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Condensed chromatin staining of CKAP2 as surrogate marker for mitotic figures

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Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

Purpose

Proliferation activity has long been known to be one of the strongest prognostic factors in many different cancers. Nevertheless, microscopic evaluation of mitotic figures remains time-consuming and, furthermore, is relatively subjective. As the expression of cytoskeleton-associated protein 2 (CKAP2) is closely related to the mitotic phase, CKAP2 was evaluated as a surrogate mitotic figure (MF) marker.

Methods

A monoclonal antibody specific to human CKAP2 was produced, and immunohistochemistry was performed on normal tissue array sections and 30 breast cancer tissues.

Results

The expression of CKAP2 in the normal human tissues was limited to well-known cell proliferation zones. Strong, readily visible, condensed chromatin staining of CKAP2 was observed specifically in mitotic cells, and the number of these cells was tightly correlated with the MF count in breast cancer tissues (P < 0.001, ρ = 0.743), suggesting its usefulness as a surrogate marker for MF counting.

Conclusion

Immunohistochemical staining with CKAP2 monoclonal antibody can be considered to be a new, effective approach to the assessment of proliferation activity in cancer tissues.

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Abbreviations

CKAP2:

Cytoskeleton-associated protein 2

MF:

Mitotic figure

FFPE:

Formalin-fixed paraffin-embedded

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Acknowledgments

This work was supported by research grants (nos. 0810240 and 1110130) from the National Cancer Center, Korea, to K.M.H., by grants from the National R&D Program for Cancer Control, Ministry of Health & Welfare, Korea, (no. 0720370) and from Samsung Biomedical Research Institute, Korea (no. BB01022), to C.D.B., and by a grant (2011) from Inje University, Korea, to H.S.K.

Conflict of interest

We declare that we have no conflict of interest.

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Authors and Affiliations

  1. Department of Pathology, Ilsan Paik Hospital, Inje University, Ilsanseo-gu, Goyang, 411-702, Korea

    Han-Seong Kim

  2. Research Institute, National Cancer Center, 111 Jungbalsan-ro, 323 Ilsan-ro, Ilsandong-gu, Goyang, 410-769, Korea

    Yong-Bock Choi, Jung-Hwa Lee, Seong-Yeol Park, Hyun-Kyoung Kim, Kyung-Tae Kim & Kyeong-Man Hong

  3. Department of Pathology, Korea Cancer Center Hospital, 75 Nowon-gil, Nowon-gu, Seoul, 139-706, Korea

    Jae-Soo Koh

  4. Department of Pathology, Kwandong University College of Medicine, Myongji Hospital, Deokyang-gu, Goyang, 697-24, Korea

    Sang-Yeop Yi

  5. Department of Molecular Cell Biology and Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, 300 Chunchundong, Jangangu, Suwon, 440-746, Korea

    Kyung-Uk Hong, Joobae Park & Chang-Dae Bae

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  1. Han-Seong Kim
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Correspondence to Chang-Dae Bae or Kyeong-Man Hong.

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Kim, HS., Choi, YB., Lee, JH. et al. Condensed chromatin staining of CKAP2 as surrogate marker for mitotic figures. J Cancer Res Clin Oncol 138, 95–102 (2012). https://doi.org/10.1007/s00432-011-1053-6

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  • DOI: https://doi.org/10.1007/s00432-011-1053-6

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