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Structure of the nervous system in the tornaria larva of Balanoglossus proterogonius (Hemichordata: Enteropneusta) and its phylogenetic implications

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Abstract

The tornaria larva of hemichordates occupies a central position in phylogenetic discussions on the relationships between Echinodermata, Hemichordata, and Chordata. Dipleurula-type larvae (tornaria and echinoderm larvae) are considered to be primary in the life cycle and thus provide a model for the ancestral animal common to all three taxa (the theory of W. Garstang). If the similarities between tornaria and the larvae in Echinodermata result from homology, their nervous systems should be basically similar as well. The present study utilizes anti-serotonin and FMRFamide antisera together with laser scanning microscopy, and transmission electron microscopy, to describe in detail the nervous system of the tornaria of Balanoglossus proterogonius. Serotonin immunoreactive neurons were found in the apical and esophageal ganglia, and in the stomach epithelium. FMRFamide immunoreactive neurons, probably sensory in nature, were detected in the apical ganglion and in the equatorial region of the stomach epithelium. At the ultrastructural level, the apical organ consists of a columnar epithelium of monociliated cells and includes a pair of symmetrical eyespots. The apical ganglion is located at its base and has a well-developed neuropil. Different types of neurons are described in the apical organ, esophagus, and stomach. Comparison with larvae in Echinodermata shows several significant differences in the way the larval nervous system is organized. This calls into question the homology between tornariae and echinoderm larvae. The possibility of convergence between the two larval types is discussed.

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Acknowledgements

The authors would like to thank Dr. S. Dautov (Institute of Marine Biology, Vladivostok) for providing us with tornaria larvae, Dr. E. Voronezhskaya (Institute of Developmental Biology, Moscow) for help with immunostaining procedures and fruitful discussions, and M. Claeys (University of Ghent) and D. Fomin (Institute of Marine Biology) for technical assistance with electron microscopy. This study was funded in part by the Support for Science Schools grant N 1219.2003.4 for V.V.Y.

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  1. Leonid P. Nezlin

    Present address: Department of Neurophysiology, University of Göttingen, Humboldtallee 23, 37073, Göttingen, Germany

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  1. Institute of Developmental Biology, Russian Academy of Sciences, 26 Vavilov Street, 117808, Moscow, Russia

    Leonid P. Nezlin

  2. Institute of Marine Biology, Far East Branch of the Russian Academy of Sciences, 690041, Vladivostok, Russia

    Vladimir V. Yushin

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Nezlin, L.P., Yushin, V.V. Structure of the nervous system in the tornaria larva of Balanoglossus proterogonius (Hemichordata: Enteropneusta) and its phylogenetic implications. Zoomorphology 123, 1–13 (2004). https://doi.org/10.1007/s00435-003-0086-z

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