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Overexpression of Galectin-1 is negatively correlated with pathologic differentiation grade in oral squamous cell carcinoma

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Abstract

Purpose

To determine the Galectin-1 protein expression in oral squamous cell carcinoma (OSCC).

Methods

Comparative proteomic analysis of an in vitro cellular carcinogenesis model of OSCC we previously established was performed to identify differentially expressed proteins. Galectin-1 was further validated in vitro (human immortalized oral epithelia cell line and OSCC lines) and in vivo (tissue samples from OSCC patients) by Western blotting and immunohistochemistry, respectively.

Results

Increased Galectin-1 protein expression was identified in the cancerous cell line compared with the immortalized oral epithelial cell line in the in vitro cellular carcinogenesis model, and then validated in the OSCC lines and cancerous tissues. Galectin-1 protein expression was negatively correlated with the tumor pathologic differentiation grades, a higher Galectin-1 protein expression indicating a poorer pathologic differentiation grade.

Conclusions

Galectin-1 protein expression level increases in OSCC, it may serve as a candidate marker for pathologic differentiation grade of OSCC.

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Acknowledgments

These works were supported by research grants 30973344 and 30700953 from National Natural Science Foundation of China; by research grant 2007CG22 from Shanghai Educational Development Foundation; by research grant (08QA14056) from Shanghai Rising-Star Program; by research grant 2007BAI18B03 from National Key Technology R&D Program of China; and by research grant 20060400168 from China Postdoctoral Science Foundation.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, Ninth People’s Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Stomatology, Shanghai, People’s Republic of China

    Lai-ping Zhong, Kui-jie Wei, Xiao Yang, Hong-ya Pan, Dong-xia Ye, Li-zhen Wang & Zhi-yuan Zhang

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  1. Lai-ping Zhong
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  2. Kui-jie Wei
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  3. Xiao Yang
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  4. Hong-ya Pan
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  5. Dong-xia Ye
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  6. Li-zhen Wang
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  7. Zhi-yuan Zhang
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Correspondence to Zhi-yuan Zhang.

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Zhong, Lp., Wei, Kj., Yang, X. et al. Overexpression of Galectin-1 is negatively correlated with pathologic differentiation grade in oral squamous cell carcinoma. J Cancer Res Clin Oncol 136, 1527–1535 (2010). https://doi.org/10.1007/s00432-010-0810-2

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  • DOI: https://doi.org/10.1007/s00432-010-0810-2

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