Abstract
Purpose
The polymorphisms in microRNA (miRNA) machinery genes and miRNA-containing genomic regions may play an important role in cancer development and prognosis. Accordingly, the present study analyzed the single nucleotide polymorphisms (SNPs) of miRNA-related genes and their impact on the prognosis for patients with colorectal cancer.
Methods
Four hundred and twenty-six consecutive patients with surgically treated colorectal adenocarcinoma were enrolled. The genomic DNA was extracted from fresh colorectal tissue and 40 polymorphisms of miRNA-related genes determined using a real-time PCR genotyping assay.
Results
In a univariate analysis, the progression-free survival of the patients with the combined mir492 C/G and G/G genotype was significantly worse than that of the patients with the mir492 C/C genotype (rs2289030) (P value = 0.0426), although there was no difference in the overall survival. However, no association was noted between the SNPs of the miRNA-related genes evaluated and survival in a multivariate analysis.
Conclusions
None of the 40 miRNA-related gene polymorphisms investigated in this study was found to be an independent prognostic marker for Korean patients with surgically resected colorectal cancer. However, further studies are warranted to clarify the role of miRNA-related gene polymorphisms as a prognostic biomarker for colorectal cancer patients.
Similar content being viewed by others
References
Bartel DP (2004) MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 116:281–297
Brendle A, Lei H, Brandt A, Johansson R, Enquist K, Henriksson R, Hemminki K, Lenner P, Forsti A (2008) Polymorphisms in predicted microRNA-binding sites in integrin genes and breast cancer: ITGB4 as prognostic marker. Carcinogenesis 29:1394–1399
Calin CA, Croce CM (2006) MicroRNA signatures in human cancers. Nat Rev Cancer 6:857–866
Calin GA, Ferracin M, Cimmino A, Di Leva G, Shimizu M, Wojcik SE, Iorio MV, Visone R, Sever NI, Fabbri M, Iuliano R, Palumbo T, Pichiorri F, Roldo C, Garzon R, Sevignani C, Rassenti L, Alder H, Volinia S, Liu CG, Kipps TJ, Negrini M, Croce CM (2005) A micro-RNA signature associated with prognosis and progression in chronic lymphocytic leukemia. N Engl J Med 353:1793–1801
Diederichs S, Haber DA (2006) Sequence variations of microRNAs in human cancer: alterations in predicted secondary structure do not affect processing. Cancer Res 66:6097–9104
Duan R, Pak C, Jin P (2007) Single nucleotide polymorphism associated with mature miR-125a alters the processing of pri-miRNA. Hum Mol Genet 16:1124–1131
Esquela-Kerscher A, Slack FJ (2006) Oncomirs-microRNAs with a role in cancer. Nat Rev Cancer 6:259–269
Greene FL, Page DL, Fleming ID (2002) The AJCC cancer staging manual, 6th edn edn. Springer, New York
Hamilton SR, Aaltonen LA (2000) WHO classification. Tumours of the digestive system: pathology and genetics. IARC Press, Lyon
Hirokawa Y, Wood CG, Yang H, Zhao H, Ye Y, Gu J, Lin J, Habuchi T, Wu X (2008) Single nucleotide polymorphisms of microRNA machinery genes modify the risk of renal cell carcinoma. Clin Cancer Res 14:7956–7962
Iwai N, Naraba H (2005) Polymorphisms in human pre-miRNAs. Biochem Biophysics Res Commun 331:1439–1444
Landi D, Gemignani F, Naccarati A, Pardini B, Vodicka P, Vodickova L, Novotny J, Forsti A, Hemminki K, Federico C, Landi S (2008) Polymorphisms within micro-RNA-binding sites and risk of sporadic colorectal cancer. Carcinogenesis 29:579–584
Passetti F, Ferreira CG, Costa FF (2009) The impact of microRNAs and alternative splicing in pharmacogenomics. Pharmacogenomics J 9:1–13
Popat S, Hubner R, Houlston RS (2005) Systematic review of microsatellite instability and colorectal cancer prognosis. J Clin Oncol 23:609–618
Saunders MA, Liang H, Li WH (2007) Human polymorphism at microRNAs and microRNA target sites. Proc Natl Acad Sci USA 104:3300–3305
Conflict of interest statement
All the authors declare there were no any financial and personal relationships with other people or organizations that could inappropriately influence (bias) this investigational work.
Author information
Authors and Affiliations
Corresponding authors
Additional information
J. G. Kim and G. S. Choi contributed equally to this work.
Rights and permissions
About this article
Cite this article
Lee, HC., Kim, J.G., Chae, Y.S. et al. Prognostic impact of microRNA-related gene polymorphisms on survival of patients with colorectal cancer. J Cancer Res Clin Oncol 136, 1073–1078 (2010). https://doi.org/10.1007/s00432-009-0754-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00432-009-0754-6