependent fashion: inhibiting survival and growth, resulting in irreversible morphological deformation and the eventual death of the parasites. The minimum inhibitory concentrations of the tested diorganotins are at low micromolar ranges: from 0.15–0.75 μM for T. b. brucei, T. b. gambiense and T. b. rhodesiense. These observations suggest that organotin has chemotherapeutic potential.

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Acknowledgements

We are grateful to Dr. and Mrs. Hirumi for their valuable technical advice and personal communication. M.N.S. is a recipient of a Japanese Government Scholarship.

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Authors and Affiliations

  1. Protozoology Department, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki-shi 852-8523, Japan

    M. N. Shuaibu, H. Kanbara & T. Yanagi

  2. Biochemistry Department, Ahmadu Bello University Zaria, Nigeria

    M. N. Shuaibu, D. A. Ameh & A. J. Nok

  3. Chemistry Department, Ahmadu Bello University, Zaria, Nigeria

    J. J. Bonire

  4. Electron Microscopy Shop Central Laboratory, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki-shi 852-8523, Japan

    A. Ichinose

Authors
  1. M. N. Shuaibu
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  2. H. Kanbara
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  3. T. Yanagi
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  4. A. Ichinose
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  5. D. A. Ameh
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  6. J. J. Bonire
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  7. A. J. Nok
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Shuaibu, M.N., Kanbara, H., Yanagi, T. et al. In vitro trypanocidal activity of dibutyltin dichloride and its fatty acid derivatives. Parasitol Res 91, 5–11 (2003). https://doi.org/10.1007/s00436-003-0861-2

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  • DOI: https://doi.org/10.1007/s00436-003-0861-2

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