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Semi-quantitative detection of GADD45-gamma methylation levels in gastric, colorectal and pancreatic cancers using methylation-sensitive high-resolution melting analysis

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Abstract

Purpose

GADD45 is a family of proteins involved in DNA damage response and cell growth arrest. GADD45G was identified as an interleukin-2-induced immediate-early gene, and methylation of GADD45G was studied in various tumor cell lines and a few primary tumor samples. High-resolution melting (HRM) analysis has been used as a novel tool for analysis of promoter methylation.

Methods

In our study, we used HRM analysis to detect the methylation levels of GADD45G gene in 100 gastric cancers, 100 colorectal cancers, 70 pancreatic cancers and equal number of adjacent normal tissues.

Results

The frequency of GADD45G methylation in all three types of cancers was significantly higher than that in normal tissues. Consistent with previous reports, expression levels of GADD45G were inversely correlated with methylation levels. But we did not find significant association between GADD45G methylation status and TNM staging in all three types of cancers.

Conclusions

In summary, application of HRM analysis to large amount of clinical samples proves to be a fast and high-throughput way to investigate the epigenetic status of GADD45G. And this is the first study to evaluate the prevalence of GADD45G methylation based on large amount of tumor samples, showing that epigenetic regulation of GADD45G was associated with carcinogenesis.

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Conflict of interest statement

We declare that we have no conflict of interest.

Author information

Authors and Affiliations

  1. JNU-HKUST Joint Lab, Jinan University, Guangdong, China

    Wei Zhang

  2. Department of Intervention and Vascular Surgery, Peking University Third Hospital, Beijing, China

    Tianrun Li

  3. Department of Dermatology, Shenzhen Hospital Peking University, No. 1120, Lian-Hua Road, Fu-Tian District, Shenzhen, Guangdong, China

    Yong Shao, Jie Zhang & Bo Yu

  4. Biomedical Research Institute, Shenzhen-PKU-HKUST Medical Center, Shenzhen, Guangdong, China

    Yong Shao, Chao Zhang, Qi Wu, Bo Yu & Jun Wan

  5. Department of Clinical Laboratory, Shenzhen Hospital Peking University, Shenzhen, Guangdong, China

    Hong Yang

  6. Central Laboratory, Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, China

    Ming Guan

  7. Department of Biochemistry, The Hong Kong University of Science and Technology, Hong Kong, China

    Jun Wan

Authors
  1. Wei Zhang
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  2. Tianrun Li
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  3. Yong Shao
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  4. Chao Zhang
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  5. Qi Wu
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  6. Hong Yang
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  7. Jie Zhang
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  8. Ming Guan
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  9. Bo Yu
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  10. Jun Wan
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Corresponding authors

Correspondence to Bo Yu or Jun Wan.

Additional information

W. Zhang, T. Li, B. Yu, J. Wan contributed equally to this work.

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Zhang, W., Li, T., Shao, Y. et al. Semi-quantitative detection of GADD45-gamma methylation levels in gastric, colorectal and pancreatic cancers using methylation-sensitive high-resolution melting analysis. J Cancer Res Clin Oncol 136, 1267–1273 (2010). https://doi.org/10.1007/s00432-010-0777-z

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  • DOI: https://doi.org/10.1007/s00432-010-0777-z

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