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Characterization of intracellular superoxide dismutase alterations in premalignant and malignant lesions of the oral cavity: correlation with lymph node metastasis

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Abstract

Purpose

The purpose of this study was to characterize changes in the expression of copper–zinc superoxide dismutase (Cu/Zn-SOD) and manganese SOD (Mn-SOD) in oral squamous-cell carcinoma (OSCC).

Methods

Real-time quantitative reverse transcriptase-polymerase chain reaction analysis of Cu/Zn-SOD and Mn-SOD mRNA expression was carried out in 50 pairs of OSCC tissue specimens and corresponding normal tissues. Mn-SOD protein expression was evaluated further in 65 OSCC tissue samples and 33 oral premalignant lesions (OPLs) using immunohistochemistry.

Results

Significant (P < 0.001) upregulation of Mn-SOD mRNA expression was observed in OSCC tissues compared with the normal tissue counterparts, whereas no significant difference was detected in Cu/Zn-SOD expression. Significant increases in Mn-SOD protein expression were seen in both OPLs (P < 0.001) and OSCC tissue (P < 0.001) together with a high incidence of lymph node metastasis (P = 0.04).

Conclusions

Our findings suggested that Mn-SOD overexpression is a frequent and early event during oral carcinogenesis and could contribute to aggressive OSCC.

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Acknowledgments

We thank Lynda C. Charters for editing this manuscript. This study was partly supported by a Grant-in-Aid Scientific Research (No. 20791492) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and Global COE Program (Global Center for Education and Research in Immune System Regulation and Treatment), MEXT, Japan.

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Authors and Affiliations

  1. Department of Clinical Molecular Biology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Hitomi Nomura, Yukio Yamano, Kazuaki Fushimi, Katsunori Ogawara, Masashi Shiiba, Katsuhiro Uzawa & Hideki Tanzawa

  2. Department of Respirology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Yuichi Takiguchi

  3. Division of Dentistry and Oral-Maxillofacial Surgery, Chiba University Hospital, 1-8-1 Inohana, Chuo-ku, Chiba, 260-8670, Japan

    Hidetaka Yokoe, Yosuke Sakamoto, Hiroki Bukawa, Katsuhiro Uzawa & Hideki Tanzawa

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  1. Hidetaka Yokoe
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  2. Hitomi Nomura
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  3. Yukio Yamano
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Correspondence to Hideki Tanzawa.

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H. Yokoe, H. Nomura contributed equally to this work.

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Yokoe, H., Nomura, H., Yamano, Y. et al. Characterization of intracellular superoxide dismutase alterations in premalignant and malignant lesions of the oral cavity: correlation with lymph node metastasis. J Cancer Res Clin Oncol 135, 1625–1633 (2009). https://doi.org/10.1007/s00432-009-0610-8

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  • DOI: https://doi.org/10.1007/s00432-009-0610-8

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