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The importance of −460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer

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Abstract

Purpose

The present study investigated the functional influence of the single nucleotide polymorphisms (SNPs) −460 C/T and +405 G/C at vascular endothelial growth factor A (VEGF-A), mRNA and protein levels in colorectal cancer (CRC) and normal colorectal tissue.

Methods

Blood and tissue were collected from 113 patients surgically resected for colorectal cancer. SNPs were analysed from genomic DNA by PCR, the VEGF-A gene expression analysis was performed by RT–PCR and protein analysis by ELISA.

Results

The T-allele in the −460 C/T SNP and the C-allele in the +405 G/C SNP were associated with significantly lower VEGF-A protein levels in normal colorectal tissue. There were no differences in protein levels in the malignant tissue according to genotypes. No differences were observed at the gene expression levels either.

Conclusion

The results indicate that the two SNPs have a functional influence on the VEGF-A protein levels in normal colorectal tissue. The possible clinical implications of the findings need further investigation.

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Acknowledgements

We are very thankful for the technical assistance provided by Lone Frischknecht, Lone Hartmann Hansen, Sara Egsgaard and Birgit Roed Sørensen and for the proofreading by Karin Larsen.

Conflict of interest statement

None of the authors had any conflicts of interest.

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Authors and Affiliations

  1. Vejle Hospital, Vejle, Denmark

    Torben F. Hansen, Karen-Lise G. Spindler, Karen A. Lorentzen, Dorte A. Olsen, Rikke F. Andersen, Jan Lindebjerg, Ivan Brandslund & Anders Jakobsen

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  1. Torben F. Hansen
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  2. Karen-Lise G. Spindler
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  3. Karen A. Lorentzen
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  4. Dorte A. Olsen
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  5. Rikke F. Andersen
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  6. Jan Lindebjerg
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  7. Ivan Brandslund
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  8. Anders Jakobsen
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Correspondence to Torben F. Hansen.

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Hansen, T.F., Spindler, KL.G., Lorentzen, K.A. et al. The importance of −460 C/T and +405 G/C single nucleotide polymorphisms to the function of vascular endothelial growth factor A in colorectal cancer. J Cancer Res Clin Oncol 136, 751–758 (2010). https://doi.org/10.1007/s00432-009-0714-1

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  • DOI: https://doi.org/10.1007/s00432-009-0714-1

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