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Clinical value and influencing factors of establishing stomach cancer organoids by endoscopic biopsy

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Abstract

Objective

To establish an in vitro study model of gastric cancer, gastric cancer organoid culture system, by biopsy under gastroscope, and to explore and analyze the related factors affecting the success rate of culture, to provide a better in vitro model for the study of gastric cancer.

Methods

Twenty-six patients with advanced gastric cancer were collected. Organoids were cultured by biopsy under gastroscope. Paraffin sections were made and HE staining was used to compare the consistency of gastroscopic pathological morphology and organoids. To explore the influencing factors of cultivating gastric cancer organoids in combination with clinical data.

Results

A total of 26 cases were collected by gastroscopy, and 12 cases of gastric cancer organoids were successfully cultured after identification, with a success rate of about 48%. Its histopathological morphology was highly consistent with that of gastric cancer. According to the pathological type, 21 cases were poorly differentiated adenocarcinoma and 12 cases were successful. Four cases of signet ring cell carcinoma failed. According to the location of the lesion, the success rate of sampling and culture of gastric antrum was significantly lower, which may be related to antral edema and anatomical characteristics of gastric antrum. Some of the failed cases are related to the quality of sampling, technique and contamination of tissue cells.

Conclusions

We have successfully established gastric cancer organoids through endoscopic biopsy, and analyzed the factors affecting the success rate of culture from various angles, to improve and enhance the organoid culture technology and provide a better platform for tumor research.

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Availability of data and materials

The data and materials supporting the conclusions of the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We thank Wanying Cheng and Ke Yang for providing assistance in immunohistochemistry staining.

Funding

This work was supported by grants from Shanghai science committee (19411970700), Changhai Hospital Affiliated to Naval Medical University (2019YXK019) and Youth Foundation of Naval Medical University (2021QN26).

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Author notes

  1. Jie Li, Yan Chen, and Yingyi Zhang contributed equally to this work.

Authors and Affiliations

  1. Department of Oncology, Changhai Hospital, Naval Medical University, 168 Changhai Road, Shanghai, 200433, People’s Republic of China

    Jie Li, Yingyi Zhang, Xiaobo Peng, Meihong Wu, Ling Chen & Xianbao Zhan

  2. Department of Gastroenterology, Changhai Hospital, Naval Medical University, 168 Changhai Road, Shanghai, 200433, People’s Republic of China

    Yan Chen

Authors
  1. Jie Li
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  2. Yan Chen
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  5. Meihong Wu
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  6. Ling Chen
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Contributions

Conception and design: XZ. Collection of samples and data: JL and YC. Data collection and arrangement: JL and MW. Data analysis and interpretation: YZ and XP. Manuscript writing: JL and LC. Study supervision and revision of the manuscript: XZ. Final approval of manuscript: All authors. Accountable for all aspects of the work: All authors.

Corresponding author

Correspondence to Xianbao Zhan.

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Li, J., Chen, Y., Zhang, Y. et al. Clinical value and influencing factors of establishing stomach cancer organoids by endoscopic biopsy. J Cancer Res Clin Oncol 149, 3803–3810 (2023). https://doi.org/10.1007/s00432-022-04296-4

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