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Increased H2O2 level in exhaled breath condensate in primary breast cancer patients

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Abstract

Purpose

This study was designed to assess exhaled hydrogen peroxide (H2O2), blood serum antioxidant capacity, and tumor necrosis factor-α (TNFα) in primary breast cancer (PBC).

Methods

The study included 34 consecutive, non-smoking PBC patients (aged 62.5 ± 13.5 at surgery) prior to the treatments, qualified for modified radical mastectomy and not undergoing any adjuvant systemic therapy, and 33 healthy controls. The post surgery pathological assessment included tissue expression of estrogen (ER) and progesterone (PR) receptors, and epidermal growth factor receptor type 2 (HER-2/neu). Exhaled H2O2 was determined fluorometrically in the exhaled breath condensate (EBC). Blood serum antioxidant capacity and TNFα levels were assessed with ferric reducing ability of plasma (FRAP) and ELISA immunoassay, respectively.

Results

In PBC patients, 10 ER, 11 PR, and 9 HER-2/neu positive tumors were identified and HER-2/neu score was 2+ in 20% of all tumors. Median (Me) H2O2 was increased up to 0.44 μM (interquartile range IR: 0.20–1.25 μM) compared with healthy control of 0.36 μM (IR: 0.12–0.48 μM; p < 0.05). The H2O2 concentration in EBC was significantly correlated (τ = 0.27; p = 0.03) and increased in cases with nodal metastases (n = 12; p = 0.04). Serum TNFα was increased up to 51.7 ± 21.0 pg/ml compared with controls 17.2 ± 3.65 pg/ml (p < 0.05). FRAP was increased to 1.41 ± 0.37 mM Fe2+ compared with control 1.19 ± 0.17 mM Fe2+; (p = 0.006).

Conclusions

This is the first study to demonstrate increased H2O2 in exhaled breath condensate in patients with localized breast malignancy and its relation with clinical severity.

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Abbreviations

EBC:

Exhaled breath condensate

FRAP:

Ferric reducing ability of plasma

HER:

-2/neu human epidermal growth factor cell surface receptor-2

IHC:

Immunohistochemistry

PBC:

Primary breast cancer

ROS:

Reactive oxygen species

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Authors and Affiliations

  1. Department of Clinical Physiology, Medical University of Lodz, Mazowiecka St. 6/8, 92-215, Lodz, Poland

    Dariusz Nowak

  2. Department of Cardiovascular Physiology, Medical University of Lodz, Mazowiecka St. 6/8, 92-215, Lodz, Poland

    Robert A. Stolarek

  3. Department of Oncological Surgery, Medical University of Lodz, Paderewskiego St. 4, 93-509, Lodz, Poland

    Jarosław Jakubik & Arkadiusz Jeziorski

  4. Department of Cellular Interactions, Medical University of Lodz, Mazowiecka St. 6/8, 92-215, Lodz, Poland

    Elżbieta Potargowicz & Ewa Sęklewska

  5. Center for High School Education, Traugutta St. 1, 95-035, Ozorkow, Poland

    Marek Lewandowski

Authors
  1. Robert A. Stolarek
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  2. Elżbieta Potargowicz
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  3. Ewa Sęklewska
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  4. Jarosław Jakubik
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Correspondence to Dariusz Nowak.

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Stolarek, R.A., Potargowicz, E., Sęklewska, E. et al. Increased H2O2 level in exhaled breath condensate in primary breast cancer patients. J Cancer Res Clin Oncol 136, 923–930 (2010). https://doi.org/10.1007/s00432-009-0734-x

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  • DOI: https://doi.org/10.1007/s00432-009-0734-x

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