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Impact of ischemia-reperfusion injury on dimensional changes of hepatic microvessels

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Research in Experimental Medicine

Abstract

Dimensional alteration of hepatic microvessels was demonstrated during reperfusion after normothermic hepatic ischemia. Using a specially designed cover glass, it was possible to relocate selected sites of observation and microvessels repeatedly throughout the whole reperfusion time. Twenty minutes of hepatic ischemia resulted in a decrease of sinusoidal diameter (mean±SEM; 10.0±0.3 μm at baseline, 8.2±0.2 μm after ischemia) and diameter of postsinusoidal venules (26.4±1.2 at baseline, 23.0±1.0 after ischemia). In the control group (no ischemia induced) no changes of these parameters were observed. Thus, the reduction of hepatic microvascular cross section was present during the early phase of reperfusion. Hepatic dysfunction was characterized by increased serum activity of liver enzymes and reduction of bile flow in the ischemia-exposed animals. It has been suggested that postischemic dimensional microvascular changes are involved in postischemic liver dysfunction.

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Authors and Affiliations

  1. Institute for Surgical Research, Klinikum Grosshadern, University of Munich, Marchioninistrasse 15, D-81377, Munich, Germany

    T. Kondo & K. Messmer

  2. Department of Surgery, Institute of Clinical Medicine, University of Tsukuba, Tsukuba, Japan

    T. Todoroki

  3. National Institute of Bioscience and Human Technology, Tsukuba, Japan

    T. Hirano

  4. Department of Surgery, Klinikum Grosshadern, University of Munich, Munich, Germany

    F. W. Schildberg

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  1. T. Kondo
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  2. T. Todoroki
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  3. T. Hirano
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  4. F. W. Schildberg
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  5. K. Messmer
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Correspondence to T. Kondo.

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Kondo, T., Todoroki, T., Hirano, T. et al. Impact of ischemia-reperfusion injury on dimensional changes of hepatic microvessels. Res. Exp. Med. 198, 63–72 (1998). https://doi.org/10.1007/s004330050090

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  • DOI: https://doi.org/10.1007/s004330050090

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