Abstract
Introduction
MiRNAs are short single stranded RNAs that are associated with gene regulation at the transcriptional and translational level. Changes in their expression were found in a variety of human cancers. In the present study, we aimed to investigate expression profiles of miRNA (miRNA) in the clear cell subtype of kidney cancer and to develop further understanding of the molecular mechanisms involved in the pathogenesis of renal cell carcinoma.
Materials and methods
We analyzed the miRNA expression profiles in 30 pairs of renal cell carcinoma and adjacent nontumorous tissue (NT), using a mammalian miRNA microarray containing whole human mature and precursor miRNA sequences. Real-time quantitative PCR was performed to confirm the array results.
Results
The miRNA microarray chip analysis identified 86 miRNAs differentially expressed in renal cell carcinoma tissues and a total of 38 miRNAs exhibited higher expression in the renal cell carcinoma samples than that in the NT samples, while 48 miRNA demonstrated lower expression in the renal cell carcinoma samples than that in the NT samples.
Conclusion
Quantitative real-time PCR analysis confirmed microarray data. The report supports that many miRNA expressions were altered in renal carcinoma, whose expression profiling may provide a useful clue for the pathophysiology research. However, further longer-term researches are required to investigate the relationship between miRNA and renal carcinoma as well as their role in carcinogenesis.
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Acknowledgments
This work was supported by the grant from Shandong Provincial Education Department Foundation of China (No. J07WZ29) and Dr. initiation fund from Weifang Medical University. The authors thank the patients who participated in this study. The microarray experiments were performed by KangChen Bio-tech, Shanghai, China.
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The authors declare that they have no conflict of interest.
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Z. Yi and Y. Fu contributed equally to this work.
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Yi, Z., Fu, Y., Zhao, S. et al. Differential expression of miRNA patterns in renal cell carcinoma and nontumorous tissues. J Cancer Res Clin Oncol 136, 855–862 (2010). https://doi.org/10.1007/s00432-009-0726-x
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DOI: https://doi.org/10.1007/s00432-009-0726-x